Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
31
0
1

Year Published

2005
2005
2016
2016

Publication Types

Select...
8

Relationship

6
2

Authors

Journals

citations
Cited by 91 publications
(32 citation statements)
references
References 23 publications
0
31
0
1
Order By: Relevance
“…The brain was removed on the cold, the hippocampus was isolated, placed into liquid nitrogen, and stored at -66 o C. Total RNA was isolated and qualitative reverse transcription PCT were carried out as described previously [10]. The level of 5-HT 1A receptor mRNA expression in the hippocampus was presented as the number of copies per 100 copies of a house keeping gene (glyceraldehyde-3-phosphate dehydrogenase), which served as the internal standard for reverse transcription.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The brain was removed on the cold, the hippocampus was isolated, placed into liquid nitrogen, and stored at -66 o C. Total RNA was isolated and qualitative reverse transcription PCT were carried out as described previously [10]. The level of 5-HT 1A receptor mRNA expression in the hippocampus was presented as the number of copies per 100 copies of a house keeping gene (glyceraldehyde-3-phosphate dehydrogenase), which served as the internal standard for reverse transcription.…”
Section: Methodsmentioning
confidence: 99%
“…The level of 5-HT 1A receptor mRNA expression in the hippocampus was presented as the number of copies per 100 copies of a house keeping gene (glyceraldehyde-3-phosphate dehydrogenase), which served as the internal standard for reverse transcription. C57BL/6 mouse DNA served as the external reference for evaluating the number of mRNA copies of the appropriate genes per 1 µl cDNA [10].…”
Section: Methodsmentioning
confidence: 99%
“…One microgram of total RNA was taken for cDNA synthesis with a random hexanucleotide mixture [25]. The concentration of genomic DNA in the cDNA samples did not exceed 30 copies/l.…”
Section: Rt-pcrmentioning
confidence: 99%
“…Reagent controls were carried out under the same conditions but without the template. Gene expression was evaluated as the number of cDNA copies with respect to 100 copies of rPol II cDNA [25][26][27]. Melting curve analysis was performed at the end of each run for each primer pair, allowing us to control amplification specificity.…”
Section: Rt-pcrmentioning
confidence: 99%
“…Listed below are the most commonly applied external standards: (1) artificially cloned fragment of the target gene DNA, containing annealing sites for the primers used for evaluation of the target gene expression (Lole and Arankalle, 2006); (2) genomic DNA (Cuscó et al, 2002; (Zhao et al, 2006). Genomic DNA is the most universal since it represents all the genes of an organism (Cuscó et al, 2002;Kulikov et al, 2005). Furthermore, calibration curves that are obtained using genomic DNA are far more stable than those obtained using amplicons, which allows comparing results of different experiments.…”
Section: Introductionmentioning
confidence: 99%