Quantitative proteomics reveals key pathways in the symbiotic interface and the likely extracellular property of soybean symbiosome

Rapid Changes to Endomembrane System of Infected Root Nodule Cells to Adapt to Unusual Lifestyle

“…It is quite plausible that the symbiosome also can be defined as "the extracellular space". The recent data reported by Luo et al [53] provided some support for this speculation. According to the data obtained via label-free quantitative proteomic technology for the soybean symbiosome membrane (SM), peribacteroid space (PBS), and root microsomal fraction (RMF), material exchange and signal communication indicate the likely extracellular nature of the symbiosome [53].…”

“…The recent data reported by Luo et al [53] provided some support for this speculation. According to the data obtained via label-free quantitative proteomic technology for the soybean symbiosome membrane (SM), peribacteroid space (PBS), and root microsomal fraction (RMF), material exchange and signal communication indicate the likely extracellular nature of the symbiosome [53]. Several coatomer proteins of the COPI complex were detected in the SM proteome; the COPI and COPII complexes are known to be involved in retrograde and anterograde trafficking between the ER and Golgi apparatus [53].…”

“…The host cell membrane transporters that have been identified on the symbiosome membrane include the iron transporter [44,45], the iron-activated citrate transporter [46], the molybdenum transporter [47], Zinc-Iron Permease6 [48], the SST1 sulfate transporter [49], copper transporter1 [50], peptide transporter Soybean Yellow Stripe-like 7 [51], and several putative candidates for malate transporters [52]. The recently presented work by Luo et al [53] described a quantitative proteomics analysis of soybean root nodule symbiosome membranes and provided a framework of putative research in transport toward the symbiosome membrane and the regulatory mechanisms of this transport.…”

“…According to the data obtained via label-free quantitative proteomic technology for the soybean symbiosome membrane (SM), peribacteroid space (PBS), and root microsomal fraction (RMF), material exchange and signal communication indicate the likely extracellular nature of the symbiosome [53]. Several coatomer proteins of the COPI complex were detected in the SM proteome; the COPI and COPII complexes are known to be involved in retrograde and anterograde trafficking between the ER and Golgi apparatus [53]. Secretory proteins released into the ER lumen can be retained in this compartment, move along the secretory pathway, be transported to vacuoles in plants (bypassing the Golgi apparatus), or be excreted to the external (extracellular) space [58].…”

Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule symbiosis establishment

“…Subcluster 12-0 is shared by nodules at two different developmental stages but the small subcluster 12-1 is almost exclusively occupied by the 12-dpi immature nodule (Fig.2f). The expression levels of genes encoding symbiosome membrane protein 12 were much higher in cluster 12-0 than in subcluster 12-1, while there was almost no difference between subcluster 12-1 and UCs (Fig.2g), indicating a more active movement of solutes between symbionts in subcluster 12-0 and subcluster 12-1 is a transitional cell type of ICs during nodule development. We checked subcluster 12-1 specific gene and found nearly 12% of the genes (6/50) are included in known SNF genes collected by Roy et al 1 .…”