Quantitative phosphoproteomic analysis reveals γ‐bisabolene inducing p53‐mediated apoptosis of human oral squamous cell carcinoma via HDAC2 inhibition and ERK1/2 activation

Abstract

Microbiology 3320 Absolute quantification of dengue virus serotype 4 chimera vaccine candidate in Vero cell culture by targeted mass spectrometry

Quantitative phosphoproteome analysis of cisplatin‐induced apoptosis in Jurkat T cells

“…Comparing these proteins with our dataset, 253 common proteins were found, but only 34 have been found to be upregulated due to cisplatin‐induced apoptosis, seven downregulated, and 212 unregulated. On‐plate phosphopeptide enrichment using TiO 2 ‐polydimethylsiloxane coated plates was applied to analyze the phosphoproteome profile of human oral squamous cell carcinoma cells by γ‐bisabolene‐induced apoptosis and revealed 358 quantifiable phosphopeptides . Of the 58 proteins reported to be upregulated with fold change more than two, 43 proteins were identified in the present study with 42 peptides covering the same phosphorylated peptide sequences in both studies.…”

“…A few quantitative phosphoproteomics studies of apoptotic cells have been performed . In these studies, different cell lines (bronchial epithelial, ovarian cancer and oral cancer) were studied using different apoptosis inducers (cadmium chloride, gossypol, γ‐bisabolene, and staurosporine) and proteomics approaches (TiO 2 ‐phosphopeptide enrichment/LC‐MS/label‐free quantification, protein array, and PROTOMAP).…”

Antiviral Activity of a Novel Compound CW-33 against Japanese Encephalitis Virus through Inhibiting Intracellular Calcium Overload

“…JEV-infected cells treated with or without CW-33 for 24 h were lysed in RIPA (radioimmunoprecipitation assay) lysis buffer; lysates were dissolved in solutions of 18.5 μL of acrylamide (40%, 29:1), 2.5 μL of 10% ammonium persulfate, and 1 μL of TEMED (tetramethylethylenediamine). Lysate proteins in the acrylamide gel were digested with trypsin; peptides were derived from gel via sequential extraction, and then applied for purification of phosphopeptides and nanoLC-MS/MS analysis, as described above in our prior report [ 43 ]. Phosphopeptides were enriched using the TiO 2 –polydimethylsiloxane (PDMS) coated plate, resuspended in 0.1% FA solution, and then identified with a nanoflow UPLC system (UltiMate 300 RSLCnano system, Dionex, Amsterdam, The Netherlands) coupled with a captive spray ion source and a Q-TOF mass spectrometer (maXis impact, Bruker, Fremont, CA, USA).…”

β‐Bisabolene, a Sesquiterpene from the Essential Oil Extract of Opoponax (Commiphora guidottii), Exhibits Cytotoxicity in Breast Cancer Cell Lines

“…A recent report describing the pro‐apoptotic effects of a closely related isomer, γ‐bisabolene, in oral squamous cell carcinoma cells suggests that bisabolene isomers may share certain chemical features that contribute to their anti‐tumour functions (Jou et al, ). Accordingly, the selective induction of apoptosis in cancer cells that we observed were also demonstrated by γ‐bisabolene against oral squamous carcinoma cells but not normal oral fibroblasts.…”

“…Accordingly, the selective induction of apoptosis in cancer cells that we observed were also demonstrated by γ‐bisabolene against oral squamous carcinoma cells but not normal oral fibroblasts. Mechanistically, γ‐bisabolene was shown to activate p53‐mediated apoptosis through protein phosphatase‐1/histone deacetylase 2 (HDAC2) and extracellular signal‐regulated kinase signalling (Jou et al, ). If indeed the bisabolene isomers share a similar mechanism of action, the specificity towards cancer cells that we observe may be because of differential HDAC2 activity in breast cancer cells.…”