Quantitative PCR to estimate copepod feeding

Nejstgaard, Jens C.; Frischer, Marc E.; Simonelli, Paolo; Troedsson, Christofer; Brakel, M.A.C. van den; Adiyaman, Filiz; Sazhin, Andrey F.; Artigas, Luis Felipe

doi:10.1007/s00227-007-0830-x

Cited by 102 publications

(96 citation statements)

References 82 publications

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“…However, the present study shows the importance of species-speciWc observations and the need of new methods to measure grazing. Ideally, it should be possible to directly quantify in situ ingestion of all prey of a single zooplankton by analysis of a freshly caught individual or its faeces, without any prior interference with the food environment (Nejstgaard et al 2003(Nejstgaard et al , 2008. The genetic approach, introduced by Nejstgaard and co-workers seems to be most promising, but is still in its infancy.…”

Section: Resultsmentioning

confidence: 99%

Copepods grazing on Coscinodiscus wailesii: a question of size?

Jansen

2008

Helgol Mar Res

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Grazing of female copepods, Acartia clausi and Temora longicornis, on the large centric diatom Coscinodiscus wailesii, was studied in the laboratory. While A. clausi females did not feed on C. wailesii, T. longicornis showed a skilful handling of these large algae. The cells were not completely ingested but copepods bit a small piece out of the silica wall and then ingested the cell content only. Faecal pellet analyses showed little remains of the silica walls, and most of the content could only be classiWed as undeWned matter. Therefore the general analyses of faecal pellets and gut content of copepods from the Weld must be interpreted with caution.

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Section: Resultsmentioning

confidence: 99%

Copepods grazing on Coscinodiscus wailesii: a question of size?

Jansen

2008

Helgol Mar Res

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“…As an alternative, studies using quantitative PCR have been proposed to quantify the abundance of DNA present in the extract (e.g., Bowles et al 2011). In aquatic systems, this has been a particularly active line of research recently, with examples including both samples from wildlife (e.g., Nejstgaard et al 2008;Murray et al 2011;Durbin et al 2012;Leal et al 2014a) and from data collected in Fig. 5.…”

Section: Quantifying Interactionsmentioning

confidence: 99%

The use of DNA barcodes in food web construction—terrestrial and aquatic ecologists unite!

Roslin

Majaneva

2016

Genome

104

103

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By depicting who eats whom, food webs offer descriptions of how groupings in nature (typically species or populations) are linked to each other. For asking questions on how food webs are built and work, we need descriptions of food webs at different levels of resolution. DNA techniques provide opportunities for highly resolved webs. In this paper, we offer an exposé of how DNA-based techniques, and DNA barcodes in particular, have recently been used to construct food web structure in both terrestrial and aquatic systems. We highlight how such techniques can be applied to simultaneously improve the taxonomic resolution of the nodes of the web (i.e., the species), and the links between them (i.e., who eats whom). We end by proposing how DNA barcodes and DNA information may allow new approaches to the construction of larger interaction webs, and overcome some hurdles to achieving adequate sample size. Most importantly, we propose that the joint adoption and development of these techniques may serve to unite approaches to food web studies in aquatic and terrestrial systems-revealing the extent to which food webs in these environments are structured similarly to or differently from each other, and how they are linked by dispersal.Key words: DNA barcodes, food webs, species delimitation, species identification, trophic links, ecological networks.Résumé : En brossant le tableau de qui mange qui, les réseaux trophiques livrent une description des liens qui unissent des groupes d'espèces ou de populations. Afin de déterminer comment ces réseaux sont constitués et comment ils fonctionnent, il nous faut une description de ces réseaux à différents niveaux de résolution. Les techniques de l'ADN permettent de produire des réseaux trophiques de grande résolution. Dans ce travail, les auteurs décrivent comment les techniques fondées sur l'ADN, en particulier les codes à barres de l'ADN, ont récemment été employées pour étudier la structure des réseaux trophiques chez des systèmes tant terrestres qu'aquatiques. Les auteurs soulignent comment ces techniques peuvent servir à simultanément améliorer la résolution taxonomique des noeuds d'un réseau (i.e. les espèces) ainsi que les relations entre eux (qui mange qui). Les auteurs terminent en proposant des moyens via lesquels les codes à barres et l'information tirée de l'ADN rendent possible de nouvelles approches en vue de la construction de plus grands réseaux d'interaction et permettent de surmonter des difficultés rencontrées dans l'acquisition d'échantillons de taille suffisante. Surtout, les auteurs proposent que l'adoption et le développement conjoints de ces techniques peut contribuer à unifier les approches employées dans l'étude des réseaux trophiques au sein des systèmes aquatiques et terrestres. Cela permettra de révéler l'étendue de la similarité ou de la dissimilarité dans la façon dont sont structurés les réseaux dans ces différents environnements et comment ils sont liés par la dispersion. [Traduit par la Rédaction]

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“…Mesozooplankton, and in particular copepods (Maxillopoda: Copepoda), rank among the most abundant metazoans in the ocean, both in terms of abundance and biomass (Nejstgaard et al 2008), according them link between primary production and higher trophic levels. Due to their small size, many phytoplankton species with diameters < 5 µm are not typically considered to be important food sources for copepods, which frequently exhibit prey choice based on size (Sommer et al 2000) or swimming behavior (Kiørboe & Visser 1999), and even chemical cues in the case of larger aggregates (Goncalves & Kiørboe 2015).…”

Section: Introductionmentioning

confidence: 99%