Quantitative Microdetermination of Enzymes in Sweat Gland

Quantitative Microdetermination of Enzymes in Sweat Gland: V. Ouabain-Sensitive Sodium-Potassium Activated Adenosinetriphosphatase

“…For isolation of sweat gland tissue [2], skin biopsies were taken with a high speed rotary drill of 3 mm diameter [9], using 1 % procaine as a local anesthetic. The plugs (6 to 10 from each patient) were imbedded in partially frozen tragacanth gel, frozen at -20°C, and sliced at 32 , u thickness with a cryostat microtome.…”

Quantitative Microdetermination of Enzymes in Sweat Gland: V. Ouabain-Sensitive Sodium-Potassium Activated Adenosinetriphosphatase

“…For isolation of sweat gland tissue [2], skin biopsies were taken with a high speed rotary drill of 3 mm diameter [9], using 1 % procaine as a local anesthetic. The plugs (6 to 10 from each patient) were imbedded in partially frozen tragacanth gel, frozen at -20°C, and sliced at 32 , u thickness with a cryostat microtome.…”

Quantitative Microdetermination of Enzymes in Sweat Gland: V. Ouabain-Sensitive Sodium-Potassium Activated Adenosinetriphosphatase

“…For isolation of sweat gland tissue [2], skin biopsies were taken with a high speed rotary drill of 3 mm diameter [9], using 1 % procaine as a local anesthetic. The plugs (6 to 10 from each patient) were imbedded in partially frozen tragacanth gel, frozen at -20°C, and sliced at 32 , u thickness with a cryostat microtome.…”

Quantitative Microdetermination of Enzymes in Sweat Gland: V. Ouabain-Sensitive Sodium-Potassium Activated Adenosinetriphosphatase

“…For isolation of sweat gland tissue [2], skin biopsies were taken with a high speed rotary drill of 3 mm diameter [9], using 1 % procaine as a local anesthetic. The plugs (6 to 10 from each patient) were imbedded in partially frozen tragacanth gel, frozen at -20°C, and sliced at 32 , u thickness with a cryostat microtome.…”