Quantitative MALDI MS Using Ionic Liquid Matrices

Tucher, Joanna; Somasundaram, Prasath; Tholey, Andreas

doi:10.1007/978-3-319-04819-2_5

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…The use of internal standard can be avoided when a linear correlation between analyte concentration and ion counts can be demonstrated [102]. A possible alternative to the utilization of an internal standard is the use of Ionic-Liquid Matrices [103].…”

Section: Quantitation Issuesmentioning

confidence: 99%

Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases

Preianò

Savino

Villella

et al. 2020

IJMS

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Given its intrinsic nature, gingival crevicular fluid (GCF) is an attractive source for the discovery of novel biomarkers of periodontal diseases. GCF contains antimicrobial peptides and small proteins which could play a role in specific immune-inflammatory responses to guarantee healthy gingival status and to prevent periodontal diseases. Presently, several proteomics studies have been performed leading to increased coverage of the GCF proteome, however fewer efforts have been done to explore its natural peptides. To fill such gap, this review provides an overview of the mass spectrometric platforms and experimental designs aimed at GCF peptidome profiling, including our own data and experiences gathered from over several years of matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) based approach in this field. These tools might be useful for capturing snapshots containing diagnostic clinical information on an individual and population scale, which may be used as a specific code not only for the diagnosis of the nature or the stage of the inflammatory process in periodontal disease, but more importantly, for its prognosis, which is still an unmet medical need. As a matter of fact, current peptidomics investigations suffer from a lack of standardized procedures, posing a serious problem for data interpretation. Descriptions of the efforts to address such concerns will be highlighted.

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Section: Quantitation Issuesmentioning

confidence: 99%

Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases

Preianò

Savino

Villella

et al. 2020

IJMS

View full text Add to dashboard Cite

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Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Harvey

2021

Mass Spectrometry Reviews

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This review is the ninth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.

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MALDI mass spectrometry in food carbohydrates analysis: A review of recent researches

et al. 2023

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Quantitative MALDI MS Using Ionic Liquid Matrices

Cited by 3 publications

References 35 publications

Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases

Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

MALDI mass spectrometry in food carbohydrates analysis: A review of recent researches

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