Quantitative Evaluation of Epstein-Barr-Virus-Infected Mononuclear Peripheral Blood Leukocytes in Infectious Mononucleosis

Rocchi, G; Felici, A; Ragona, Giuseppe; Heinz, A.

doi:10.1056/nejm197701202960302

Cited by 157 publications

(52 citation statements)

References 13 publications

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“…From these studies it would appear that the principal cell harbouring MHV-68 during the acute and latent infection is an Igpositive B lymphocyte. The mean number of infected cells in the B cell population (Table 1) was found to be approximately 1/105 spleen white cells, which is similar to the number reported in patients with infectious mononucleosis but more than that seen in B cells taken from peripheral blood during a chronic EBV infection (Rocchi et al, 1977).…”

supporting

confidence: 77%

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

Sunil-Chandra

Efstathiou

Nash

1992

Journal of General Virology

323

306

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supporting

confidence: 77%

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

Sunil-Chandra

Efstathiou

Nash

1992

Journal of General Virology

323

306

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“…The latter hypothesis may explain why RA lymphocytes spontaneously transform in culture more frequently than normal controls (20,22). During acute infectious mononucleosis, approximately 5% of B lymphocytes are transformed, but this falls to 1 per million within 3 months (23). If 5% of peripheral cells were transformed, we should be able to demonstrate RANA in these since we can detect 1 ce11/1,000.…”

Section: Discussionmentioning

confidence: 96%

A Search for Rheumatoid Arthritis‐Associated Nuclear Antigen And Epstein‐Barr Virus Specific Antigens or Genomes in Tissues and Cells from Patients with Rheumatoid Arthritis

Alspaugh

Shoji

Nonoyama³

1983

Arthritis & Rheumatism

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Pannus extracts andor cells and tissues from rheumatoid arthritis (RA) patients and controls were tested for the presence of RA-associated nuclear antigen (RANA), anti-RANA, Epstein-Barr virus (EBVbspecific antigens, and EBV genomes. Using our present rather insensitive methods, RANA was rarely detected in RA pannus extracts or tissues, whereas anti-RANA was commonly found. EBV-specific antigens or genomes could not be detected in a limited number of tissues tested using our present methods. The data suggest that EBV may not be the primary event causing RA through direct infection and transformation of synovial tissues.Previous studies have demonstrated that patients with rheumatoid arthritis (RA) have elevated levels of antibody directed toward an antigen termed RA-associated nuclear antigen (RANA) (1,2). Other studies showed that RANA was associated with Epstein-Barr virus (EBV) infection (3). More recent

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“…11 The number of infected B cells in healthy individuals has been estimated at 1 in 10 6 . 11,24 In our study, DNA is isolated from less than 0.5 ϫ 10 5 cells, and thus the levels of EBV in healthy individuals was probably less than the limits of detection in our assay. 11 What is the significance of detecting EBV PCR sequences in the blood of asymptomatic recipients?…”

Section: Discussionmentioning

confidence: 82%

Significance of detecting epstein-barr-specific sequences in the peripheral blood of asymptomatic pediatric liver transplant recipients

et al. 2000

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Pediatric allograft recipients are at increased risk for Epstein-Barr virus (EBV)-associated illnesses. The early identification and diagnosis of EBV-associated disorders is critical because disease progression can often be curtailed by modification of immunosuppression. We have previously shown that detection of EBV-specific sequences in the circulation by polymerase chain reaction (PCR) correlated well with the clinical symptoms of EBV infection. The purpose of the current study is to determine the significance of detecting EBV-specific sequences by PCR in asymptomatic pediatric liver transplant recipients. Peripheral-blood DNA was analyzed for the EBV genes, coding from the nuclear antigen 1 (EBNA-1) and the viral capsid antigen (gp220) by PCR. Samples from asymptomatic pediatric liver transplant recipients were analyzed from the immediate postoperative period and at 2-to 4-month intervals thereafter. We followed up 13 of these asymptomatic recipients who tested positive for EBV compared with 7 asymptomatic recipients who tested negative for EBV during the early posttransplantation period. Follow-up ranged from 1.5 to 4 years posttransplantation. Nine patients (69%) initially positive for EBV and asymptomatic ultimately developed symptoms of EBV infection, including fever, lymphadenopathy, rash, respiratory and gastrointestinal symptoms, and/or hepatitis. Five of these patients (56%) went on to develop posttransplant lymphoproliferative disorder based on histological examination of biopsied tissue and immunohistochemical identification of the EBV antigen/DNA in tissue. This is the first report suggesting that detection of EBV-specific sequences in the absence of symptoms may herald impending EBV-associated disorders. Thus, routine monitoring for circulating EBV sequences in asymptomatic recipients may be useful in the early identification of those at risk for developing EBV-associated disease and its ultimate prevention.

show abstract

Quantitative Evaluation of Epstein-Barr-Virus-Infected Mononuclear Peripheral Blood Leukocytes in Infectious Mononucleosis

Cited by 157 publications

References 13 publications

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

A Search for Rheumatoid Arthritis‐Associated Nuclear Antigen And Epstein‐Barr Virus Specific Antigens or Genomes in Tissues and Cells from Patients with Rheumatoid Arthritis

Significance of detecting epstein-barr-specific sequences in the peripheral blood of asymptomatic pediatric liver transplant recipients

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