Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis

Zhang, Xue; Zhang, Chong; Zhou, Qianqian; Zhang, Xiaofei; Wang, Liyan; Chang, Hai-Bo; Li, Heping; Oda, Yoshimitsu; Xing, Xin‐Hui

doi:10.1007/s00253-015-6678-y

Cited by 109 publications

(79 citation statements)

References 51 publications

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“…In particular, the research of CAP as a potential oncotherapeutic approach has thrived over the past decade and the mechanism is been increasingly understood [12][13][14][15][16]. It is widely reported that CAP deactivated more than 20 types of cancer in vitro by inducing apoptosis [17][18][19], cell cycle arrest [20][21][22], endoplasmic reticulum stress [23,24] and DNA damage [25][26][27]. CAP has also been shown to significantly reduce tumor volume in an in vivo murine model following s phase cell cycle arrest and apoptosis [28].…”

Section: Introductionmentioning

confidence: 99%

Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results

Cheng¹,

Rowe²,

Ly³

et al. 2018

Plasma

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Triple-negative breast cancer is a phenotype of breast cancer where the expression level of estrogen, progesterone and human epidermal growth factor receptor 2 (HER2) receptors are low or absent. It is more frequently diagnosed in younger and premenopausal women, among which African and Hispanic have a higher rate. Cold atmospheric plasma has revealed its promising ant-cancer capacity over the past two decades. In this study, we report the first cold plasma jet delivered by the Canady Cold Plasma Conversion Unit and characterization of its electric and thermal parameters. The unit effectively reduced the viability of triple-negative breast cancer up to 80% without thermal damage, providing a starting point for future clinical trials.

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Section: Introductionmentioning

confidence: 99%

Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results

Cheng¹,

Rowe²,

Ly³

et al. 2018

Plasma

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“…To obtain mutants with resistance to DPA and/or an analogue, the wild-type strains were treated by the conventional mutation methods, such as ultraviolet (UV) and N-methyl-N-nitro-N-nitroso-guanidine (NTG) (Tani et al, 1985;Sato et al, 2001a;2001b). Compared with these mutation methods, however, an atmospheric and room temperature plasma (ARTP) biological breeding system (Wuxi Tmaxtree Biotechnology Co., Ltd., Wuxi, China) has many outstanding features, such as high positive mutation rate, rapid mutation, and high operation flexibility 2015). For an ARTP breeding system, the DNA of microorganism is destroyed by different chemical reactive species (e.g.…”

Section: Introductionmentioning

confidence: 99%

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

Zhang

2017

J. Zhejiang Univ. Sci. B

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A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 high-producing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved ((30.2±2.7) mg/L). In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached (61.3±5.2) mg/L and 2.59 mg/L per OD unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.

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“…We have further developed genetically engineered umu test systems expressing human phase I drug metabolic enzyme (cytochrome P450) [8] and rat or human phase II drug enzymes (glutathione S -transferase, N -acetyltransfearses, and sulfotransferases) for determination of bioactivation of chemical procarcinogens and promutagens and studies of mechanisms of genotoxicity or carcinogenesis [9–11]. Finally, we recently published our papers on the application of umu test to photogenotoxicity [12] and flow cytometry analysis [13]. …”

Section: Introductionmentioning

confidence: 99%

“…However, ARTP mutagenesis has not been quantitatively compared with conventional mutation methods. Recently, we developed umu test using a flow-cytometric analysis to quantify the DNA damage caused by ARTP, UV, and chemical (4-NQO and MNNG) treatments in individual viable cells using S. typhimurium NM2009 as the model strain and to determine the mutation rate [13]. The mutation rate was found to be proportional to the corresponding SOS response induced by DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Development and progress for three decades in umu test systems

Oda

2016

Genes and Environ

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Umu test have been widely used to predict the detection and assessment of DNA- damaging chemicals in environmental genotoxicity field for three decades. This test system is more useful with respect to simplicity, sensitivity, rapidity, and reproducibility. A review of the literature on the development of the umu test is presented in this article. The contents of this article are included a description of numerous data using the umu test. This test have been fully evaluated and used in many directions. Different genetically engineered umu systems introducing bacterial and rat or human drug metabolizing enzymes into the umu tester strains, have been successfully established and are considered as useful tools for genotoxicity assays to study the mechanisms of biotransformation in chemical carcinogenesis. Actually, we developed that two types of bacterial metabolizing enzymes and 4 types of rat and human metabolizing enzyme DNAs are expressed in these strains such as nitroreductase and O-acetyltransferase, cytochrome P450, N-acetyltransferases, sulfotransferases, and glutathione S-transferases, respectively. Due to increasing numbers of minute environmental samples and new pharmaceuticals, a high-throughput umu test system using Salmonella typhimurium TA1535/pSK1002, NM2009, and NM3009 strains provides a useful for these genotoxicity screening. I also briefly describe the first attempts to incorporate such umu tester strain into photo-genotoxicity test.

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Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis

Cited by 109 publications

References 51 publications

Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results

Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

Development and progress for three decades in umu test systems

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