Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of <i>Toddalia asiatica</i> (Linn.) Using HPTLC

Abstract

Toddalia asiatica Linn. is an important medicinal plant belonging to the family Rutaceae. The plant is well known for its antimalarial activity, which has been attributed to the presence of benzophenanthridine alkaloid nitidine in the roots of plants. A simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been developed and validated for the quantitative determination of nitidine in the dried roots and plant tissue culture extracts of T. asiatica. Nitidine was estimated at 332 nm by densitometry using Silica gel 60 F 254 as stationary phase and chloroform:methanol (7:1, v/v), and as mobile phase. Linearity was observed in the concentration range of 25 -200 ng/spot for nitidine. The limit of detection and limit of quantitation were found to be 0.026 and 0.086 ng/spot respectively for nitidine. Developed method was validated according to the ICH guidelines with respect to precision, accuracy, specificity and robustness. The technique has been applied for the first time for the estimation of nitidine in roots and plant tissue culture extracts of T. asiatica. Statistical analysis data indicate the accuracy and reliability of the method.

Prospect of Natural Compounds Against Malaria: A Review

“…3C) and this compound was tested against different strains of falciparum. 40 It performs its action in a similar fashion to chloroquine by inhibiting the formation of β-haematin.…”

Simultaneous Quantitative Determination of Nitidine, Chelerythrine and Sanguinarine Using HPTLC from Callus Extract of <i>Zanthoxylum rhetsa</i>

“…The peaks corresponding to nitidine, chelerythrine and sanguinarine from the sample solutions had the same retention factor as that of three standard drugs. Praveena and Veeresham in 2014 and 2015 [19] [20] reported that the nitidine has R f value 0.28 from the roots and tissue culture extracts of Toddalia asiatica. Similarly Bogucka and Zalewski in 2017 [21] reported that the R f values of chelerythine (0.35) and sanguinarine (0.45) from roots and herbs of Chelidonium majus.…”

A Simple and Efficient Two-Dimensional High-Speed Counter-Current Chromatography Linear Gradient and Isocratic Elution Modes for the Preparative Separation of Coumarins from Roots of Toddalia asiatica (Linn.) Lam.

“…Previous studies show that different methods, such as GC/MS [ 3 , 13 , 14 , 15 ], HPLC [ 16 , 17 , 18 ], UPLC-MS/MS [ 19 ] UPLC-QTOF-MS/MS [ 17 ], and HPTLC [ 20 ], can be used for the purification of the chemical constitutes in T. asiatica . High-speed counter-current chromatography (HSCCC) using the n -hexane/ethyl acetate/methanol/water (10:10:11:9, v / v ) solvent system is reported for the separation of isopimpinellin, pimpinellin, and phellopterin from T. asiatica [ 21 ].…”