Quantitative detection of aflatoxin B1 using quantum dots-based immunoassay in a recyclable gravity-driven microfluidic chip

Merging microfluidics with luminescence immunoassays for urgent point-of-care diagnostics of COVID-19

“…The speed can be adjusted by the size of corresponding capillary pumps. Besides microstructures serving as power for self-driven, gravity and suction are also applied for fluid control [ 152 , 278 , 279 ]. As shown in Fig.…”

“…As shown in Fig. 10 A, Xiang et al present a recyclable gravity-driven LOCC system for competitive immunoassays [ 278 ]. In the alternative, finger-driven microfluidic chips have attracted wide attention, eliminating the need for any skilled personnel with simple design and operation [ 35 , [280] , [281] , [282] ].…”

Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification

“…At present, the market products available for the detection of aflatoxin focus mainly on chromatography-based ( Er Demirhan and Demirhan, 2022 , Wu et al, 2021 , Sarwat et al, 2022 , Xuan et al, 2019 ) and immunoassay-based ( Li et al, 2021 , Yan et al, 2022 , Wang et al, 2021 ) solutions( Li et al, 2021 , Wang et al, 2021 , Yan et al, 2022 ). Although chromatography is highly sensitive and exhibits a good reproducibility, its detection cost is high, it requires large-scale instruments and professional operators, and the sample pretreatment process is relatively complicated ( Lerdsri et al, 2021 , Xiang et al, 2021 ). Therefore, this approach is unsuitable for on-site testing.…”

“…Therefore, this approach is unsuitable for on-site testing. In addition, although the immunoassay-based approach offers some advantages over chromatography ( Lerdsri et al, 2021 , Xiang et al, 2021 ), antibodies are required as recognition elements, which presents new challenges. For example, antibody-based approaches are expensive, a poor thermal stability, and difficulty in terms of the transportation and storage, thereby hindering the development and application of immunological technologies ( Ni, Zhuo, Pan, Yu, Li, Liu, et al, 2020 ).…”

Quantum-Dot-Bead-Based Fluorescence-Linked Immunosorbent Assay for Sensitive Detection of Cry2A Toxin in Cereals Using Nanobodies

“…Besides, the sensitivity of immunoassays is greatly affected by the signal probes. To date, many fluorescent probes (e.g., quantum dots (QDs), up-conversion nanoparticles, and lanthanide ions) instead of traditional enzymes have been applied to enhance the sensitivity of immunoassays [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. Among them, QDs are ideal fluorescent materials because of their narrow emission spectra, broad excitation, high fluorescent intensity, and high stability [ 36 , 37 ].…”