Quantitative changes of the alkaloid complex in a submerged culture ofClaviceps paspali

Abstract

Claviceps paspali FA produced high concentrations of alkaloid under submerged conditions. Their production was found to depend on the developmental stage and treatment of the filamentous culture inoculum. A medium containing Bacto-peptone with a constant composition of amino acids was selected for the preparation of the inoculum. A two-week fermentation in a synthetic medium with mannitol at 24 +/- 1 degrees C resulted in an increased production of total alkaloids from the original value of 100-200 micrograms/mL to more than 2000 micrograms/mL. Addition of tryptophan did not further increase the production of alkaloids but resulted in changes of the spectrum of some metabolites, 2,3-Dihydroxybenzoic acid accompanied the alkaloids in the fermentation medium. alpha-Hydroxyethyllysergamide was the predominant component of extracellular alkaloids (80% in the first days of fermentation). During fermentation the level of this alkaloid continuously decreased while the concentration of the accompanying alkaloids, i.e. lysergamide and the corresponding minor isomers, increased.

Inactivation of the indole-diterpene biosynthetic gene cluster of Claviceps paspali by Agrobacterium-mediated gene replacement

“…C. paspali has long been used in the pharmaceutical industry to produce lysergic acid and similar ergot alkaloids in submerged liquid cultures (Ricicová et al 1982; Socic et al 1986). Nevertheless, the targeted molecular genetic modification of this fungus remained a major challenge due to the low efficiencies of most steps in the transformation workflow, including protoplast regeneration, DNA introduction, mitotically stable integration of the incoming DNA, and homogenization of the transformant allele.…”

New Alkaloids of Claviceps paspali

“…A mechanism of formation of these compounds is proposed. The C-8 configuration of 8-hydroxyergotamine was solved by comparison with nmr spectra of 1 and 2.Submerged fermentation of various strains of Claviceps paspali Stevens et Hall is a long-studied subject (1)(2)(3)(4)(5)(6). Only a few authors (1,2,6) have mentioned a decline in the alkaloid content in the later production phase.…”

“…Submerged fermentation of various strains of Claviceps paspali Stevens et Hall is a long-studied subject (1)(2)(3)(4)(5)(6). Only a few authors (1,2,6) have mentioned a decline in the alkaloid content in the later production phase.…”