Quantitative Bioanalysis in Drug Discovery and Development: Principles and Applications

El‐Kattan, Ayman; Holliman, Chris; Cohen, Lee M.

doi:10.1002/9780470409817.ch2

Cited by 4 publications

(2 citation statements)

References 49 publications

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“…The off-line methods all provided appropriate levels of sensitivity for the intended purpose. This reflects the improvement in mass spectrometer sensitivity through technological advances in recent years which has meant that in general, pharmacokinetic analysis in drug discovery and development can be readily achieved using available technologies [9].…”

Section: Discussionmentioning

confidence: 99%

The application of fully automated on-line solid phase extraction in bioanalysis

Mitchell

Christian

Hughes

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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Section: Discussionmentioning

confidence: 99%

The application of fully automated on-line solid phase extraction in bioanalysis

Mitchell

Christian

Hughes

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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“…In order to overcome this resistance, the combination of ceftazidime with the β-lactamase inhibitor avibactam is currently being developed. 4 It is accepted that stable isotopically labeled compounds are the 'gold standard' 5,6 internal standards for use in quantitative bioanalysis and can be more successful in correcting for matrix effects in mass spectrometry assays than structural analogs. 7 For this reason, stable isotope-labeled ceftazidime was required to support ongoing clinical studies.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of [²H₆]ceftazidime as a stable isotopically labeled internal standard

Bian¹,

Bushby

2015

Labelled Comp Radiopharmac

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Ceftazidime is a third generation cephalosporin antibiotic that has activity against a wide range of Gram-negative and Gram-positive bacterial pathogens, including Escherichia coli and Pseudomonas aeruginosa. Stable isotope-labeled ceftazidime was required for use as an internal standard in LC-MS/MS assays, and a route was developed to make [(2)H6]ceftazidime in eight steps from the commercially available labeled starting material [(2)H7]isobutyric acid.

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Hybrid Mass Analyzers in Drug Metabolism Applications

Chacko

Ramanathan

Humphreys

et al. 2012

Encyclopedia of Drug Metabolism and Interactions

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Among the analytical techniques used in the biopharmaceutical industry, liquid chromatography mass spectrometry (LC‐MS) assays are highly sought out by the drug metabolism and pharmacokinetic (DMPK) scientists due to their sensitivity, selectivity, specificity, and speed. Some of the desired qualities of the individual mass analyzers (quadrupole mass filters, ion traps and time‐of‐flights) and ion manipulation techniques are combined into one instrument to configure hybrid mass spectrometers. Such hybrid mass spectrometers become even more selective and specific when either time‐of‐flight or Fourier transform based high resolution mass spectrometers (HRMS) are combined with either ion traps or quadrupole mass filters. HRMS‐based hybrid mass analyzers, including quadrupole‐ time‐of‐flights, linear ion trap‐orbitrap, and quadrupole‐orbitrap, have begun to become front line mass spectrometers across the biopharmaceutical industry, especially in the DMPK laboratories. The focus of this book chapter is hybrid mass spectrometers based strategies and procedures available for detection and characterization of xenobiotics at various stages of drug discovery and development.

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Quantitative Bioanalysis in Drug Discovery and Development: Principles and Applications

Cited by 4 publications

References 49 publications

The application of fully automated on-line solid phase extraction in bioanalysis

The application of fully automated on-line solid phase extraction in bioanalysis

Synthesis of [²H₆]ceftazidime as a stable isotopically labeled internal standard

Hybrid Mass Analyzers in Drug Metabolism Applications

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Quantitative Bioanalysis in Drug Discovery and Development: Principles and Applications

Cited by 4 publications

References 49 publications

The application of fully automated on-line solid phase extraction in bioanalysis

The application of fully automated on-line solid phase extraction in bioanalysis

Synthesis of [2H6]ceftazidime as a stable isotopically labeled internal standard

Hybrid Mass Analyzers in Drug Metabolism Applications

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Product

Resources

About

Synthesis of [²H₆]ceftazidime as a stable isotopically labeled internal standard