Quantitative and qualitative analysis of the effectiveness of a mechanical method for the isolation of preantral follicles from ovine ovaries

Amorim, Christiani Andrade; Lucci, Carolina Madeira; Rodrigues, Ana Paula Ribeiro; Carvalho, Fabíola Christian Almeida de; Figueiredo, José Ricardo de; Rondina, Davide; Cecchi, R.; Giorgetti, Alessandro; Martini, Andrea; Gonçalves, Paulo Bayad Dias

doi:10.1016/s0093-691x(00)00269-7

Cited by 51 publications

(32 citation statements)

References 34 publications

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“…This effect has already been demonstrated in other species, like caprine [6] and ovine [5]. In the present work, the greatest number of isolated Zebu cow preantral follicles was obtained using the 125 pm interval.…”

Section: Discussionsupporting

confidence: 83%

“…In the present work, the greatest number of isolated Zebu cow preantral follicles was obtained using the 125 pm interval. In goats and sheep, the best results were obtained with the intervals of 75 pm [6], and 87.5 pm [5], respectively. These results suggest, again, that the most suitable interval for sectioning the ovarian tissue using a tissue chopper varies among species.…”

Section: Discussionmentioning

confidence: 97%

“…Other researchers isolated a large number of ovarian preantral follicles from European cows [3,15,24], goats [4,6], ewes [2,5], pigs [25], domestic cats [26,27] and nondomestic felids [28,29], rabbits [30], rats [31], mice [32], and humans [33], using mechanical [2^1, 15,24,[26][27][28][29] or enzymatic [25,[30][31][32][33] procedures. Comparisons between our results and those of other studies are difficult to perform due to species differences, variation in follicle classification, and types of follicles included in the final count.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, one of the most efficient methods for isolating a large number of follicles consists of using a tissue chopper [2][3][4]. Recently, however, studies have demonstrated that the best interval for sectioning the ovarian tissue using the tissue chopper can vary according to species [5,6]. In addition to isolating a large number of follicles, a suitable method should preserve the normal follicular morphology after isolation, not only on the structural level, but also on the ultrastructural level [7].…”

Section: Introductionmentioning

confidence: 99%

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation methodCarolina Madeira Lucci Rodolfo Rumpf José Ricardo Figueiredo Sonia Nair Béo AbstractPreantral follicles are a major source of oocytes, and their utilization as an important tool to store large number of female gametes for future use in reproductive programs has been investigated. The increasing importance of studies in this subject, together with the important role of Zebu cattle in the economy of tropical and subtropical countries as well as their well-known differences from European cattle, led to this research. The present study aims to determine the best size interval for sectioning ovarian tissue to isolate preantral follicles from Zebu cows using a tissue chopper and to evaluate the follicular quality after isolation. Furthermore, it aims to provide information about the Zebu cow preantral follicle population and use this data (as a control) to evaluate the effectiveness of the tested isolation method. Testing eight different tissue sectioning size intervals, it was possible to conclude that the 125-pm-section interval is shown to be better than the intervals of 25, 50, 175 and 200 pm to isolate preantral follicles from Zebu cow ovaries. The 125-pm interval allowed the recovery of 26,050±1611 (meaniS.E.M.) preantral follicles per one-half ovary, while the number of preantral follicles in situ estimated by evaluation of histological sections was 35,28812342 per one-half ovary. Thus, the mean (iS.E.M.) recovery rate (=[number of preantral follicles isolated/number of preantral follicles in situ in the same ovary] x 100) was 74.3±4.3%. The morphometrical analysis showed that Bos indicus preantral follicles are similar to B. taurus preantral follicles based on previous reports. In conclusion, this study showed that a simple, mechanical method can be used effectively to isolate a large number of intact preantral follicles from Zebu cow ovaries, with a high recovery rate.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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“…In caprine and ovine studies of estimation of follicular population in ovaries, 6 and 0.1% of degenerated preantral follicles were observed, respectively (Lucci et al, 1999 andAmorim et al, 2000). In contrast, the degeneration rate of preantral follicles was quoted as being 65% in feline ovary (Wood et al, 1997).…”

Section: Discussionmentioning

confidence: 96%