Quantitative Analysis of Optic Disc Color

Abstract

PurposeTo evaluate the reproducibility of ImageJ software in analyzing the color of the optic disc.MethodsOne hundred twelve normal participants (56 males and 56 females) were enrolled in this study. The image of the optic disc was taken using Kowa digital disc photo-graphy, and the gray scales of the nasal rim (NR), brightest cupping center (BCC) and largest inferior retinal vein (IRV) were calculated using histogram in ImageJ. Three different observers calculated the gray scales three separate times. Reproducibility was assessed using the interclass correlation coefficient (ICC).ResultsThe mean age of the participants was 50.6 years old (range, 11 to 82 years). The mean gray scales of the nasal rim were 91.81, 94.91, and 93.24; those of the brightest cupping center were 174.84, 179.94, and 177.76; and those of the largest inferior retinal vein were 61.85, 53.48, and 56.73 for observers 1, 2, and 3, respectively. Inter-observer reproducibility for NR, BCC and IRV was considered good based upon ICC values of 0.944, 0.860, and 0.789 for observers 1, 2, and 3, respectively. Significant age-related differences between the values of the brightest cupping center were noted, and the gray scale score was decreased in the older participants (p < 0.001).ConclusionsThe gray scale of the brightest cupping center diminished with age. ImageJ can be a useful objective tool with high reproducibility in the analysis of optic disc color.

Using ImageJ to Evaluate Optic Disc Pallor in Traumatic Optic Neuropathy

“…Our previous study showed that ImageJ is a useful, objective tool for optic disc color analysis and that it has a high inter- and intra-observer reproducibility [ 9 ]. In this report, the gray scale value of the largest inferior retinal vein had a smaller standard deviation than the nasal rim and the brightest cupping center.…”

“…We previously demonstrated quantitative optic disc pallor analysis using fundus photography and free image analysis software, ImageJ (US National Institutes of Health, Bethesda, MD, USA). These readily available tools were used to obtain highly reproducible, objective evaluations of optic disc color [ 9 ]. The purpose of the current study was to investigate whether optic disc pallor, objectively quantified by Image J software, is correlated with OCT-measured RNFL loss.…”

Using ImageJ to Evaluate Optic Disc Pallor in Traumatic Optic Neuropathy

“…Our previous study showed that ImageJ is a useful, objective tool for optic disc color analysis and that it has a high inter- and intra-observer reproducibility [ 9 ]. In this report, the gray scale value of the largest inferior retinal vein had a smaller standard deviation than the nasal rim and the brightest cupping center.…”

“…We previously demonstrated quantitative optic disc pallor analysis using fundus photography and free image analysis software, ImageJ (US National Institutes of Health, Bethesda, MD, USA). These readily available tools were used to obtain highly reproducible, objective evaluations of optic disc color [ 9 ]. The purpose of the current study was to investigate whether optic disc pallor, objectively quantified by Image J software, is correlated with OCT-measured RNFL loss.…”

Letter to the Editor: Quantitative Analysis of Optic Disc Color

“…I read with great interest the article by Kim et al [ 1 ] regarding the use of ImageJ to quantitatively analyze the color of optic discs. The authors proposed three points to measure the intensity per pixel area after grayscale conversion, namely nasal rim (NR), brightest cupping center (BCC), and inferior retinal vein (IRV).…”

Quantitative method for in vitro matrigel invasiveness measurement through image analysis software

“…ImageJ is already available as freeware and is widely used in several scientific fields [15]. The use of ImageJ and other image analysis programs can be found elsewhere, including area calculation, particle counting, shape and size analysis, dot blot analysis, color analysis, and other applications [15][16][17][18][19][20][21]. Using the ''area method'', described by Girish et al [22], the total area occupied by stained cells can be selected using ImageJ's thresholding tool.…”

Using ImageJ to Evaluate Optic Disc Pallor in Traumatic Optic Neuropathy

“…Our previous study showed that ImageJ is a useful, objective tool for optic disc color analysis and that it has a high inter- and intra-observer reproducibility [ 9 ]. In this report, the gray scale value of the largest inferior retinal vein had a smaller standard deviation than the nasal rim and the brightest cupping center.…”

“…We previously demonstrated quantitative optic disc pallor analysis using fundus photography and free image analysis software, ImageJ (US National Institutes of Health, Bethesda, MD, USA). These readily available tools were used to obtain highly reproducible, objective evaluations of optic disc color [ 9 ]. The purpose of the current study was to investigate whether optic disc pallor, objectively quantified by Image J software, is correlated with OCT-measured RNFL loss.…”

Letter to the Editor: Quantitative Analysis of Optic Disc Color

“…I read with great interest the article by Kim et al [ 1 ] regarding the use of ImageJ to quantitatively analyze the color of optic discs. The authors proposed three points to measure the intensity per pixel area after grayscale conversion, namely nasal rim (NR), brightest cupping center (BCC), and inferior retinal vein (IRV).…”

Quantitative method for in vitro matrigel invasiveness measurement through image analysis software

“…ImageJ is already available as freeware and is widely used in several scientific fields [15]. The use of ImageJ and other image analysis programs can be found elsewhere, including area calculation, particle counting, shape and size analysis, dot blot analysis, color analysis, and other applications [15][16][17][18][19][20][21]. Using the ''area method'', described by Girish et al [22], the total area occupied by stained cells can be selected using ImageJ's thresholding tool.…”

Using ImageJ to Evaluate Optic Disc Pallor in Traumatic Optic Neuropathy

“…Our previous study showed that ImageJ is a useful, objective tool for optic disc color analysis and that it has a high inter- and intra-observer reproducibility [ 9 ]. In this report, the gray scale value of the largest inferior retinal vein had a smaller standard deviation than the nasal rim and the brightest cupping center.…”

“…We previously demonstrated quantitative optic disc pallor analysis using fundus photography and free image analysis software, ImageJ (US National Institutes of Health, Bethesda, MD, USA). These readily available tools were used to obtain highly reproducible, objective evaluations of optic disc color [ 9 ]. The purpose of the current study was to investigate whether optic disc pallor, objectively quantified by Image J software, is correlated with OCT-measured RNFL loss.…”

Letter to the Editor: Quantitative Analysis of Optic Disc Color

“…I read with great interest the article by Kim et al [ 1 ] regarding the use of ImageJ to quantitatively analyze the color of optic discs. The authors proposed three points to measure the intensity per pixel area after grayscale conversion, namely nasal rim (NR), brightest cupping center (BCC), and inferior retinal vein (IRV).…”

Quantitative method for in vitro matrigel invasiveness measurement through image analysis software

“…ImageJ is already available as freeware and is widely used in several scientific fields [15]. The use of ImageJ and other image analysis programs can be found elsewhere, including area calculation, particle counting, shape and size analysis, dot blot analysis, color analysis, and other applications [15][16][17][18][19][20][21]. Using the ''area method'', described by Girish et al [22], the total area occupied by stained cells can be selected using ImageJ's thresholding tool.…”