Quantitative analysis of cell types during growth, degrowth and regeneration in the planarians Dugesia mediterranea and Dugesia tigrina

Baguñà, Jaume; Romero, Rafael

doi:10.1007/978-94-009-8668-8_25

Cited by 87 publications

(134 citation statements)

References 9 publications

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“…Although the planarian brain has long been considered as one of the "simplest" brains within the Bilateria (Keenan et al, 1981), it is now becoming evident that the planarian CNS and in particular the brain actually shows a complex cytoarchitecture that is functionally regionalized allowing the performance of complex processes (Cebrià et al, 2002;Nakazawa et al, 2003;Cebrià et al, 2007). A brain capable of complex responses to the environment within the context of an organism that is continuously remodelling its body (Morgan, 1898) including the CNS (Baguñà and Romero, 1981) must require a very precise regulation of synaptic development and plasticity. miRNAs may play an important role in this process, and the variety of miRNAs expressed in and in close proximity to the CNS supports this.…”

Section: Discussionmentioning

confidence: 99%

Diverse miRNA spatial expression patterns suggest important roles in homeostasis and regeneration in planarians

González‐Estévez¹,

Arseni²,

Thambyrajah³

et al. 2009

Int. J. Dev. Biol.

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miRNAs are an important class of non-protein coding small RNAs whose specific functions in animals are rapidly being elucidated. It is clear that miRNAs can play crucial roles in stem cell maintenance, cell fate determination and differentiation. We use planarians, which possess a large population of pluripotent somatic stem cells, as a powerful model system to study many aspects of stem cell biology and regeneration. In particular we wish to investigate the regulatory role miRNAs may have in planarian stem cell self renewal, proliferation and differentiation. Here, we characterized the differential spatial patterns of expression of miRNAs in whole and regenerating planarians by in situ hybridization to nascent miRNA transcripts. These miRNA expression patterns are the first which have been determined for a Lophotrocozoan animal. We have characterized the expression patterns of 42 miRNAs in adult planarians, constituting a complete range of tissue specific expression patterns. We also followed miRNA expression during planarian regeneration. The majority of planarian miRNAs were expressed either in areas where stem cells (neoblasts) are located and/or in the nervous system. Some miRNAs were definitively expressed in stem cells and dividing cells as confirmed by in situ hybridisation after irradiation. We also found miRNAs to be expressed in germ stem cells of the sexual strain. Together, these data suggest an important role for miRNAs in stem cell regulation and in neural cell differentiation in planarians.

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Section: Discussionmentioning

confidence: 99%

Diverse miRNA spatial expression patterns suggest important roles in homeostasis and regeneration in planarians

González‐Estévez¹,

Arseni²,

Thambyrajah³

et al. 2009

Int. J. Dev. Biol.

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“…21 We suggest that at the beginning of starvation, resources for continued proliferation will come from food present in the gastrodermal cells and from reserves present in the parenchyma cells. After their depletion, non-essential cells (such as the cells of the sexual organs) undergo autophagy.…”

Section: Autophagy Enters the Game: A New Possible Scenario For Morphmentioning

confidence: 93%

Gtdap-1and the Role of Autophagy During Planarian Regeneration and Starvation

González‐Estévez¹,

Felix²,

Aboobaker³

et al. 2007

Autophagy

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“…We postulate that this expression can be associated with determination or commitment of these cells to intestinal cells. Indeed, gastrodermal cells are short-lived cells (Baguñà and Romero 1981) and are continuously being replaced by differentiating neoblasts, as have been extensively documented by light and electron microscopy (Hori 1986). HSP90 is one of the most abundant proteins in the cytosol of eukaryotic cells even in the absence of stress, being required for final maturation of different substrates (Csermely et al 1998;Nathan et al 1997), and its distribution pattern (ubiquitary or tissue-specific) varies in different organisms (Krone and Sass 1994;Li et al 2009a, b).…”

Section: Characterization Of Djhsp90mentioning

confidence: 99%

Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians

Conte

Isolani

Deri

et al. 2011

Cell Stress and Chaperones

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Heat shock proteins (HSPs) play a crucial role in the protection of cells. In the present study, we have identified an hsp90-related gene (Djhsp90) encoding a cytosolic form of HSP90 that is primarily expressed in gastrodermis of the planarian Dugesia japonica. Djhsp90 becomes significantly induced after traumatic amputation or other stress stimuli, such as exposure to X-ray or ultraviolet radiations, heat shock, or prolonged starvation. When Djhsp90 is silenced by ribonucleic acid interference (RNAi), planarians dramatically decrease in size, becoming unable to eat, and die in a few weeks. Our results indicate that this gene plays an essential cytoprotective role in the gastrodermis of planarians and suggest that this chaperone can be involved in autophagic processes that are activated by this tissue.

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Quantitative analysis of cell types during growth, degrowth and regeneration in the planarians Dugesia mediterranea and Dugesia tigrina

Cited by 87 publications

References 9 publications

Diverse miRNA spatial expression patterns suggest important roles in homeostasis and regeneration in planarians

Diverse miRNA spatial expression patterns suggest important roles in homeostasis and regeneration in planarians

Gtdap-1and the Role of Autophagy During Planarian Regeneration and Starvation

Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians

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