Quantitative analysis of biofilm formation of methicillin-resistant<i>Staphylococcus aureus</i>(MRSA) strains from patients with orthopaedic device-related infections

Kawamura, Hideki; Nishi, Junichiro; Imuta, Naoko; Tokuda, Koichi; Miyanohara, Hiroaki; Hashiguchi, Teruto; Zenmyo, Michihisa; Yamamoto, Takuya; Ijiri, Kosei; Kawano, Yoshifumi; Komiya, Setsuro

doi:10.1111/j.1574-695x.2011.00821.x

Cited by 36 publications

(24 citation statements)

References 36 publications

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“…A survey of biofi lm production among 26 staphylococcal isolates (13 S. aureus , 10 S. epidermidis , 1 S. hominis , 1 S. warneri , 1 S. lugdunensis ) recovered from 23 orthopedic devices noted that all the strains formed biofi lms and 19 (73.1 %) possessed the ica gene (Esteban et al 2010 ). Biofi lm production was also surveyed among 168 MRSA isolates (23 device-associated, 55 nondevice associated and 90 nasal carriage isolates) from 87 infected and 95 colonized outpatients (Kawamura et al 2011 ). This study found a greater intensity of biofi lm staining among isolates from orthopedic devices.…”

Section: Biofi Lms From Aerobic Grampositive and Gram-negative Bacteriamentioning

confidence: 99%

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Akers

Cardile

Wenke

et al. 2014

Advances in Experimental Medicine and Biology

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Reports of biofilms have increased exponentially in the scientific literature over the past two decades, yet the vast majority of these are basic science investigations with limited clinical relevance. Biofilm studies involving clinical isolates are most often surveys of isolate collections, but suffer from lack of standardization in methodologies for producing and assessing biofilms. In contrast, more informative clinical studies correlating biofilm formation to patient data have infrequently been reported. In this chapter, biofilm surveys of clinical isolates of aerobic and anaerobic bacteria, mycobacteria, and Candida are reviewed, as well as those pertaining to the unique situation of cystic fibrosis. In addition, the influence of host components on in vitro biofilm formation, as well as published studies documenting the clinical impact of biofilms in human infections, are presented.

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Section: Biofi Lms From Aerobic Grampositive and Gram-negative Bacteriamentioning

confidence: 99%

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Akers

Cardile

Wenke

et al. 2014

Advances in Experimental Medicine and Biology

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“…There are different ways for evaluating biofilm formation ability such as: microtitre plate, a tube test, radiolabelling, Congo red agar plate test and confocal laser scanning microscopy. The most popular of them is crystal violet microtitre plate assay (17).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Biofilm Formation Among Methicillin Resistance Staphylococcus aureus Isolated From Nasal Carriers

Rezaei¹,

Moniri²,

Mousavi³

et al. 2013

Jundishapur J Microbiol

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Background: Methicillin -resistant staphylococcus aureus (MRSA) is associated with serious infections. Having the ability of biofilmformation decrease their susceptibility to antibiotics. Objectives: The aim of this study was to determine the prevalence of biofilm formation among MRSA isolated from nasal carriers in the Beheshti Teaching Hospital in Kashan, Iran. Materials and Methods: A cross-sectional study was conducted in 810 patients referred to emergency department in Beheshti Hospital in Kashan. Sterilized nasal swabs were used for collecting nasal bacteria. Nasal specimens were further recognized as S. aureus strains by standard biochemical tests, and MRSA isolates were detected by disk diffusion method. PCR assay was used for detecting mecA gene in MRSA isolates. The susceptibility of MRSA isolates to amikacin, clindamycin, gentamicin, ciprofloxacin, SXT, erythromycin, tetracycline were determined by using disk diffusion method according to recommendation of CLSI. Biofilm formation ability of MRSA isolates were examined by crystal violet microtitre plate assay and Congo red agar (CRA). Results: Two hundred and ninety six (36.5%) out of 810 isolates were S. aureus. Twenty six (8.8%) of all S. aureus isolates were recognized as MRSA. All the MRSA isolates have the ability of biofilm formation which 15.4%, 19.2% and 65.4% of them were strong, medium and weak biofilm producer respectively. The resistance rate of strong biofilm producer were; erythromycin (100%), clindamycin (75%), ciprofloxacin (75%), SXT (75%), gentamycin (50%), tetracycline (0%), amikacin (0%). Conclusions: High rate of MRSA nasal carrier and having the ability of biofilm formation which decrease their susceptibility to antibiotics, is an alarming for public health. Statistically significant correlation between susceptibility to tetracycline and MRSA carrier was observed.

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“…QS is encoded by the agr operon where agrD encodes the autoinducer. In a study of 168 strains of MRSA, 23 were from patients with device-related infections, 55 were from patients with non-device-related infections, and 90 were from asymptomatic nasal carriers (Kawamura et al, 2011). The device group had a significantly higher prevalence of agr-2 than the colonization group (78.3 % vs 34.4 %, P50.001) and a higher biofilm index compared to agr-1 and agr-3.…”

Section: Biofilms In Mrsa Infectionsmentioning

confidence: 99%