A method for quantitation of alpha crystallin in individual samples of human aqueous humor by radioimmunoassay is described. The concentration of alpha crystallin in aqueous humor from 48 eyes with cataract and three eyes with clear lenses was at or below 0.006 pgJml. Two samples from eyes with heterochromic cataract contained 0.01 1 and 0.012 yg/ml, respectively. Alpha crystallin is probably not among those lens proteins described earlier in normal human aqueous humor.
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