Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.075 View full text
Bradley J. VanMiddlesworth, John G. Dorsey

Abstract: Peak distortion due to the injection was measured as a function of injection solvent strength, volume, mass, retention factor, and column selectivity. The concept of a method's sensitivity (s) to injection solvent strength was mathematically defined as a vector of theoretical plate counts compared to an ideal vector that does not change with injection solvent strength. Near ideal sensitivity (s>0.90) was measured on all columns with all analytes in low volume injections of 1.25 μL. Increasing the injection vol…

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