Quantification of the immunoglobulin classes IgG and IgA in the young and adult pigeon<i>(Columba livia)</i>

Engberg, Ricarda M.; Kaspers, Bernd; Schranner, I.; Kösters, J; Lösch, U.

doi:10.1080/03079459208418859

Cited by 26 publications

(27 citation statements)

References 18 publications

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“…The protein after the purification with gel filtration and anion-exchange columns was estimated to be ϳ5 mg from 1 ml of pigeon serum. This value is within the range of reported concentration of IgG in adult pigeon serum (5.92 mg/ml) (34).…”

Section: Fig 1 Detection Of ␣-Galactosyl Residue On Pigeon Glycoprosupporting

confidence: 65%

N-Glycan Structures of Pigeon IgG

Suzuki

Khoo

Chen

et al. 2003

Journal of Biological Chemistry

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We had shown previously that all major glycoproteins of pigeon egg white contain Gal␣1-4Gal epitopes (Suzuki, N., Khoo, K. H., Chen, H. C., Johnson, J. R., and Lee, Y. C. (2001) J. Biol. Chem. 276, 23221-23229). We now report that Gal␣1-4Gal-bearing glycoproteins are also present in pigeon serum, lymphocytes, and liver, as probed by Western blot with Griffonia simplicifolia-I lectin (specific for terminal ␣-Gal) and anti-P 1 (specific for Gal␣1-4Gal␤1-4GlcNAc␤1-) monoclonal antibody. One of the major glycoproteins from pigeon plasma was identified as IgG (also known as IgY), which has Gal␣1-4Gal in its heavy chains. High pressure liquid chromatography, mass spectrometric (MS), and MS/MS analyses revealed that N-glycans of pigeon serum IgG included (i) high mannose-type (33.3%), (ii) disialylated biantennary complex-type (19.2%), and (iii) ␣-galactosylated complex-type N-glycans (47.5%). Bi-and tri-antennary oligosaccharides with bisecting GlcNAc and ␣1-6 Fuc on the Asn-linked GlcNAc were abundant among N-glycans possessing terminal Gal␣1-4Gal sequences. Moreover, MS/MS analysis identified Gal␣1-4Gal␤1-4Gal␤1-4GlcNAc branch terminals, which are not found in pigeon egg white glycoproteins. An additional interesting aspect is that about two-thirds of high mannose-type N-glycans from pigeon IgG were monoglucosylated. Comparison of the N-glycan structures with chicken and quail IgG indicated that the presence of high mannose-type oligosaccharides may be a characteristic of these avian IgG. Pigeon (Columba livia) egg white (PEW)1 is a rich source of glycoproteins containing Gal␣1-4Gal (galabiose). All four major PEW glycoproteins, ovotransferrin, two ovalbumins, and ovomucoid, contain Gal␣1-4Gal (1), and all of their constituent N-glycans were found to possess Gal␣1-4Gal at the non-reducing termini of tri-, tetra-, and penta-antennary structures (2). No sialylation is found on the branch that contains the Gal␣1-4Gal sequence. Because biosynthesis and glycosyl modification of major egg white glycoproteins of chicken are carried out in tubular gland cells of oviduct (3), the most likely site of fabricating Gal␣1-4Gal linkages on the PEW glycoproteins would be in the corresponding cells of pigeon oviduct.Gal␣1-4Gal in glycoproteins is, however, uncommon in nature. Mammals (e.g. human, pig, rat, mouse, and hamster) express Gal␣1-4Gal mostly on glycolipids (4 -8), as in P 1 (Gal␣1-4Gal␤1-4GlcNAc␤1-3Gal␤1-4Glc␤1-1Cer) and P k (Gal␣1-4Gal␤1-4Glc␤1-1Cer) antigens, but rarely on glycoproteins. An exception in mammals is the case of glycoproteins with Gal␣1-4Gal in hydatid cyst fluid and membrane infected by tapeworm Echnococcus granulosus (9 -11). In animals other than birds and mammals, the only recorded presence of Gal␣1-4Gal was in O-glycosides such as those found in an insect cell line from Mamestra brassicae (12) or egg jelly mucins from amphibians, such as Xenopus laevis (13), Rana clamitans (14), and Ambystoma mexicanum (15).The presence of Gal␣1-4Gal or substances similar to P 1 antigen on glycoproteins is limited even...

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Section: Fig 1 Detection Of ␣-Galactosyl Residue On Pigeon Glycoprosupporting

confidence: 65%

N-Glycan Structures of Pigeon IgG

Suzuki

Khoo

Chen

et al. 2003

Journal of Biological Chemistry

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“…Antibodies to Chlamydophila psittaci in sera of juveniles acquired by the transovarial route is suggested as the natural method of protection of birds against ornithosis (Kocianova et al 1993). Immunoglobulins in crop milk of the parents are also believed to increase local intestinal immunity (Engberg et al 1992). The development of active immunity mainly depends on antigen contacts that increase with juvenile independence.…”

Section: Discussionmentioning

confidence: 99%

Phenotype-dependent selection of juvenile urban Feral PigeonsColumba livia

Haag‐Wackernagel¹,

Heeb²,

Leiss³

2006

Bird Study

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“…Thus, it does not seem that Cory's shearwater-and maybe procellariiforms in general-rely on oral transfer of antibodies to their offspring (Apanius 1998). However, it should be noted that crop milk antibodies detected in pigeons were mostly IgA (Engberg et al 1992), which we did not measure in our oil samples and are not likely to reach the bloodstream of the chick. Such antibodies could nevertheless provide local protection at the gut level in a way similar to what is suspected in mammals (e.g., Sadeharju et al 2007) and could also have profound effect on the establishment of the gut microbiota of these chicks (Rogier et al 2014).…”

Section: Transgenerational Immunization Of Slow-growing Offspringmentioning

confidence: 99%

“…Although the egg yolk represents the main route of transfer, chicks of some species can receive additional antibodies by being later fed an immunoglobulin-rich substance produced in parental crops (Engberg et al 1992), as is already known in Columbiformes and Phoenicopteriformes (i.e., pigeons and flamingos). In shearwater, both parents provide their newborn with a crop oil that could play a similar role.…”

Section: Transgenerational Immunization Of Slow-growing Offspringmentioning

confidence: 99%

Long Antibody Persistence and Transgenerational Transfer of Immunity in a Long-Lived Vertebrate

Ramos

Garnier

González‐Solís

et al. 2014

The American Naturalist

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. abstract: Although little studied in natural populations, the persistence of immunoglobulins may dramatically affect the dynamics of immunity and the ecology and evolution of host-pathogen interactions involving vertebrate hosts. By means of a multiple-year vaccination design against Newcastle disease virus, we experimentally addressed whether levels of specific antibodies can persist over several years in females of a long-lived procellariiform seabird-Cory's shearwater-and whether maternal antibodies against that antigen could persist over a long period in offspring several years after the mother was exposed. We found that a single vaccination led to high levels of antibodies for several years and that the females transmitted antibodies to their offspring that persisted for several weeks after hatching even 5 years after a single vaccination. The temporal persistence of maternally transferred antibodies in nestlings was highly dependent on the level at hatching. A second vaccination boosted efficiently the level of antibodies in females and thus their transfer to offspring. Overall, these results stress the need to consider the temporal dynamics of immune responses if we are to understand the evolutionary ecology of host-parasite interactions and trade-offs between immunity and other life-history characteristics, in particular in long-lived species. They also have strong implications for conservation when vaccination may be used in natural populations facing disease threats.

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Quantification of the immunoglobulin classes IgG and IgA in the young and adult pigeon(Columba livia)

Cited by 26 publications

References 18 publications

N-Glycan Structures of Pigeon IgG

N-Glycan Structures of Pigeon IgG

Phenotype-dependent selection of juvenile urban Feral PigeonsColumba livia

Long Antibody Persistence and Transgenerational Transfer of Immunity in a Long-Lived Vertebrate

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