Quantification of Glycyrrhizin Biomarker in Glycyrrhiza Glabra Rhizome and Baby Herbal Formulations by Validated Rp-HPTLC Methods

Alam, Prawez; Foudah, Ahmed I.; Zaatout, Hala H.; Kamal, YT; Abdel‐Kader, Maged S.

doi:10.21010/ajtcam.v14i2.21

Cited by 12 publications

(5 citation statements)

References 11 publications

(2 reference statements)

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“…Hence, all these phytochemicals revealed in licorice extract have great therapeutical properties. Previous chemical studies also reported that licorice had phytochemicals includes chalcones, phenols, sterols, saponins, and many others that had strong antioxidant, antibacterial activity, and various others (Alam et al, 2017). Another earlier study showed the presence of phytochemicals steroids, alkaloids, tannins, terpenoids, flavonoids, and others in chloroform extract of Glycyrrhiza glabra (Chandra and Gunasekaran, 2017).…”

Section: Results: Phytochemical Analysis: Biochemical Screeningmentioning

confidence: 93%

Effect of different solvents on phytochemical and antimicrobial potential of licorice extract

Ahmed

Ahmad²,

Izhar

et al. 2022

World J. Biol. Biotechnol.

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Effect of the solvents ethanol, acetone, and distilled water at several concentrations on the antimicrobial potential, antioxidant activity, total flavonoid and phenolic content, and biochemical screening was studied for licorice extract. All extract at 100% solvent concentration showed the presence of phenols, carbohydrates, alkaloids, steroids, coumarins, tannins, saponins, and flavonoids followed by 75% and 50% concentrations except fatty acids, proteins, and quinones. The highest total phenolic content (114.17 mg/GAE) was observed in 100% ethanol extract and total flavonoid content (56 mg/QE) in 100% distilled water extract. The ethanol 100% extract had greatest antioxidant activity (62.3%) at 400µg/mL concentration. Antimicrobial effect was recorded higher against Streptococcus pyogenes (21mm) and Staphylococcus aureus (20mm) in 100% ethanol extract. Gram-negative bacteria showed resistance against all licorice extract. Due to various bioactive compounds in licorice extract it would be a natural antioxidant and antimicrobial agent in the pharmaceutical and food industry with multiple health benefits

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Section: Results: Phytochemical Analysis: Biochemical Screeningmentioning

confidence: 93%

Effect of different solvents on phytochemical and antimicrobial potential of licorice extract

Ahmed

Ahmad²,

Izhar

et al. 2022

World J. Biol. Biotechnol.

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show abstract

“…Although HPLC remains a primary method, various advanced analytical techniques have been proposed. These encompass semipreparative reversed‐phase HPLC with a photodiode array, 13 ultrahigh‐performance liquid chromatography–tandem mass spectrometry, 14 reversed‐phase high‐performance thin‐layer chromatography, 15 HPTLC, 16 and two‐dimensional quantitative nuclear magnetic resonance 17 . A few of these methods are distinct modifications or advancements of HPLC 18–20 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous rapid detection of glycyrrhizin and sennoside A in Daiokanzoto samples by lateral flow immunoassay

Ochi,

Nuntawong,

Chaingam

et al. 2024

Phytochemical Analysis

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IntroductionGlycyrrhizin (GLY) and sennoside A (SA) are characteristic bioactive marker compounds of the Kampo medicine Daiokanzoto. Their accurate detection in blends of Rhei rhizoma and Glycyrrhizae radix of several species (4:1 or 4:2) is essential for quality control and to ensure therapeutic efficacy. A rapid, efficient assay can significantly facilitate their detection.ObjectiveTo establish a rapid qualitative assay for GLY and SA detection, a lateral flow immunoassay (LFA) was developed using specific monoclonal antibody (mAb) nanoparticles.MethodologyThis assay harnesses the competitive binding of mAb nanoparticles to the immobilized analytes on test strips and free analytes in the samples. Two conjugates for detecting GLY and SA, GLY–bovine serum albumin and SA–human serum albumin, were separately immobilized on the test zones of LFA strips. The detection mechanism is reliant on the visual detection of color changes in the test zones.ResultsWhen GLY and SA were present in samples, they contended with the immobilized conjugates on the strip to bind with the mAb nanoparticles and produced distinct color patterns in the test zones. The limits of detection of the assay for GLY and SA were both 3.13 μg/mL. The capability of the LFA was substantiated using plant samples and Daiokanzoto, and its alignment with indirect competitive ELISA results was confirmed.ConclusionThe introduced LFA is a groundbreaking procedure that offers a rapid, straightforward, and sensitive method for simultaneously detecting GLY and SA in Daiokanzoto samples. It is instrumental in ensuring product quality.

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“…In modern food production, licorice is widely used in beverages, beer, meat and other foods as sweeteners, antioxidants, antimicrobials, foaming agents and flavor enhancers. It is a recognized food additive in the Europe, the United States and China (Montoro et al, 2011;Di Lorenzo et al, 2015;Li et al, 2016;Xu et al, 2016;Alam et al, 2017;Indhu and Shajahan, 2018). Modern pharmacological studies have shown that licorice has numerous effects, such as anti-inflammatory, antiviral, antibacterial, and immunomodulatory, as well as certain hypoglycemic, anti-obesity, and detoxification effects (Yang et al, 2015;Luo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Traditional Uses, Pharmacological Effects, and Molecular Mechanisms of Licorice in Potential Therapy of COVID-19

et al. 2021

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The current Coronavirus disease 2019 (COVID-19) pandemic has become a global challenge, and although vaccines have been developed, it is expected that mild to moderate patients will control their symptoms, especially in developing countries. Licorice, not only a food additive, but also a common traditional Chinese herbal medicine, which has several pharmacological effects, such as anti-inflammation, detoxification, antibacterial, antitussive, and immunomodulatory effects, especially in respiratory diseases. Since the outbreak of COVID-19, glycyrrhizin, glycyrrhizin diamine and glycyrrhizin extract have been widely studied and used in COVID-19 clinical trials. Therefore, it is a very interesting topic to explore the material basis, pharmacological characteristics and molecular mechanism of licorice in adjuvant treatment of COVID-19. In this paper, the material basis of licorice for the prevention and treatment of COVID-19 is deeply analyzed, and there are significant differences among different components in different pharmacological mechanisms. Glycyrrhizin and glycyrrhetinic acid inhibit the synthesis of inflammatory factors and inflammatory mediators by blocking the binding of ACE 2 to virus spike protein, and exert antiviral and antibacterial effects. Immune cells are stimulated by multiple targets and pathways to interfere with the pathogenesis of COVID-19. Liquiritin can prevent and cure COVID-19 by simulating type I interferon. It is suggested that licorice can exert its therapeutic advantage through multi-components and multi-targets. To sum up, licorice has the potential to adjuvant prevent and treat COVID-19. It not only plays a significant role in anti-inflammation and anti-ACE-2, but also significantly improves the clinical symptoms of fever, dry cough and shortness of breath, suggesting that licorice is expected to be a candidate drug for adjuvant treatment of patients with early / mild COVID-19.

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Quantification of Glycyrrhizin Biomarker in Glycyrrhiza Glabra Rhizome and Baby Herbal Formulations by Validated Rp-HPTLC Methods

Cited by 12 publications

References 11 publications

Effect of different solvents on phytochemical and antimicrobial potential of licorice extract

Effect of different solvents on phytochemical and antimicrobial potential of licorice extract

Simultaneous rapid detection of glycyrrhizin and sennoside A in Daiokanzoto samples by lateral flow immunoassay

Traditional Uses, Pharmacological Effects, and Molecular Mechanisms of Licorice in Potential Therapy of COVID-19

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