Quantification of flavonoids by UPLC-MS and its antibacterial activity from Brassica oleracea var. Capitata L. Primary tabs

Abstract

Foodborne diseases remain considerable topic of concern and food safety is an important health, social and economical issue. Food borne illnesses caused by the contamination of microbes increases the concerns to find alternate sources which are safe to human health and environment. This study is the first attempt designed to determine the flavonoid content by UPLC-MS in the Indian variety of Brassica oleracea var. capitata L.(organic white cabbage) cultivated in Mysuru region and their potent antibacterial property against food borne pathogens. In the results of the present study, different flavonoids such as genistein > kaempherol > naringenin and catechin was observed which possess antibacterial activity. The antibacterial results showed that the flavonoids and there derivatives have potent antibacterial activity against the gram positive Staphylococcus aureus and the gram negative E. coli. Hence it can be concluded from the study that potential for developing antibacterial from organic white cabbage appears rewarding for the development of phytomedicine to act against microbes.

Flavonoids: isolation, characterization, and health benefits

“…Genistein, kaempferol, naringenin, and catechin isolated from Brassica oleracea var. Capitata L. possessed anti-bacterial activity against E. coli and S. aureus [52].…”

Phytochemical profile and antimicrobial activity of cashew apple (Anacardium occidentale L.) extract

“…The freeze dried cashew apple extract was prepared with slight modification as per the procedure described [7]. Lyophilized sample was macerated with methanol (10 ml/gm of weight) and extracted for 18 hrs before filtration.…”

“…The antibacterial activity of cashew apple extract was determined by disc diffusion method [7] against both Grampositive (Streptococcus pyogenes, Micrococcus luteus, Entrococcus faecalis, Bacillus cereus) and Gram-negative (Salmonella typhimurium) bacteria. Each sterile disc (6 mm) was loaded with 10 µl of cashew apple extract (1 mg disc -1 ) and placed at equidistant on nutrient agar plates seeded with test bacteria (1.5 × 10 8 CFU mL -1 ).…”

“…In addition to this, they serve as alternative sources for western medicines that are expensive, synthetic and as consequence, may have adverse side effects [1]. In our laboratory, various medicinal plants such as Moringa oleifera, Ficus benghalensis, Brassica oleracea and Morus indica (MI) varieties have been screened for their proximate composition, phytochemical profile [2], antioxidant [3], anti-hypercholesterolemic [4], antimutagenic [5], anti-diabetic [6], antimicrobial [7] and protease activities in both in-vitro and ex-vivo models [8].…”

Acacia senegal Extract Rejuvenates the Activity of Phenicols on Selected Enterobacteriaceae Multi Drug Resistant Strains

“…Another traditional healer treats dysentery, gastrointestinal pain, leprosy, nervous sensory and digestive disorders, toothache, rheumatism, stomach ulcers, jaundice, intestinal parasites, and syphilis by roots, leaves, barks, and gum of A. seyal [10,13,14]. In recent studies, extracts of A. senegal and A. seyal have shown good activity against many agents responsible for infectious diseases [12,[15][16][17] and have interesting chemical components such as phenolic compounds, flavonoids, tannins, or terpenes recognized as having a strong antibacterial potential [8,18]. There is no study regarding possible adjuvant propriety of their extracts and their capability to permeabilize the bacterial membrane.…”