Quantification of citrulline by parallel fragmentation monitoring — A novel method using graphitized carbon nanoparticles and MALDI-TOF/TOF mass spectrometry

Ng, Eddy Wing Yin; Lam, Hugh Simon; Ng, Pak Cheung; Poon, Terence Chuen Wai

doi:10.1016/j.cca.2012.10.039

Cited by 10 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Working suspension of the graphitized carbon nanoparticles was freshly prepared by mixing the stock solution with equal volume of fructose solution (50 mg/mL fructose in water), as previously described. 14) Graphene akes were initially washed with ACN and water, and then resuspended in 50% ethanol in water (v/v) at a concentration of 2 mg/mL. Graphitized carbon nanoparticles and graphene ake solution were dispersed by sonication for 10 min before use.…”

Section: Standard Solutions Graphitized Carbon Nanoparticles and Gramentioning

confidence: 99%

“…Unless otherwise stated, the instrument conditions for re ectron and LIFT tandem MS as well as peak detection algorithm of Flex Analysis so ware (version 2.0) were identical to those previously reported. 14) PFM was also performed as previously reported. 14) Brie y, the setting of the timed ion selector gate was optimized to obtain the maximum amount of precursor ions for both citrulline and [ureido-…”

Section: Maldi-tof/tof Mass Spectrometry and Pfmmentioning

confidence: 99%

“…14) PFM was also performed as previously reported. 14) Brie y, the setting of the timed ion selector gate was optimized to obtain the maximum amount of precursor ions for both citrulline and [ureido-…”

Section: Maldi-tof/tof Mass Spectrometry and Pfmmentioning

confidence: 99%

“…Matrix-assisted laser desorption/ionization time-of-ight/time-of-ight mass spectrometry (MALDI-TOF/TOF MS) has potentials to be applied to the quali cation and quanti cation of small molecules. [11][12][13][14] It can avoid the need for LC separation prior to MS analysis, resulting in reduction of turn-around time. Moreover, it can allow quick assessment of result quality by identifying interfering signal peaks near the target analyte in the entire MS and/or MS/MS spectra.…”

Section: Introductionmentioning

confidence: 99%

“…17) For example, among the common amino acids in human specimens, there are 3 pairs of structural analogs with a mass di erence about 1 Da, and they are asparagine (MW 132.12) and aspartic acid (MW 133.10), glutamine (MW 146.15) and glutamic acid (MW 147.13), and arginine (MW 174.20) and citrulline (MW 175.19). Recently, using citrulline for proof-ofconcept, a novel method named parallel fragmentation monitoring (PFM), which is an analogue of SRM, has been developed for small molecules quanti cation by using MALDI-TOF/TOF MS. 14) In contrast to ESI-QqQ-MS in SRM mode, MALDI-TOF/TOF MS provides mass spectra covering the entire acquisition range during PFM analysis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Study of Isobaric Interference in Quantification of Citrulline by Parallel Fragmentation Monitoring

Lam

et al. 2014

Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Parallel Fragmentation Monitoring (PFM), which is an analogue of selected reaction monitoring (SRM), is a recently developed method for quanti cation of small molecules by MALDI-TOF/TOF mass spectrometry (MS). It is well known that isobaric interference substances can be occasionally present in complex biological samples, and a ect the accuracy of measurement by SRM. Unfortunately, by design it is not possible to assess whether isobaric interference happens in a SRM analysis. In contrast, the unique design of PFM should allow quick inspection for isobaric interference and subsequent correction. In this study, using arginine as an example, interference e ect of isobaric structural analogs on the quanti cation of citrulline by PFM was evaluated. Our results showed that the presence of arginine a ected the measured concentrations of citrulline standard solutions in a concentration dependent manner. Such interference could be observed readily in the MS/MS spectra, and contributed by + fragment ion, which was used as the report ion for quanti cation. However, such interference could be mathematically eliminated or minimized through estimation of the signal intensity of the 13 C-isotopic peak of [arginine+H-NH 3 ] + from the intensity of the corresponding monoisotopic peak according to isotope distribution of elements. Furthermore, the presence of interfering fragment ions could be avoided by optimizing MALDI ionization condition. In conclusion, isobaric interference can happen in PFM, but can be easily identi ed in the mass spectra and eliminated (minimized) with simple methods.

show abstract

Section: Standard Solutions Graphitized Carbon Nanoparticles and Gramentioning

confidence: 99%

Section: Maldi-tof/tof Mass Spectrometry and Pfmmentioning

confidence: 99%

Section: Maldi-tof/tof Mass Spectrometry and Pfmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Study of Isobaric Interference in Quantification of Citrulline by Parallel Fragmentation Monitoring

Lam

et al. 2014

Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

show abstract

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Wong

Poon

2013

Chemical Diagnostics

View full text Add to dashboard Cite

The concept of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was first reported in 1985. Since then, MALDI MS technologies have been evolving, and successfully used in genome, proteome, metabolome, and clinical diagnostic research. These technologies are high-throughput and sensitive. Emerging evidence has shown that they are not only useful in qualitative and quantitative analyses of proteins, but also of other types of biomolecules, such as DNA, glycans, and metabolites. Recently, parallel fragmentation monitoring (PFM), which is a method comparable to selected reaction monitoring, has been reported. This highlights the potentials of MALDI-TOF/TOF tandem MS in quantification of metabolites. Here we critically review the applications of the major MALDI MS technologies, including MALDI-TOF MS, MALDI-TOF/TOF MS, SALDI-TOF MS, MALDI-QqQ MS, and SELDI-TOF MS, to the discovery and quantification of disease biomarkers in biological specimens, especially those in plasma/serum specimens. Using SELDI-TOF MS as an example, the presence of systemic bias in biomarker discovery studies employing MALDI-TOF MS and its possible solutions are also discussed in this chapter. The concepts of MALDI, SALDI, SELDI, and PFM are complementary to each other. Theoretically, all these technologies can be combined, leading to the next generation of the MALDI MS technologies. Real applications of MALDI MS technologies in clinical diagnostics should be forthcoming.

show abstract

Nanoparticles for Mass Spectrometry Applications

Larguinho

Capelo

Baptista

2015

Handbook of Nanoparticles

View full text Add to dashboard Cite

Nanotechnology has led to the development of new and improved materials, and particular emphasis has been directed toward nanoparticles and their multiple bio-applications. Nanoparticles exhibit size-, shape-, and composition-dependent properties, e.g., surface plasmon resonance and photothermal properties, which may potentially enhance laser desorption/ionization systems for mass spectrometry-based analysis of biomolecules. Also, nanoparticles possess high surface to volume ratio that can be easily derivatized with a wide range of ligands with different functional groups. Surface modification makes nanoparticles advantageous for sample preparation procedures prior to detection by mass spectrometry. Moreover, it allows the synthesis of affinity probes, which promotes interactions between nanoparticles and analytes, greatly enhancing the ionization efficiency.This chapter provides a comprehensive discussion on the use of nanoparticles for mass spectrometryrelated applications, from sample preparation methodologies to ionization surfaces. Applications will focus on nanoparticle size, composition, and functionalization, as a comparative point of view on optimal characteristics toward maximization of bioassay efficiency.

show abstract

Quantification of citrulline by parallel fragmentation monitoring — A novel method using graphitized carbon nanoparticles and MALDI-TOF/TOF mass spectrometry

Cited by 10 publications

References 28 publications

Study of Isobaric Interference in Quantification of Citrulline by Parallel Fragmentation Monitoring

Study of Isobaric Interference in Quantification of Citrulline by Parallel Fragmentation Monitoring

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Nanoparticles for Mass Spectrometry Applications

Contact Info

Product

Resources

About