Quantification of Andrographolide Isolated from &lt;i&gt;Andrographis paniculata&lt;/i&gt; Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC

Syukri, Yandi; Martien, Ronny; Lukitaningsih, Endang; Nugroho, Agung Endro

doi:10.22146/ijc.21163

Cited by 23 publications

(19 citation statements)

References 19 publications

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“…The average retention time of andrographolide was 2.58 min under the studied condition. This retention time was faster than that reported by a previous study (4.3 min) [3]. Having faster retention time can be beneficial in terms of analysis time and amount of mobile phase required for routine analysis.…”

Section: Fig 3: Calibration Curve Of Andrographolide In Methanol Prementioning

confidence: 45%

“…Bulk material quality affects the quality of the finished product. Several authors have studied the quantification of andrographolide isolated from Andrographis paniculata herbs [3,4,20,21], but none of them reported the determination of andrographolide bulk material's identity and purity. ATR-FTIR spectroscopy offers a rapid and direct analytical technique without sample preparation as the advantage [22,23].…”

Section: Fig 1: the Chemical Structure Of Andrographolidementioning

confidence: 99%

“…Data acquisition and analyses were performed using Empower software. This chromatographic condition was adapted from a previously reported technique [3] with a slight modification in the ratio of mobile phase and also detection wavelength.…”

Section: Chromatographic Conditionmentioning

confidence: 99%

“…This compound becomes the point of interest since A. paniculata herb is widely used as a traditional medicine in several Asian countries such as India, Indonesia, China, Malaysia, and Thailand [1,2]. A large number of studies were conducted to isolate, identify, and quantify this substance [3][4][5] as well as assess its pharmacological activities [6][7][8], and its safety [9,10]. Andrographolide is reported to exhibit various biological activities, including antidiabetic [11], anticancer, anti-inflammatory [6], antivirus, and hepatoprotective activities.…”

Section: Introductionmentioning

confidence: 99%

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Employment of Atr-Ftir and HPLC-Uv Method for Detection and Quantification of Andrographolide

Indrati¹,

Martien

Rohman

et al. 2018

Int J App Pharm

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Objective: The purpose of this present study was to describe the employment of infrared (IR) spectroscopy and high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method in determining the identity and purity of bulk material containing andrographolide.Methods: Attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy was carried out in transmittance mode to investigate the molecular vibration of the bulk material component and the similarity of functional groups between bulk material and standard andrographolide. Meanwhile, the liquid chromatographic analyses were performed on a reversed-phase C18 column and under UV detection at 224 nm to determine the andrographolide content of the bulk material.Results: The obtained ATR-FTIR spectra indicated that functional group of the bulk material were in close similarity with those of standard andrographolide. The linearity of the evaluated method achieved over a concentration range of 1 to 60 μg/ml with a high correlation coefficient (0.999). By using the studied HPLC-UV method, the andrographolide of bulk material was found to be 98.12% (retention time of 2.58 min).Conclusion: The studied HPLC-UV method of andrographolide determination is accurate, precise, selective, and brief in terms of analysis time. The studied method, therefore, provides a rapid and reliable assessment for identifying and determining the purity of andrographolide bulk material.

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Section: Fig 3: Calibration Curve Of Andrographolide In Methanol Prementioning

confidence: 45%

Section: Fig 1: the Chemical Structure Of Andrographolidementioning

confidence: 99%

Section: Chromatographic Conditionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Employment of Atr-Ftir and HPLC-Uv Method for Detection and Quantification of Andrographolide

Indrati¹,

Martien

Rohman

et al. 2018

Int J App Pharm

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“…Hence, it shows promise for applications in process simulation in pharmaceutical and nutraceutical industries. Previous researchers have also conducted quantitative analyses of the process of extracting bioactive compounds from natural materials [50][51][52]. The value of the Fourier number (Fo) based on the thickness of the pericarp was found to be ~0.185, which is relatively large.…”

Section: Validation Of the Model Using Experimental Resultsmentioning

confidence: 99%

A Mechanistic Model of Mass Transfer in the Extraction of Bioactive Compounds from Intact Sorghum Pericarp

et al. 2019

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The extraction of phytochemical compounds from intact red sorghum grains was developed as an alternative process for producing bioactive material in the pharmaceutical industry. A mechanistic model is needed to better understand the process and enable predictive simulations for designing commercial-scale extraction systems. This paper presents a mathematical model for predicting phytochemical concentrations in the solvent and inside the pericarp of the grain at different positions during the extraction. The model is based on the mass transfer mechanism from inside the pericarp to its solid surface by diffusion, and then from the surface to a solvent during the extraction of bioactive compounds. It was numerically solved while using finite-difference approximation. The parameters considered were effective diffusivity inside the pericarp (Dep), mass transfer coefficient from the pericarp surface to the solvent (kc), and distribution coefficient (H). The model simulates the extraction performance, including the yield and bioactive compounds’ concentrations in the extract and inside the pericarp at various positions and times. A sensitivity analysis of the changes in each involved parameter provided sufficient information for increasing the performance of the model. A validation test that compared the results of the simulation with those of established analytical solutions showed that the model has high accuracy. Hence, the model can be applied in quantitative evaluations to improve productivity in the pharmaceutical industry.

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Nanophytosomes Loading Andrographis paniculata Hydroalcoholic Extract: Promising Drug Delivery for Hepatoprotective Efficacy

Karekar

Killedar²,

More³

2023

J Pharm Innov

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Quantification of Andrographolide Isolated from <i>Andrographis paniculata</i> Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC

Abstract: This research was aimed to quantification of andrographolide isolated from A. paniculata Ness found in a traditional market in

Cited by 23 publications

References 19 publications

Employment of Atr-Ftir and HPLC-Uv Method for Detection and Quantification of Andrographolide

Employment of Atr-Ftir and HPLC-Uv Method for Detection and Quantification of Andrographolide

A Mechanistic Model of Mass Transfer in the Extraction of Bioactive Compounds from Intact Sorghum Pericarp

Nanophytosomes Loading Andrographis paniculata Hydroalcoholic Extract: Promising Drug Delivery for Hepatoprotective Efficacy

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