Quantification of adipokinetic hormones I and II in the corpora cardiaca of Schistocerca gregaria and Locusta migratoria

Isolation, physiological characterization, release and sequence elucidation of a hypertrehalosaemic neuropeptide from the corpus cardiacum of the stick insect, Sipyloidea sipylus

“…The new methodology worked well with corpora cardiaca of Lmigratoria, from which AKH I and AKH 11-L are released, with octopamine serving as a putative neurotransmitter (Pannabecker & Orchard, 1986). In our system AKH I and AKH 11-L are released in levels apparently proportional to their abundance in the corpus cardiacum; the ratio of compound I to compound I1 was about 6:1, thus, similar to the ratio found in a corpus cardiacum extract (Siegert & Mordue, 1986b).…”

“…The hyperlipaemic activity in S.sipylus shows marked quantitative changes in its content during development; activity is detectable in low amounts on day 1 of the second stadium. Recently, the amounts of the adipokinetic hormones I and I1 have been quantified in the corpora cardiaca of migratory and desert locusts (Siegert & Mordue, 1986b); in Xgregaria the hormone content increases about 5-fold from 190 pmol (AKH I) and 50 pmol (AKH 11-S) in early fifth instar larvae to about 900-1200 pmol and 180-260 pmol in adults more than 6 weeks old. The corpora cardiaca from the migratory locust contained considerably smaller amounts of AKH I and AKH 11-L, but the increases from early fifth instar to late adult life were also about…”

Isolation, physiological characterization, release and sequence elucidation of a hypertrehalosaemic neuropeptide from the corpus cardiacum of the stick insect, Sipyloidea sipylus

“…The new methodology worked well with corpora cardiaca of Lmigratoria, from which AKH I and AKH 11-L are released, with octopamine serving as a putative neurotransmitter (Pannabecker & Orchard, 1986). In our system AKH I and AKH 11-L are released in levels apparently proportional to their abundance in the corpus cardiacum; the ratio of compound I to compound I1 was about 6:1, thus, similar to the ratio found in a corpus cardiacum extract (Siegert & Mordue, 1986b).…”

“…The hyperlipaemic activity in S.sipylus shows marked quantitative changes in its content during development; activity is detectable in low amounts on day 1 of the second stadium. Recently, the amounts of the adipokinetic hormones I and I1 have been quantified in the corpora cardiaca of migratory and desert locusts (Siegert & Mordue, 1986b); in Xgregaria the hormone content increases about 5-fold from 190 pmol (AKH I) and 50 pmol (AKH 11-S) in early fifth instar larvae to about 900-1200 pmol and 180-260 pmol in adults more than 6 weeks old. The corpora cardiaca from the migratory locust contained considerably smaller amounts of AKH I and AKH 11-L, but the increases from early fifth instar to late adult life were also about…”

Identification and purification of two precursors of the insect neuropeptide adipokinetic hormone

“…Each produces a single peak, which comigrates with synthetic standards of either AKH I or II. That the AKH peaks identified by size-exclusion chromatography are pure and authentic has also been independently shown quantitatively by Siegert and Mordue (1986).…”

Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca

“…Considering possible losses this indicates 0.7-2 pmol per pair. This shows that in the corpora cardiaca of M. sextu there is much less AKH than in locusts, where estimates run as high as 1,200 pmol [15]. Since locusts fly longer, it could reflect a difference in the physiology of the insects.…”

Isolation, physiological characterization, release and sequence elucidation of a hypertrehalosaemic neuropeptide from the corpus cardiacum of the stick insect, Sipyloidea sipylus

“…The new methodology worked well with corpora cardiaca of Lmigratoria, from which AKH I and AKH 11-L are released, with octopamine serving as a putative neurotransmitter (Pannabecker & Orchard, 1986). In our system AKH I and AKH 11-L are released in levels apparently proportional to their abundance in the corpus cardiacum; the ratio of compound I to compound I1 was about 6:1, thus, similar to the ratio found in a corpus cardiacum extract (Siegert & Mordue, 1986b).…”

“…The hyperlipaemic activity in S.sipylus shows marked quantitative changes in its content during development; activity is detectable in low amounts on day 1 of the second stadium. Recently, the amounts of the adipokinetic hormones I and I1 have been quantified in the corpora cardiaca of migratory and desert locusts (Siegert & Mordue, 1986b); in Xgregaria the hormone content increases about 5-fold from 190 pmol (AKH I) and 50 pmol (AKH 11-S) in early fifth instar larvae to about 900-1200 pmol and 180-260 pmol in adults more than 6 weeks old. The corpora cardiaca from the migratory locust contained considerably smaller amounts of AKH I and AKH 11-L, but the increases from early fifth instar to late adult life were also about…”

Identification and purification of two precursors of the insect neuropeptide adipokinetic hormone

“…Each produces a single peak, which comigrates with synthetic standards of either AKH I or II. That the AKH peaks identified by size-exclusion chromatography are pure and authentic has also been independently shown quantitatively by Siegert and Mordue (1986).…”

Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca

“…Considering possible losses this indicates 0.7-2 pmol per pair. This shows that in the corpora cardiaca of M. sextu there is much less AKH than in locusts, where estimates run as high as 1,200 pmol [15]. Since locusts fly longer, it could reflect a difference in the physiology of the insects.…”

Isolation, physiological characterization, release and sequence elucidation of a hypertrehalosaemic neuropeptide from the corpus cardiacum of the stick insect, Sipyloidea sipylus

“…The new methodology worked well with corpora cardiaca of Lmigratoria, from which AKH I and AKH 11-L are released, with octopamine serving as a putative neurotransmitter (Pannabecker & Orchard, 1986). In our system AKH I and AKH 11-L are released in levels apparently proportional to their abundance in the corpus cardiacum; the ratio of compound I to compound I1 was about 6:1, thus, similar to the ratio found in a corpus cardiacum extract (Siegert & Mordue, 1986b).…”

“…The hyperlipaemic activity in S.sipylus shows marked quantitative changes in its content during development; activity is detectable in low amounts on day 1 of the second stadium. Recently, the amounts of the adipokinetic hormones I and I1 have been quantified in the corpora cardiaca of migratory and desert locusts (Siegert & Mordue, 1986b); in Xgregaria the hormone content increases about 5-fold from 190 pmol (AKH I) and 50 pmol (AKH 11-S) in early fifth instar larvae to about 900-1200 pmol and 180-260 pmol in adults more than 6 weeks old. The corpora cardiaca from the migratory locust contained considerably smaller amounts of AKH I and AKH 11-L, but the increases from early fifth instar to late adult life were also about…”

Identification and purification of two precursors of the insect neuropeptide adipokinetic hormone

“…Each produces a single peak, which comigrates with synthetic standards of either AKH I or II. That the AKH peaks identified by size-exclusion chromatography are pure and authentic has also been independently shown quantitatively by Siegert and Mordue (1986).…”

Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca

“…Considering possible losses this indicates 0.7-2 pmol per pair. This shows that in the corpora cardiaca of M. sextu there is much less AKH than in locusts, where estimates run as high as 1,200 pmol [15]. Since locusts fly longer, it could reflect a difference in the physiology of the insects.…”