Quantification analysis of protein and mycelium contents upon inhibition of melanin for Aspergillus niger: a study of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)

Kumaran, Sekar; Abdelhamid, Hani Nasser; Wu, Hui‐Fen

doi:10.1039/c7ra03741d

Cited by 17 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They can be used for the analysis of thermal labile nonvolatile analytes with very low fragmentation. Matrix-assisted laser desorption/ionization–mass spectrometry was applied for the analysis of pathogenic microorganisms and their lysates [37,38,39,40,41,42,43,44,45,46], endotoxins [47], proteins [48,49,50,51], phosphopeptides [52], biothiols [53], surfactants [54], lipids [55], metals ions [56,57,58], metallodrugs [59], surface chemistry of nanoparticles functionalized with synthetic ligands [60], intermediates for quantum dots formation [61], and small molecules [62]. The ionization of an analyte may be assisted using organic matrices [63], ILs [64,65,66], or nanoparticles [67].…”

Section: Introductionmentioning

confidence: 99%

Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Abdelhamid

2018

MPs

Self Cite

View full text Add to dashboard Cite

Ionic liquids (ILs) have advanced a variety of applications, including matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS). ILs can be used as matrices and solvents for analyte extraction and separation prior to analysis using laser desorption/ionization–mass spectrometry (LDI–MS). Most ILs show high stability with negligible sublimation under vacuum, provide high ionization efficiency, can be used for qualitative and quantitative analyses with and without internal standards, show high reproducibility, form homogenous spots during sampling, and offer high solvation efficiency for a wide range of analytes. Ionic liquids can be used as solvents and pseudo-stationary phases for extraction and separation of a wide range of analytes, including proteins, peptides, lipids, carbohydrates, pathogenic bacteria, and small molecules. This review article summarizes the recent advances of ILs applications using MALDI–MS. The applications of ILs as matrices, solvents, and pseudo-stationary phases, are also reviewed.

show abstract

Section: Introductionmentioning

confidence: 99%

Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Abdelhamid

2018

MPs

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is because the membrane proteins play a role in the electron transport chain; therefore NPs did not influence the amino acid yield. 64 , 65 The total amino acid content amounted to 5.8 mg mL −1 for A. niger cell dry weight, 6 mg mL −1 for A. niger –CuS–Che NPs, and 6.2 mg mL −1 for A. niger –CuS–Bio NPs, A. niger –CuS–Che NBs and A. niger –CuS–Bio NBs, respectively (Fig. 8a ).…”

Section: Resultsmentioning

confidence: 99%

Enhanced production of amylase, pyruvate and phenolic compounds from glucose by light‐driven Aspergillusniger–CuS nanobiohybrids

Uddandarao

Lens

2022

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND:The demand for value-added compounds such as amylase, pyruvate and phenolic compounds produced by biological methods has prompted the rapid development of advanced technologies for their enhanced production. Nanobiohybrids (NBs) make use of both the microbial properties of whole-cell microorganisms and the light-harvesting efficiency of semiconductors. Photosynthetic NBs were constructed that link the biosynthetic pathways of Aspergillus niger with CuS nanoparticles. RESULTS: In this work, NB formation was confirmed by negative values of the interaction energy, i.e., 2.31 × 10 8 to −5.52 × 10 8 kJ mol −1 for CuS-Che NBs, whereas for CuS-Bio NBs the values were −2.31 × 10 8 to −4.62 × 10 8 kJ mol −1 for CuS-Bio NBs with spherical nanoparticle interaction. For CuS-Bio NBs with nanorod interaction, it ranged from −2.3 × 10 7 to −3.47 × 10 7 kJ mol −1 . Further, the morphological changes observed by scanning electron microscopy showed the presence of the elements Cu and S in the energy-dispersive X-ray spectra and the presence of CuS bonds in Fourier transform infrared spectroscopy indicate NB formation. In addition, the quenching effect in photoluminescence studies confirmed NB formation. Production yields of amylase, phenolic compounds and pyruvate amounted to 11.2 ∼mol L −1 , 52.5 ∼mol L −1 and 28 nmol ∼L −1 , respectively, in A. niger-CuS Bio NBs on the third day of incubation in the bioreactor. Moreover, A niger cells-CuS Bio NBs had amino acids and lipid yields of 6.2 mg mL −1 and 26.5 mg L −1 , respectively. Furthermore, probable mechanisms for the enhanced production of amylase, pyruvate and phenolic compounds are proposed.CONCLUSION: Aspergillus niger-CuS NBs were used for the production of the amylase enzyme and value-added compounds such as pyruvate and phenolic compounds. Aspergillus niger-CuS Bio NBs showed a greater efficiency compared to A. niger-CuS Che NBs as the biologically produced CuS nanoparticles had a higher compatibility with A. niger cells.

show abstract

“…No significant change was observed by conventional cocultivation of A. niger , while on DSPD, the color of the communicating area of A. niger was darker than other areas in the colony. Research shows that the black color (melanin) of A. niger originated from spores ( Kumaran et al, 2017 ). Monascus spp.…”

Section: Discussionmentioning

confidence: 99%

Cocultivation Study of Monascus spp. and Aspergillus niger Inspired From Black-Skin-Red-Koji by a Double-Sided Petri Dish

Yuan

Chen

2021

Front. Microbiol.

View full text Add to dashboard Cite

Cocultivation is an emerging and potential way to investigate microbial interaction in the laboratory. Extensive researches has been carried out over the years, but some microorganism cocultivation are not easy to implement in the laboratory, especially the fungus-fungus (FF) cocultivation, owing to the obstacles such as fungal different growth rate, limited growing space, hyphae intertwining, and difficulty of sample separation, etc. In this research, a double-sided petri dish (DSPD) was designed and carried out as a tool to study FF cocultivation in the laboratory. A natural FF cocultivation of Monascus spp. and Aspergillus niger inspired from black-skin-red-koji (BSRK), were studied. By using DSPD, the aforementioned obstacles in the FF cocultivation study were overcome through co-culturing Monascus spp. and A. niger on each side of DSPD. The characteristics of monocultured and co-cultured Monascus spp. and A. niger were compared and analyzed, including colonial and microscopic morphologies, and main secondary metabolites (SMs) of Monascus spp. analyzed by high performance liquid chromatography. And a novel SM was found to be produced by Monascus ruber M7 when co-cultured with A. niger CBS 513.88. Since the above mentioned obstacles, were overcome, we obtained good quality of transcriptome data for further analysis. These results indicate that DSPD might be an efficient tool for investigation of microbial interaction, in particular, for FF interaction.

show abstract

Quantification analysis of protein and mycelium contents upon inhibition of melanin for Aspergillus niger: a study of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)

Cited by 17 publications

References 52 publications

Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

Enhanced production of amylase, pyruvate and phenolic compounds from glucose by light‐driven Aspergillusniger–CuS nanobiohybrids

Cocultivation Study of Monascus spp. and Aspergillus niger Inspired From Black-Skin-Red-Koji by a Double-Sided Petri Dish

Contact Info

Product

Resources

About