Qualitative and Quantitative Analysis of Five Bioactive Flavonoids in &amp;lt;i&amp;gt;Salix bordensis&amp;lt;/i&amp;gt; Turcz. by HPLC-DAD and HPLC-ESI-MS

Zhao, Lei; Liu, Lina; Li, Jintian

doi:10.4236/ajac.2014.513094

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Kaempferol 32) and its 7,4ˋ-dimethyl derivative 33) were found to be most prominent constituents in S. bordensis Turcz. (Zhao et al, 2014). Also, kaempferol-7-O-glucoside 34) is a major compound in S. babylonica L. leaves and roots (Khatoon et al, 1988;Singh et al, 2017).…”

Section: Flavonoidsmentioning

confidence: 99%

Phytochemistry, Pharmacology and Medicinal Uses of Plants of the Genus Salix: An Updated Review

et al. 2021

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The Willows (genus Salix), with more than 330–500 species and 200 hybrids, are trees, shrubs or prostrate plants that are widely distributed in Africa, North America, Europe, and Asia. The genus is traditionally used in folk medicine and represents a valuable source of biologically active compounds among them salicin, a prodrug for salicylic acid. Altogether, 322 secondary metabolites were characterized in the genus including flavonoids 94) (flavonols, flavones, flavanones, isoflavones, flavan-3-ols (catechins and procyanidins), chalcones, dihydrochalcone, anthocyanins, dihydroflavonols), phenolic glycosides (76), organic acids (28), and non-phenolic glycosides (17), sterols and terpenes (17), simple phenolics 13) and lignans 7) in addition to volatiles and fatty acids (69). Furthermore, willows exert analgesic, anti-inflammatory, antioxidant, anticancer, cytotoxic, antidiabetic, antimicrobial, antiobesity, neuroprotective and hepatoprotective activities. The current review provides an updated summary of the importance of willows, their chemical composition and pharmacological activities.

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Section: Flavonoidsmentioning

confidence: 99%

Phytochemistry, Pharmacology and Medicinal Uses of Plants of the Genus Salix: An Updated Review

et al. 2021

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“…21,25,26 The major obstacle in phytochemical analysis is a choice of proper extraction methods and highly efficient solvents due to the lack of standardisation of methods for extraction of phenolic compounds from plants. [27][28][29][30][31][32][33] Since the applied extraction conditions vary widely in the literature, the experimental plan was used in this work to determine the optimal conditions for effective extraction of flavonoids from the leaf of I. sibirica L.…”

Section: Introductionmentioning

confidence: 99%

Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

Karpitskiy

Бессонова

Карцова

et al. 2022

Phytochemical Analysis

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Introduction Iris L. are promising in medicine due to the biological activity of extracts. Iris sibirica L. is spread in Russia but its phytochemical composition has not been studied in detail though it is included in the Red Book. For this reason, I. sibirica L. biotechnology is in high demand. One of the key points in biotechnology is the regulation of plant metabolism using phytohormones. Obtaining of chromatographic metabolite profiles allows to control this process. Objective The aim of this study was to develop an approach for effective control of biotechnological raw materials of I. sibirica L. by flavonoid profiles using high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and to investigate the influence of phytohormones in nutrient media on content of flavonoids. Methodology Iris sibirica L. regenerated plants were grown on Murashige–Skoog media with 6‐benzylaminopurine (6‐BAP) and α‐naphtylacetic acid (NAA) additives. To optimise extraction conditions, the design of the experiment was used. Profiles of polyphenols were obtained by HPLC–MS/MS in the positive and negative ionisation modes. Results The process for efficient extraction from leaves of I. sibirica L. were developed. The factors influencing the extraction efficiency of flavonoids have been determined. A total of 36 compounds were identified by HPLC–MS/MS. Among them isoflavones and their glycosides are the main classes. Addition of an auxin‐like hormone increased the non‐polar flavonoid levels, but decreased the polar ones. The variation in concentration of cytokinin (6‐BAP) affected almost all of the analytes. Conclusion The methodology for effective control of I. sibirica L. raw plant material biotechnology was developed by analysing obtained chromatographic polyphenol profiles.

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“…This stationary phase is widely used, providing high separation efficiency and excellent mechanical stability. In addition, silica stationary phases modified by several types of bound phases using C4-C30 alkyl groups, such as octadecyl or C18, have been widely used in HPLC [14]. The stationary phase is used to produce more efficient separation results in combination with good selectivity.…”

Section: Introductionmentioning

confidence: 99%

An HPLC-DAD Method to Quantify Flavonoids in Sonchus arvensis and Able to Classify the Plant Parts and Their Geographical Area through Principal Component Analysis

et al. 2021

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A simple and efficient method has been developed for the simultaneous determination of eight flavonoids (orientin, hyperoside, rutin, myricetin, luteolin, quercetin, kaempferol, and apigenin) in Sonchus arvensis by high-performance liquid chromatography diode array detector (HPLC-DAD). This method was utilized to differentiate S. arvensis samples based on the plant parts (leaves, stems, and roots) and the plant’s geographical origin. The chromatographic separation was carried out on a reverse-phase C18 column by eluting at a flow rate of 1 mL/min using a gradient with methanol and 0.2% aqueous formic acid. In the optimum conditions, the developed method’s system suitability has met the criteria of good separation. The calibration curve shows a linear relationship between the peak area and analyte concentration with a correlation coefficient (r2) > 0.9990. The ranges for the analytes’ limits of detection and quantitation were 0.006–0.015 and 0.020–0.052 µg/mL, respectively. Intra-day and inter-day precision expressed in terms of RSD values were <2%, and the accuracy range based on recovery was 97–105%. The stability of all analytes within 48 h was about 2%. By combining HPLC-DAD fingerprint analysis with chemometrics, the developed method can classify S. arvensis samples based on the plant parts and geographical origin.

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Qualitative and Quantitative Analysis of Five Bioactive Flavonoids in <i>Salix bordensis</i> Turcz. by HPLC-DAD and HPLC-ESI-MS

Cited by 6 publications

References 13 publications

Phytochemistry, Pharmacology and Medicinal Uses of Plants of the Genus Salix: An Updated Review

Phytochemistry, Pharmacology and Medicinal Uses of Plants of the Genus Salix: An Updated Review

Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

An HPLC-DAD Method to Quantify Flavonoids in Sonchus arvensis and Able to Classify the Plant Parts and Their Geographical Area through Principal Component Analysis

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