Qualitative and quantitative analysis of sesquiterpene lactones in Centipeda minima by UPLC–Orbitrap–MS &amp; UPLC-QQQ-MS

Chan, Chi-On; Xie, Xiujuan; Wan, Siu-wai; Gao-li, Zhou; Yuen, Ailsa Chui-ying; Mok, Daniel K. W.; Chen, Sibao

doi:10.1016/j.jpba.2019.05.067

Cited by 25 publications

(13 citation statements)

References 13 publications

(14 reference statements)

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“…Even though ion suppression may affect its sensitivity, the UHPLC‐MS/MS technique does not require the complete separation of the chromatographic peaks of multiple components, provided that the target analysis does not interfere with each other. Therefore, it is the primary choice for the quantitative analysis of complex samples [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Zhao

Wang

et al. 2021

J of Separation Science

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Ribes meyeri leaves are used as traditional Kazakh medicine in China. However, no study on the characterization of the phenolic compounds in R. meyeri leaves has been reported, resulting in the lack of quality control measures and poor standardization. This study was conducted to identify the phenolic compounds in R. meyeri leaves and evaluate their antioxidant and antidiabetic activities. A total of 77 phenolics were tentatively identified by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. Ultra‐high performance liquid chromatography coupled with triple quadrupole mass spectrometry was applied to simultaneously quantify 12 phenolics in R. meyeri leaves. Rutin, epigallocatechin, isoquercitrin, epicatechin, protocatechuic acid, and kaempferol‐3‐O‐rutinoside were abundant in the R. meyeri leaves. The methanol extract and four different extracts enhanced the glucose uptake in 3T3‐L1 adipocytes. The ethyl acetate extracts showed a total phenolic content of 966.89 ± 3.59 mg gallic acid equivalents/g, a total flavonoid content of 263.58 ± 17.09 mg catechin equivalents/g, and good protein‐tyrosine phosphatase‐1B inhibitory activities (IC50: 0.60 ± 0.03 μg/mL). To our knowledge, this work is the first to identify and quantify the major phenolics in R. meyeri leaves.

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Section: Introductionmentioning

confidence: 99%

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Zhao

Wang

et al. 2021

J of Separation Science

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Section: Patient Age Gender Duration Aa (Years) Clinical Type Previous Treatment For Aa Treatment Duration (Months) Comorbidities Salt Bementioning

confidence: 99%

“…Chemical analysis has demonstrated that CM contains different components, including flavones and their glycosides, phenolic and polyphenolic acids, but the main constituent is a sesquiterpene lactone named brevilin A which displayed antiproliferative, anti-bacterial and antiprotozoal effects. 8,[10][11][12] The evidence that brevilin A inhibits STAT1 and STAT3 signaling as well as the expression of their target genes and its ability in attenuation of JAKs activity by blocking the JAKs tyrosine kinase domain JH1 aim our study.…”

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confidence: 97%

Alopecia areata treated with topical and systemic brevilin A: A case series

Mellai

Magri

Leoncini

et al. 2021

Dermatologic Therapy

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Alopecia areata (AA) is an autoimmune T CD8 cell mediated condition clinically characterized by hair loss from single or few small patches to complete hair loss. The management of AA is challenging and all available therapies does not ensure a longterm remission. To assess the safety and efficacy of both systemic and topical brevilin A, a natural compound, in AA patients not responding to other treatments. After obtaining informed consent, we administered off-label brevilin A to 13 adult patients affected by AA, for a period ranging from 6 to 18 months. Medical records for each patient and the severity of alopecia tool (SALT) score before and after brevilin A administration were recorded. The mean SALT score of our patients was 81.03 (SD 34.9) at baseline and 75.8 (SD 37.4) after brevilin A therapy, meaning no statistically significant improvement was observed (P = .2385 Paired t test). However, three multifocal AA (MAA) patients out of four attained an improvement (75%) suggesting that brevilin A may be represent an alternative therapy in this form of AA. Authors conclude that brevilin A could represent in the future a possible effective treatment in MAA forms but further studies are required. K E Y W O R D S alopecia areata, brevilin a Alopecia areata (AA) is an autoimmune nonscarring hair loss mediated by CD8 T cells, with chronic-relapsing course. Clinical aspects range from single patches to whole scalp hair loss (alopecia totalis [AT]) and body hair loss (alopecia universalis [AU]). 1 Disease severity is mutually related with psychological consequences, including anxiety and depression, which commonly afflict AA patients, especially in case of young females. Unfortunately, available therapies have been unsuccessful in influencing the long-term course of AA; thus, treatments of AA represent a challenge and a drug with specific indication is still missing. 2 Autoreactive CD8 + NKG2D+ T cells are the main actors in the pathogenesis of AA, responsible for hair loss, thus all common treatments share a regulatory effect upon the immune system. Among them, two JAK inhibitors have been tested, including tofacitinib (JAK 1/3 inhibitor) and ruxolitinib (JAK 1/2 inhibitor) in oral and topical formulations, with promising results. 3-5 These data could be explained by the evidence that JAK-STAT dependent cytokines, IFN-γ and IL-15, activate autoreactive CD8 + NKG2D+ T cells, and also by JAK inhibitors ability in inducing anagen phase. 6,7 However, these drugs could show adverse effects, especially in case of longterm use. Interestingly, a natural molecule named brevilin A shows a strong effect in JAK-STAT signal inihibition, both in vitro and in vivo studies. 8 Brevilin A is a sesquiterpene lactone isolated from Centipeda minima (CM), a medicinal plant of Asteraceae family distributed in China,

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“…Therefore, in this study, an effective and sensitive UHPLC-QQQ-MS/MS method is established, and a qualitative analysis of six dianthrones is presented. The excellent selectivity and sensitivity achieved for these target compounds in multi reaction monitoring (MRM) mode allowed satisfactory con rmation and quantitation [22]. In addition, the proposed UHPLC-QQQ-MS/MS method is successfully used for dianthrones determination in PMR and PMRP.…”

Section: Introductionmentioning

confidence: 99%

UHPLC-QQQ-MS/MS assay for quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: Application to the standardization of TCMs with endogenous toxicity

Yang¹,

Song²,

Liu³

et al. 2021

Preprint

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Background: The raw and processed roots of Polygonum multiflorum Thunb (PM) are commonly used in clinical practice to treat diverse diseases; however, the reports of hepatotoxicity induced by Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) have emerged worldwide. Thus, it is necessary for researcher to explore the methods to improve its quality standards and further ensure its quality and treatment effect.Methods: In the present study, an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ- MS/MS) method has been optimized and validated for the determination of dianthrones in PMR and PMRP, using bianthronyl as the internal standard. Chromatographic separation with a gradient mobile phase (A: acetonitrile and B: water containing 0.1% formic acid (v/v)) at a flow rate of 0.25 mL/min was achieved on a Waters Acquilty UPLC BEH b) C18 column (2.1 mm × 50 mm, 1.7 µm). A triple quadrupole mass spectrometer (TQMS) was operated in negative ionization mode with multiple reaction monitoring for the quantitative analysis of six dianthrones. Meanwhile, compounds 5 and 6 were further evaluated for cytotoxicity of HepaRG cells by CCK8 assay.Results: The UHPLC-QQQ-MS/MS method was first developed to simultaneous determination of six dianthrones in PMR and PMRP, namely polygonumnolides C1–C4 (1–4), trans-emodin dianthrones (5), and cis-emodin dianthrones (6). The contents of 1~6 in 90 batches of PMR were in the range of 0.027-19.04, 0.022-13.86, 0.073 -15.53, 0.034 -23.35, 0.38-83.67 and 0.29 -67.00 µg/g, respectively. The contents of 1~6 in 86 batches of commercial PMRP were in the range of 0.020-13.03, 0.051-8.94, 0.022-7.23, 0.030 -12.75, 0.098-28.54 and 0.14-27.79 µg/g, respectively. The six dianthrones were almost completely gone after reasonable processing for 24 h. Meanwhile, compounds 5 and 6 showed the inhibitory activity against HepaRG cells with the IC50 values of 10.98 and 15.45 μM, respectively. Furthermore, a systematic five-step strategy to realize the standardization of TCMs with endogenous toxicity is proposed for the first time, involving the establishment of determination methods, determination of the toxic markers, the standardization of processing method, the development of limit standards and benefit-risk assessment.Conclusion: The results of cytotoxicity evaluation of dianthrone indicated that trans-emodin dianthrones (5) and cis-emodin dianthrones (6) could be selected as the toxic markers of PMRP. Taking PMR and PMRP for example, we hope this study provided insight into the standardization and internationalization of endogenous toxic TCMs, with the main purpose of improving public health by scientifically using TCMs to treat diverse complex diseases in future.

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Qualitative and quantitative analysis of sesquiterpene lactones in Centipeda minima by UPLC–Orbitrap–MS & UPLC-QQQ-MS

Cited by 25 publications

References 13 publications

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Alopecia areata treated with topical and systemic brevilin A: A case series

UHPLC-QQQ-MS/MS assay for quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: Application to the standardization of TCMs with endogenous toxicity

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Qualitative and quantitative analysis of sesquiterpene lactones in Centipeda minima by UPLC–Orbitrap–MS & UPLC-QQQ-MS

Cited by 25 publications

References 13 publications

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Alopecia areata treated with topical and systemic brevilin A: A case series

UHPLC-QQQ-MS/MS assay for quantification of dianthrones as&nbsp;potential toxic markers of Polygonum multiflorum Thunb: Application to the standardization of TCMs with endogenous toxicity

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UHPLC-QQQ-MS/MS assay for quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: Application to the standardization of TCMs with endogenous toxicity