Qualitative and Quantitative Analysis

Snyder, Lloyd R.; Kirkland, J. J.; Dolan, John W.

doi:10.1002/9780470508183.ch11

Cited by 10 publications

(1 citation statement)

References 34 publications

(51 reference statements)

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“…We hypothesize that the matrix effect can influence the detection of plant allelopathic compounds: metabolites might interact and impair gramine’s detection during analysis. Considering that both accuracy and precision are subjected to further variables such as the day of analysis, the operator and the instruments, we conclude that our method reaches excellent accuracy and precision in the separation and quantification of gramine levels in seeds and vegetative tissues [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Leite Dias,

Garibay-Hernández,

Brendel

et al. 2023

Plants

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Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%, <7% RSD. The method was successfully applied in a study of the natural range of abundance of gramine, hordenine and their related metabolites, AMI, tryptophan and tyramine, in 22 barley accessions and 10 lupin species. This method provides accurate and highly sensitive chromatographic separation and detection of tryptophan- and tyrosine-derived allelochemicals and is an accessible alternative to LC-MS techniques for routine screening.

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Section: Discussionmentioning

confidence: 99%

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Leite Dias,

Garibay-Hernández,

Brendel

et al. 2023

Plants

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Age-related differences in virtual histology-intravascular ultrasound findings in patients with coronary artery disease

Hong

Jeong

Choi

et al. 2010

Journal of Cardiology

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Formic Acid and Methanol Concentrations in Death Investigations

Wallage

Watterson

2008

Journal of Analytical Toxicology

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Methanol ingestion results in the formation of formic acid, a toxic metabolite that can cause metabolic acidosis. Methanol toxicity is therefore dependent on the amount of methanol ingested, the nature of treatment received, elapsed time since ingestion, and the accumulation of formic acid. Both methanol and formic acid concentrations are determined at this laboratory using headspace gas chromatography. An examination of 12 fatalities attributed to methanol poisoning is presented. Six individuals were found deceased, and their postmortem methanol and formic acid concentrations ranged from 84 to 543 mg/dL and 64 to 110 mg/dL, respectively. In the other six individuals, hospital treatment such as bicarbonate, ethanol infusion, and hemodialysis was administered. Antemortem methanol and formic acid concentrations ranged from 68 to 427 mg/dL and 37 to 91 mg/dL, respectively, whereas corresponding postmortem methanol and formic acid levels ranged from undetectable to 49 mg/dL and undetectable to 48 mg/dL, respectively. Hospital treatment of formic acid toxicity resulted in significantly reduced postmortem methanol and formic acid concentrations. Furthermore, the toxicological relevance of nine methanol-positive cases where postmortem methanol concentrations ranged from 3 to 142 mg/dL, with corresponding formic acid levels of less than 10 mg/dL, is discussed.

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Qualitative and Quantitative Analysis

Cited by 10 publications

References 34 publications

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Age-related differences in virtual histology-intravascular ultrasound findings in patients with coronary artery disease

Formic Acid and Methanol Concentrations in Death Investigations

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