volume 52, issue 2, P475-482 2013
DOI: 10.1128/jcm.01821-13
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S.- Y. G. Lin, T. C. Rodwell, T. C. Victor, E. C. Rider, L. Pham, A. Catanzaro, E. P. Desmond, G. A. Land

Abstract: cTreating extensively drug-resistant (XDR) tuberculosis (TB) is a serious challenge. Culture-based drug susceptibility testing (DST) may take 4 weeks or longer from specimen collection to the availability of results. We developed a pyrosequencing (PSQ) assay including eight subassays for the rapid identification of Mycobacterium tuberculosis complex (MTBC) and concurrent detection of mutations associated with resistance to drugs defining XDR TB. The entire procedure, from DNA extraction to the availability of…

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