Pyrethroid Resistance in the Tobacco Budworm (Lepidoptera: Noctuidae): Insecticide Bioassays and Field Monitoring

Campanhola, Clayton; Plapp, Frederick W.

doi:10.1093/jee/82.1.22

Cited by 20 publications

(12 citation statements)

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“…There are few references on the toxicity or behavioural effects of insecticides to adults, though there are many anecdotal reports of toxicity and repellence of some groups. Information is available on the contact effects of synthetic pyrethroids on adult Heliothis virescens (Fabricius) (Campanhola & Plapp 1989) and H. armigera (Daly & Fitt 1990) using the adult vial technique for monitoring resistance. A diagnostic dose of 90 µg per vial of fenvalerate was used to distinguish susceptible from resistant moths, though it was later found that the expression of resistance declined with age, so the technique was not reliable for field‐caught H. armigera (Daly 1992).…”

Section: Introductionmentioning

confidence: 99%

Development of a synthetic plant volatile‐based attracticide for female noctuid moths. III. Insecticides for adult Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Socorro

Gregg

Hawes

2010

Australian Journal of Entomology

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We investigated the efficacy of insecticides combined with a plant volatile-based attractant for Helicoverpa armigera moths, under laboratory and field conditions. In the laboratory, 16 insecticides were assessed by the level of mortality and time to incapacitate and kill moths. The proboscis extension reflex technique was used for dosing moths. The pyrethroids, bifenthrin (only when synergised by the addition of piperonyl butoxide (PBO) but not without it) and cyfluthrin (with or without PBO), endosulfan, the carbamates methomyl and thiodicarb, and spinosad killed all moths tested at rates equivalent to, or less than, those which would be applied in cover sprays targeting larvae. The shortest time to moth incapacitation and death was observed with methomyl and thiodicarb. Spinosad produced very high mortality but moths took much longer to die. The two pyrethroids gave relatively slow kills, as did endosulfan. In a field trial, four insecticides were combined with the attractant and dead moths were collected daily from 1 to 4 days after application of the attracticide on 50 m rows of cotton. Significantly more dead moths (H. armigera, H. punctigera and other noctuids) were found near the rows treated with attracticide containing methomyl compared with spinosad, fipronil and deltamethrin. For determining the impact of attracticides by recovering dead moths, quick acting insecticides are required to prevent moths flying away from the treated area to locations where they cannot be found. Methomyl and thiodicarb are suitable for this, but other insecticides especially spinosad could be used where quick action is not needed. Large numbers of moths were killed in the field trial, suggesting that attracticides for female Helicoverpa spp. moths could have significant impacts on local populations of these pests.

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Section: Introductionmentioning

confidence: 99%

Development of a synthetic plant volatile‐based attracticide for female noctuid moths. III. Insecticides for adult Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Socorro

Gregg

Hawes

2010

Australian Journal of Entomology

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“…Current methods for characterizing resistance mechanisms in field populations of the tobacco budworm include bioassays with combinations of insecticides and synergists (Raffa and Priester, 1985;Campanhola and Plapp, 1989) and biochemical assays that measure activities of enzymes associated with insecticide metabolism. However, multiple forms of these enzymes exist with differing substrate specificities and susceptibilities to inhibition by synergists (Payne, 1987;Scott, 1990;Feyereisen et al, 1991;Brown et al, 1996); thus, relevance of results from these assays is limited by the reliability of model substrates and synergists as indicators of toxicologically significant enzyme activities (Sawicki, 1987;Kirby et al, 1994;Ibrahim and Ottea, 1995).…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Pyrethroid Analogs in Pyrethroid-Susceptible and -Resistant Tobacco Budworms, Heliothis virescens (F.)

Shan

Hammer

Ottea

1997

J. Agric. Food Chem.

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The phenoxybenzyl moiety of conventional pyrethroids is a major site of oxidative metabolism in resistant tobacco budworms, Heliothis virescens (F.). In this study, this group was replaced with known P450 monooxygenase-inhibiting or oxidatively blocked groups. A variety of isomers (1R/ 1S, cis/trans) of the resulting chrysanthemates were tested as insecticides or synergists against tobacco budworms that were insecticide-susceptible (LSU) or that expressed metabolic resistance to cypermethrin (Pyr-R). A number of compounds with pentafluorophenyl, methylenedioxyphenyl, and propargyloxyphenyl groups were insecticidal, and activity was dependent on both geometric and stereochemical configuration of the acid moiety. Both trans and cis isomers of 1(R)-fenfluthrin, which contains a pentafluorophenyl group, suppressed resistance to cypermethrin in Pyr-R insects, confirming that oxidative metabolism of the phenoxybenzyl moiety is a major mechanism of resistance in this strain. Of the methylenedioxyphenyl compounds, 1R, trans, and cis isomers were toxic and partially suppressed resistance in Pyr-R larvae. Similarly, both trans and cis isomers of R(S),1(R)-propargyloxyphenyl-containing compounds were insecticidal. Finally, R(R),1(R)-cismethylenedioxyphenyl-and -propargyloxyphenyl-containing compounds were nontoxic but significantly enhanced toxicity of cypermethrin.

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“…In the US, pyrethroid resistance is monitored in the heliothine species, Heliothis virescens in the adult life-stage using the vial test (Campanhola & Plapp, 1989). The test gives reliable estimates of resistance frequencies in field populations (Micinski etal., 1991).…”

Section: Discussionmentioning

confidence: 99%

Decrease in tolerance to fenvalerate, in resistant Helicoverpa armigera after pupal diapause

Daly

Fisk

1995

Entomologia Exp Applicata

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Pyrethroid resistant and susceptible adults of Helicoverpa armigera (Lepidoptera: Noctuidae) were screened for tolerance to pyrethroids after 6 wk or 12 wk pupal diapuse. Resistant larvae and F2 larvae from a cross between resistant and susceptible parents (two replicates), were reared under conditions to induce pupal diapause. After eclosion, adults were tested in glass vials coated with the pyrethroid fenvalerate, at a dose (DD) that is known to discriminate between susceptible and heterozygous resistant individuals. In all diapause experiments, the frequency of resistance was lower in the test groups that had experienced diapause compared with the non‐diapausing control group. The underlying cause of the decline is not certain but selective mortality of resistant versus susceptible individuals could not account for all the difference in two of the three experiments ‐ tolerance to the pyrethroid, fenvalerate, is most likely to have declined either as a consequence of diapause or from the extended time of development associated with diapause. These results indicate that monitoring programs could underestimate pyrethroid resistance frequencies when using H. armigera adults emerging from diapause.

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Pyrethroid Resistance in the Tobacco Budworm (Lepidoptera: Noctuidae): Insecticide Bioassays and Field Monitoring

Cited by 20 publications

References 0 publications

Development of a synthetic plant volatile‐based attracticide for female noctuid moths. III. Insecticides for adult Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Development of a synthetic plant volatile‐based attracticide for female noctuid moths. III. Insecticides for adult Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Biological Activity of Pyrethroid Analogs in Pyrethroid-Susceptible and -Resistant Tobacco Budworms, Heliothis virescens (F.)

Decrease in tolerance to fenvalerate, in resistant Helicoverpa armigera after pupal diapause

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