Purification of SRSF1 from E. coli for Biophysical and Biochemical Assays
Zihan Zhang,
Jun Zhang
Abstract:The Ser/Arg‐rich splicing factors (SR proteins) constitute a crucial protein family in alternative splicing, comprising twelve members characterized by unique repetitive Arg‐Ser dipeptide sequences (RS) and one to two RNA‐recognition motifs (RRM). The RS regions of SR proteins undergo variable phosphorylation, resulting in unphosphorylated, partially phosphorylated, or hyper‐phosphorylated states based on functional requirements. Despite the identification of the SR protein family over 30 years ago, the purifi… Show more
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