Purification of simian immunodeficiency virus, SIVMAC251, and of its external envelope glycoprotein, gp148

Gilljam, Gustav; Siridewa, Kithmini; Hammar, Lena Marmstål

doi:10.1016/0021-9673(94)85263-4

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Nevertheless, extractions via aqueous two-phase systems with PEG, dextran, salt or polyvenyl alcohol are used for the separation of virus particles from cultivation medium. Examples for these applications are the purification or concentration of adenovirus [34], bovine leukemia virus [35], feline leukemia virus [36], and human and simian immunodeficiency virus [37].…”

Section: Extractionmentioning

confidence: 99%

Downstream processing of cell culture-derived virus particles

Wolf

Reichl

2011

Expert Review of Vaccines

118

102

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Manufacturing of cell culture-derived virus particles for vaccination and gene therapy is a rapidly growing field in the biopharmaceutical industry. The process involves a number of complex tasks and unit operations ranging from selection of host cells and virus strains for the cultivation in bioreactors to the purification and formulation of the final product. For the majority of cell culture-derived products, efforts focused on maximization of bioreactor yields, whereas design and optimization of downstream processes were often neglected. Owing to this biased focus, downstream procedures today often constitute a bottleneck in various manufacturing processes and account for the majority of the overall production costs. For efficient production methods, particularly in sight of constantly increasing economic pressure within human healthcare systems, highly productive downstream schemes have to be developed. Here, we discuss unit operations and downstream trains to purify virus particles for use as vaccines and vectors for gene therapy.

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Section: Extractionmentioning

confidence: 99%

Downstream processing of cell culture-derived virus particles

Wolf

Reichl

2011

Expert Review of Vaccines

118

102

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“…3 4,35 GNA has previously been used to purify the outer envelope glycoprotein of HIV-1, HIV-2, and SIV. 34,46 The purification procedure is a mild method that maintains both the conformation and the biological activity of the glycoprotein. Glycoprotein 125, purified from HIV-2, elicits a protective immune re-G ILLJAM ET AL.…”

Section: Discussionmentioning

confidence: 99%

Immunological Responses to Envelope Glycoprotein 120 from Subtypes of Human Immunodeficiency Virus Type 1

Gilljam¹,

Svensson²,

Ekstrom³

et al. 1999

AIDS Research and Human Retroviruses

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The outer envelope glycoprotein (gp120) from subtypes A-E of HIV-1 was purified using a specific high mannose-binding lectin, Galanthus nivalis agglutinin. All isolates were grown in peripheral blood lymphocyte cells in order to avoid selection in cell lines. A comparison of the reactivities of the envelope proteins was made using sera from patients infected with the different subtypes. In this study, the B and C subtype envelope glycoproteins showed the strongest immunological reactivity, when reacted with sera from patients infected with the same subtype of virus. On the other hand, sera of patients infected with subtype A or C virus had the strongest and broadest reactivities, to envelope glycoproteins of many subtypes. The purified gp120 proteins from all five subtypes stimulated mononuclear cells from HIV-1 (subtype B)-infected patients, indicating conserved T cell-activating epitopes. The immunological reactivities indicate that strong antigenicity does not always predict the broadest immunogenicity of an envelope glycoprotein. Glycoprotein 120 from foreign subtypes may serve to induce strong cross-reactive immune responses.

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“…The two proteins were both about 30-fold purified and reverse transcriptase activity and infectious titer were retained to a high degree. The calculated molar ratio of gp120 to p24 was twofold higher in the phase-extracted fraction than in the material pelleted by ultracentrifugation (72).…”

Section: Extraction and Purification Of Bioparticles By Polymer/polymmentioning

confidence: 97%

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

Marques

Molino

Mazzola

et al. 2013

Encyclopedia of Industrial Biotechnology

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The study of biomolecules and bioparticles has achieved great advances, thus increasing the productivity with upstream improvements. Nowadays, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from the previous years' scene. Current purification platforms will reach their physical limits at some point, creating the necessity for new approaches. This article reviews an alternative method for the extraction and purification of biomolecules/bioparticles denominated as aqueous two‐phase systems (ATPSs) . Biocompatibility and readiness to scale up are some of the characteristics of ATPSs and we also discuss its application in the biotechnology field.

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Purification of simian immunodeficiency virus, SIVMAC251, and of its external envelope glycoprotein, gp148

Cited by 8 publications

References 26 publications

Downstream processing of cell culture-derived virus particles

Downstream processing of cell culture-derived virus particles

Immunological Responses to Envelope Glycoprotein 120 from Subtypes of Human Immunodeficiency Virus Type 1

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

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