Purification of an α-l-fucoside-binding protein from Rhizobium lupini

The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation

“…Reciprocally, other adhesins from rhizobia belonging to alfalfa or soybean cross inoculation groups might be recognized also at the red clover root surface. Examples of such agglutinins are the α‐ l ‐fucoside‐binding lectin in Rhizobium lupini (Wisniewski et al , 1994) or the BJ38 protein in Bradyrhizobium japonicum (Ho et al , 1990), which is nonhomologous to RapA1 (E. J. Mongiardini et al , unpublished data). Therefore, different modes of adsorption could exist for bacteria using different adhesin sets.…”

The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation

“…Reciprocally, other adhesins from rhizobia belonging to alfalfa or soybean cross inoculation groups might be recognized also at the red clover root surface. Examples of such agglutinins are the α‐ l ‐fucoside‐binding lectin in Rhizobium lupini (Wisniewski et al , 1994) or the BJ38 protein in Bradyrhizobium japonicum (Ho et al , 1990), which is nonhomologous to RapA1 (E. J. Mongiardini et al , unpublished data). Therefore, different modes of adsorption could exist for bacteria using different adhesin sets.…”

Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors

“…Neisseria menigitidis Meningococcus meningitis, acute adrenal insufficiency P: pili (unusual in that pili contain digalactosyl-2,4diacetamido-2,4,6-trideoxyhexose) [113] P: CD66 on epithelial cells and neutrophils [114] P: glycoprotein G of RSV on the surface of the target cell (coinfection) [115] Porphyromonas (Bacteroides) gingivalis gingivitis S: D-GalNAc [116] Pseudomonas aeruginosa colonizes regions devoid of natural defenses (catheters), and causes disease in immunodeficient hosts P: pilus adhesin S: GalNAc(b1,4) in asialo-GM1 and asialo-GM2 or GM1 [117] S: salivary mucin glycopeptides (sialic acid) [118] S: lactose of glycolipids [119] Rhizobium lupini P: l-fucose-binding protein S: l-fucose [120] Staphylococcus saprophyticus urinary tract infection P: surface lectins S: blood group A (terminal GalNAc) [121] Streptococcus suis meningitis S: Gal(a1,4)Gal present in the P1 and Pk blood group antigen [122] S: N-Ac(a2 33poly-NAc-lactosamine glycans; NeuNAc(a2,3)Gal(b1,4)GlcNAc [123] Streptococci (M group A) nasopharyngitis; release of erythrogenic toxins can cause scarlet fever; can occur as secondary infection to influenza virus, and ultimate cause of fatal pneumonias in these cases P: C3, mainly C3b and iC3b on PMN [124] Streptococcus sanguis, Streptococcus sobrinus (oral cavity) part of normal flora in the upper respiratory tract: deficiency can cause disease S: sialic acid of salivary glycoproteins [125] Yersinia enterocolitica severe abdominal pain and diarrhea; can cause fatal sepsis in some cases S: Gal, GalNAc, Lac in intestinal mucin [126] Figure 3. Injury results in expression and display of E-and P-selectins on the surface of nearby endothelial cells.…”

Proteins Exported via the PrsD-PrsE Type I Secretion System and the Acidic Exopolysaccharide Are Involved in Biofilm Formation byRhizobium leguminosarum

“…One of the early stages in the formation of symbiotic root nodules on leguminous plants is the attachment of Rhizobium bacteria to the root hair tip surface. A Rhizobium Ca 2ϩ -binding protein (rhicadhesin) was shown to participate in nonspecific attachment to the roots (49), while bacterial lectins were proposed to be involved in specific attachment (22,57). It was proposed that at a later stage, bacterial aggregation and cellulose fibrils anchor the rhizobia to the root surface (49).…”

The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation

“…Reciprocally, other adhesins from rhizobia belonging to alfalfa or soybean cross inoculation groups might be recognized also at the red clover root surface. Examples of such agglutinins are the α‐ l ‐fucoside‐binding lectin in Rhizobium lupini (Wisniewski et al , 1994) or the BJ38 protein in Bradyrhizobium japonicum (Ho et al , 1990), which is nonhomologous to RapA1 (E. J. Mongiardini et al , unpublished data). Therefore, different modes of adsorption could exist for bacteria using different adhesin sets.…”

Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors

“…Neisseria menigitidis Meningococcus meningitis, acute adrenal insufficiency P: pili (unusual in that pili contain digalactosyl-2,4diacetamido-2,4,6-trideoxyhexose) [113] P: CD66 on epithelial cells and neutrophils [114] P: glycoprotein G of RSV on the surface of the target cell (coinfection) [115] Porphyromonas (Bacteroides) gingivalis gingivitis S: D-GalNAc [116] Pseudomonas aeruginosa colonizes regions devoid of natural defenses (catheters), and causes disease in immunodeficient hosts P: pilus adhesin S: GalNAc(b1,4) in asialo-GM1 and asialo-GM2 or GM1 [117] S: salivary mucin glycopeptides (sialic acid) [118] S: lactose of glycolipids [119] Rhizobium lupini P: l-fucose-binding protein S: l-fucose [120] Staphylococcus saprophyticus urinary tract infection P: surface lectins S: blood group A (terminal GalNAc) [121] Streptococcus suis meningitis S: Gal(a1,4)Gal present in the P1 and Pk blood group antigen [122] S: N-Ac(a2 33poly-NAc-lactosamine glycans; NeuNAc(a2,3)Gal(b1,4)GlcNAc [123] Streptococci (M group A) nasopharyngitis; release of erythrogenic toxins can cause scarlet fever; can occur as secondary infection to influenza virus, and ultimate cause of fatal pneumonias in these cases P: C3, mainly C3b and iC3b on PMN [124] Streptococcus sanguis, Streptococcus sobrinus (oral cavity) part of normal flora in the upper respiratory tract: deficiency can cause disease S: sialic acid of salivary glycoproteins [125] Yersinia enterocolitica severe abdominal pain and diarrhea; can cause fatal sepsis in some cases S: Gal, GalNAc, Lac in intestinal mucin [126] Figure 3. Injury results in expression and display of E-and P-selectins on the surface of nearby endothelial cells.…”

Proteins Exported via the PrsD-PrsE Type I Secretion System and the Acidic Exopolysaccharide Are Involved in Biofilm Formation byRhizobium leguminosarum

“…One of the early stages in the formation of symbiotic root nodules on leguminous plants is the attachment of Rhizobium bacteria to the root hair tip surface. A Rhizobium Ca 2ϩ -binding protein (rhicadhesin) was shown to participate in nonspecific attachment to the roots (49), while bacterial lectins were proposed to be involved in specific attachment (22,57). It was proposed that at a later stage, bacterial aggregation and cellulose fibrils anchor the rhizobia to the root surface (49).…”

The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation

“…Reciprocally, other adhesins from rhizobia belonging to alfalfa or soybean cross inoculation groups might be recognized also at the red clover root surface. Examples of such agglutinins are the α‐ l ‐fucoside‐binding lectin in Rhizobium lupini (Wisniewski et al , 1994) or the BJ38 protein in Bradyrhizobium japonicum (Ho et al , 1990), which is nonhomologous to RapA1 (E. J. Mongiardini et al , unpublished data). Therefore, different modes of adsorption could exist for bacteria using different adhesin sets.…”

Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors

“…Neisseria menigitidis Meningococcus meningitis, acute adrenal insufficiency P: pili (unusual in that pili contain digalactosyl-2,4diacetamido-2,4,6-trideoxyhexose) [113] P: CD66 on epithelial cells and neutrophils [114] P: glycoprotein G of RSV on the surface of the target cell (coinfection) [115] Porphyromonas (Bacteroides) gingivalis gingivitis S: D-GalNAc [116] Pseudomonas aeruginosa colonizes regions devoid of natural defenses (catheters), and causes disease in immunodeficient hosts P: pilus adhesin S: GalNAc(b1,4) in asialo-GM1 and asialo-GM2 or GM1 [117] S: salivary mucin glycopeptides (sialic acid) [118] S: lactose of glycolipids [119] Rhizobium lupini P: l-fucose-binding protein S: l-fucose [120] Staphylococcus saprophyticus urinary tract infection P: surface lectins S: blood group A (terminal GalNAc) [121] Streptococcus suis meningitis S: Gal(a1,4)Gal present in the P1 and Pk blood group antigen [122] S: N-Ac(a2 33poly-NAc-lactosamine glycans; NeuNAc(a2,3)Gal(b1,4)GlcNAc [123] Streptococci (M group A) nasopharyngitis; release of erythrogenic toxins can cause scarlet fever; can occur as secondary infection to influenza virus, and ultimate cause of fatal pneumonias in these cases P: C3, mainly C3b and iC3b on PMN [124] Streptococcus sanguis, Streptococcus sobrinus (oral cavity) part of normal flora in the upper respiratory tract: deficiency can cause disease S: sialic acid of salivary glycoproteins [125] Yersinia enterocolitica severe abdominal pain and diarrhea; can cause fatal sepsis in some cases S: Gal, GalNAc, Lac in intestinal mucin [126] Figure 3. Injury results in expression and display of E-and P-selectins on the surface of nearby endothelial cells.…”

Proteins Exported via the PrsD-PrsE Type I Secretion System and the Acidic Exopolysaccharide Are Involved in Biofilm Formation byRhizobium leguminosarum

“…One of the early stages in the formation of symbiotic root nodules on leguminous plants is the attachment of Rhizobium bacteria to the root hair tip surface. A Rhizobium Ca 2ϩ -binding protein (rhicadhesin) was shown to participate in nonspecific attachment to the roots (49), while bacterial lectins were proposed to be involved in specific attachment (22,57). It was proposed that at a later stage, bacterial aggregation and cellulose fibrils anchor the rhizobia to the root surface (49).…”