2012
DOI: 10.1107/s1744309112000115
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: A triclosan-resistant flavoprotein termed FabK is the sole enoyl-acyl carrier protein reductase in Streptococcus pneumoniae and Streptococcus mutans. In this study, FabK from S. mutans strain UA159 was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.40 Å resolution using a synchrotron-radiation source. The crystal belonged to space group P6(2), with unit-cell parameters a = b = 105.79, c = 44.15 Å. The asymmetric unit contained one molecule, with a correspondi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
5
0

Year Published

2013
2013
2024
2024

Publication Types

Select...
5

Relationship

0
5

Authors

Journals

citations
Cited by 5 publications
(5 citation statements)
references
References 18 publications
0
5
0
Order By: Relevance
“…Docking studies suggest the active compounds were binding at the 4FS3 active site through amino acid residues Tyr157 and Phe96. An alternate enoyl reductase encoding gene, FabK, is present in some bacterial species, including streptococcus . ,, FabK is also involved in fatty acid synthesis . At this stage, it is difficult to justify whether the activity of the compounds is solely due to FabI inhibition or an additional target is also involved, and this should be validated by enzyme studies, which are not conducted here.…”
Section: Resultsmentioning
confidence: 99%
“…Docking studies suggest the active compounds were binding at the 4FS3 active site through amino acid residues Tyr157 and Phe96. An alternate enoyl reductase encoding gene, FabK, is present in some bacterial species, including streptococcus . ,, FabK is also involved in fatty acid synthesis . At this stage, it is difficult to justify whether the activity of the compounds is solely due to FabI inhibition or an additional target is also involved, and this should be validated by enzyme studies, which are not conducted here.…”
Section: Resultsmentioning
confidence: 99%
“…Possibly, TCS is a multi-target inhibitor for S. mutans, which lack a triclosan-sensitive Fabi enoyl-ACP reductase, and that inhibition of glycolysis in dental plaque biofilms, in which TCS is retained after initial or repeated exposure, would reduce cariogenicity 16 . Besides, other studies report the relationship of TCS and S. mutans, where possibly the gene called FabK, an isoenzyme present in the bacterial cell membrane, may be the target of the TCS 17,18 .…”
Section: Discussionmentioning
confidence: 99%
“…MCAT has received much attention as a possible antibiotic target because FAS II differs from FAS I in mammals, and great progress has been made over the last decade with regard to MCAT in pathogenic bacteria. Analyses of the crystal structures of MCAT from Escherichia coli [17], Streptomyces coelicolor [3], H. pylori [18], M. tuberculosis [19], Staphylococcus aureus [20], Streptococcus mutans strain UA159 [21], and Xanthomonas oryzae pv. Oryzae [22] have suggested that MCAT is one of the most promising targets for antibacterial agent discovery.…”
Section: Activity Of Mcat From Cultivars With Different Oil Contentmentioning
confidence: 99%