Purification and structural characterisation of lipid transfer protein from red wine and grapes

Jaeckels, Nadine; Rosfa, Susanne; Schild, Hansjoerg; Decker, Heinz; Wigand, Petra

doi:10.1016/j.foodchem.2012.09.113

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…Capillary liquid chromatography of tryptic peptides was performed with a Waters NanoAcquity UPLC system equipped with a 75 lm Â 150 mm HSS-T3 reversed phase column and a 2.6 ll PEEKSIL-sample loop (SGE, Darmstadt, Germany) as described before (Jaeckels et al, 2013).…”

Section: Uplc Configurationmentioning

confidence: 99%

“…All analyses were performed using positive mode ESI using a NanoLockSpray source as described before (Jaeckels et al, 2013).…”

Section: Mass Spectrometer Configurationmentioning

confidence: 99%

“…(Waters). Protein identifications were assigned by searching a database containing all known protein sequences from Vitis vinifera, Saccharomyces cerevisiae and known possible contaminants (trypsin, human keratins) with the precursor and fragmentation data afforded by the LCMSMS acquisition method as described before (Jaeckels et al, 2013).…”

Section: Data Processing and Protein Identificationmentioning

confidence: 99%

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Polyphenoloxidase from Riesling and Dornfelder wine grapes (Vitis vinifera) is a tyrosinase

et al. 2015

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Section: Uplc Configurationmentioning

confidence: 99%

“…All analyses were performed using positive mode ESI using a NanoLockSpray source as described before (Jaeckels et al, 2013).…”

Section: Mass Spectrometer Configurationmentioning

confidence: 99%

Section: Data Processing and Protein Identificationmentioning

confidence: 99%

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Polyphenoloxidase from Riesling and Dornfelder wine grapes (Vitis vinifera) is a tyrosinase

et al. 2015

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“…Protein identifications were assigned by searching a custom compiled database containing open reading frames obtained from transcriptome sequencing of W. anomalus [19] for gel bands derived from W. anomalus and all available (212.809) RefSeq database entries from the order of Saccharomycetales for gel bands derived from Metschnikowia pulcherrima. Sequence databases were supplemented by known possible contaminants (trypsin, human keratins) based on the precursor and fragmentation data afforded by the LC-MS/MS acquisition method as described before [20]. The false discovery rate (FDR) for peptide and protein identification was assessed searching a reverse database generated automatically in PLGS.…”

Section: Identification Of Yeast Exoenzymesmentioning

confidence: 99%

“…Proteins from a German Riesling wine (vintage 2012) were prepared by dialysis and lyophilisation as recently described by Jaeckels et al [20]. The proteolytic activity against isolated wine proteins and bovine serum albumin (Sigma-Aldrich, Munich, Germany) were tested after incubation at 20 • C in sodium tartrate buffer (pH 3.5) and subsequently in an actual German Riesling wine (vintage 2014, 11.5% v/v alcohol).…”

Section: Degradation Experimentsmentioning

confidence: 99%

Purification and Properties of Yeast Proteases Secreted by Wickerhamomyces anomalus 227 and Metschnikovia pulcherrima 446 during Growth in a White Grape Juice

et al. 2016

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Aspartic proteases are of significant importance for medicine and biotechnology. In spite of sufficient evidence that many non-Saccharomyces yeasts produce extracellular proteases, previous research has focused on the enzymes of Candida species because of their role as virulence factors. Nowadays, there is also increasing interest for their applications in industrial processes, mainly because of their activities at low pH values. Here, we report the features of new acid proteases isolated from wine-relevant yeasts Metschnikovia pulcherrima and Wickerhamomyces anomalus. To our knowledge, this is the first detailed description of such an enzyme derived from strains of W. anomalus. Deviating to most former studies, we could demonstrate that the yeasts produce these enzymes in a natural substrate (grape juice) during the active growth phase. The enzymes were purified from concentrated grape juice by preparative isoelectric focusing. Biochemical data (maximum activity at ≈ pH 3.0, inhibition by pepstatin A) classify them as aspartic proteases. For W. anomalus 227, this assumption was confirmed by the protein sequence of WaAPR1 determined by LC-MS/MS. The sequence revealed a signal peptide for secretion, as well as a peptidase A1 domain with two aspartate residues in the active site. The enzyme has a calculated molecular mass of 47 kDa and an isolelectric point of 4.11.

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Separation and characterization of sucrose esters from Oriental tobacco leaves using accelerated solvent extraction followed by SPE coupled to HPLC with ion‐trap MS detection

Zhu

Feng

Yang

et al. 2013

J of Separation Science

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Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic-lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid-solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves.

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Purification and structural characterisation of lipid transfer protein from red wine and grapes

Cited by 16 publications

References 28 publications

Polyphenoloxidase from Riesling and Dornfelder wine grapes (Vitis vinifera) is a tyrosinase

Polyphenoloxidase from Riesling and Dornfelder wine grapes (Vitis vinifera) is a tyrosinase

Purification and Properties of Yeast Proteases Secreted by Wickerhamomyces anomalus 227 and Metschnikovia pulcherrima 446 during Growth in a White Grape Juice

Separation and characterization of sucrose esters from Oriental tobacco leaves using accelerated solvent extraction followed by SPE coupled to HPLC with ion‐trap MS detection

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