Purification and some properties of peroxidases of rat bone marrow

Bone Marrow Peroxidases of Spontaneously Hypertensive Rats

“…As reported previously, rat bone marrow con tains two peroxidases, namely, myeloperoxi dase and eosinophil peroxidase (9). Because KI is a good substrate for eosinophil peroxi dase and the activity of myeloperoxidase using KI as the substrate is very low, the ac tivity toward KI is thought to be due to eosinophil peroxidase, but not myeloperoxi dase (9).…”

“…As reported previously, rat bone marrow con tains two peroxidases, namely, myeloperoxi dase and eosinophil peroxidase (9). Because KI is a good substrate for eosinophil peroxi dase and the activity of myeloperoxidase using KI as the substrate is very low, the ac tivity toward KI is thought to be due to eosinophil peroxidase, but not myeloperoxi dase (9). When KI was used as the electron donor, the peroxidase activity of male WKY was higher than that of SHR and SHR-SP, suggesting that the bone marrow of male SH R and SHR-SP contain a lower activity of eosinophil peroxidase compared with WKY.…”

“…The inactivation of bone marrow peroxidase was accompanied by a change in the heme structure (8). In ad dition, it was demonstrated that rat bone marrow contains two peroxidases, namely myeloperoxidase and eosinophil peroxidase, and both peroxidases were homogeneously purified from rat bone marrow (9). The characteristics of myeloperoxidase including the catalytic activity, subunit composition and the structure surrounding the heme were dif ferent from those of eosinophil peroxidase (9).…”

“…In ad dition, it was demonstrated that rat bone marrow contains two peroxidases, namely myeloperoxidase and eosinophil peroxidase, and both peroxidases were homogeneously purified from rat bone marrow (9). The characteristics of myeloperoxidase including the catalytic activity, subunit composition and the structure surrounding the heme were dif ferent from those of eosinophil peroxidase (9). Although these evidences indicate that the two peroxidases play different roles in the bone marrow, little is known about the physiological and pathophysiological func tions of the peroxidases in this tissue.…”

“…Assay of the peroxidase activity: Prepara tion of bone marrow cells of rats were carried out as described previously (9). Briefly, the 105,000xg pellet was solubilized with 10 mM potassium phosphate buffer (pH 7.0) con taining 0.3% CETAB.…”