volume 16, issue 4, P281-285 1994
DOI: 10.1016/0141-0229(94)90167-8
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M. Fisher, S. Harbron, H.J. Eggelte, B.R. Rabin

Abstract: Nonradioactive immunoassays incorporating an element of amplification in their detection system require the use of components that are highly purified. Flavin adenine dinucleotide-3'-phosphate (FADP) is the primary substrate used in such an amplification assay. For incorporation into a simple, single-pot assay system, the concentration of contaminating flavin adenine dinucleotide (a prosthetic group for the enzyme D-aminoacid oxidase used in the amplification cascade assay) in this primary substrate must be mi…

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