Purification and renal effects of phospholipase A2 isolated from Bothrops insularis venom

Braga, Marcus Davis Machado; Martins, Alice Maria Costa; Alves, Claudênio Diógenes; Menezes, Dalgimar Beserra de; Martins, René Duarte; Barbosa, Paula; Oliveira, Isadora M. de; Toyama, Marcos Hikari; Toyama, Daniela O.; Filho, Eduardo Brito dos Santos Diz; Fagundes, Fábio H. R.; Fonteles, M. C.; Monteiro, Helena Serra Azul

doi:10.1016/j.toxicon.2007.08.017

Cited by 16 publications

(4 citation statements)

References 18 publications

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“…AKI is 10 times more common in crotalic accidents than in bothropic, but the incidences are similar due to the greater number of accidents caused by the Bothrops genus [ 31 , 36 ]. Venom toxins have direct renal action, changing their structure [ 30 , 38 – 40 ] and physiology [ 41 ] due to capillary vulnerability. Moreover, Acute Tubular Necrosis (ATN) is the most common cause of AKI in snakebite envenomation [ 38 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Predicting acute renal failure in Bothrops snakebite patients in a tertiary reference center, Western Brazilian Amazon

et al. 2018

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Acute Kidney Injury (AKI) is the main systemic complication and cause of death in viperid envenomation. Although there are hypotheses for the development of AKI, the mechanisms involved are still not established. The aim of this study was to evaluate the clinical-laboratorial-epidemiological factors associated with AKI in victims of Bothrops sp envenomation. This is an observational study carried out at the Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. AKI was defined according to the guidelines of the Acute Kidney Injury Network (AKIN). Among the 186 patients evaluated, AKI was observed in 24 (12.9%) after 48 hours of admission. Stage I was present in 17 (70.8%) patients, II in 3 (12.5%) and III in 4 (16.7%). Epidemiological characterization showed predominance of men, occurrence in rural areas, aged between 16–60 years, feet as the most affected anatomical region, and time to medical assistance less than 3 hours. Hypertension and diabetes were the comorbidities identified. Most of the accidents were classified as moderate, and clinical manifestations included severe pain, mild edema, local bleeding and headache. Laboratory results showed blood uncoagulability, hypofibrinogenemia, leukocytosis, increase of creatine kinase, and high lactate dehydrogenase levels. Multivariate analysis showed an association with high LDH levels [AOR = 1.01 (95% CI = 1.01–1.01, p<0.002)], local bleeding [AOR = 0.13 (95%CI = 0.027–0.59, p<0.009)], and the presence of comorbidities [AOR = 60.96 (95%CI = 9.69–383.30; p<0.000)]. Herein, laboratory markers such as high LDH levels along with local bleeding and comorbidities may aid in the diagnosis of AKI.

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Section: Introductionmentioning

confidence: 99%

Predicting acute renal failure in Bothrops snakebite patients in a tertiary reference center, Western Brazilian Amazon

et al. 2018

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“…PLA 2 s have a pivotal role in inflammation by activating arachidonic acid that leads to generation of eicosanoids (prostaglandins and leukotrienes). They also stimulate the hypothalamicpituitary-adrenal axis to produce adrenocorticotropic hormone, corticosteroids, vasopressin and acute phase proteins; as well as cause local manifestations at the bite site and hemodynamic changes [43,[45][46][47][48]. SVSPs are highly expressed in kidneys.…”

Section: Bothrops Venommentioning

confidence: 99%

Clinical assessment and pathophysiology of Bothrops venom-related acute kidney injury: a scoping review

Albuquerque

Paiva

Martins

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Bothrops are one of the most common medically important snakes found in Latin America. Its venom is predominantly hemotoxic and proteolytic, which means that local lesion (edema and redness) and hemorrhagic symptoms are recurrent in envenoming by this snake. Although hemorrhage is usually the major cause of death, snakebite-related acute kidney injury is another potentially fatal clinical complication that may lead to chronic kidney disease. The present review highlights the main studies on Bothrops venom-related acute kidney injury, including observational, cross-sectional, case-control and cohort human studies available up to December 2019. The following descriptors were used according to Medical Subject Headings (MeSH): on Medline/Pubmed and Google Scholar "acute kidney injury" or "kidney disease" and "Bothrops"; on Lilacs and SciELO "kidney disease" or "acute kidney injury" and "Bothrops". Newcastle-Ottawa quality assessment scale was used to appraise the quality of the cross-sectional and cohort studies included. The selection of more severe patients who looked for health care units and tertiary centers is a risk of bias. Due to the methodological heterogeneity of the studies, a critical analysis of the results was performed based on the hypothesis that the design of the included studies influences the incidence of acute kidney injury. Fifteen human studies (total participants 4624) were included according to stablished criteria. The coagulation abnormalities (hemorrhagic symptoms, abnormal fibrinogen and activated partial thromboplastin time) were associated with acute kidney injury in

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“…The pathogenesis of AKI remains poorly described, but factors such as bleeding, inflammatory processes, fibrin deposition in the renal tubules, immune complexes formation, and the venom's direct action may be related [ 18 , 21 – 23 ]. Studies with different Bothrops species have shown that BaV can lead to renal damage and proximal tubular damage after the bite [ 22 , 24 ]. In addition, cytokines can be released by leukocytes and renal tubular cells after cell injury and are important components of the initiation and extension of inflammation in AKI [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

CCL-2 and CXCL-8: Potential Prognostic Biomarkers of Acute Kidney Injury after a Bothrops atrox Snakebite

Neves

Ibiapina

Sachett

et al. 2022

Mediators of Inflammation

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In the Brazilian Amazon, the snake Bothrops atrox is the primary cause of snakebites. B. atrox (BaV) venom can cause systemic pathophysiological changes such as acute kidney injury (AKI), which leads to the production of chemokines and cytokines in response to the envenomation. These soluble immunological molecules act by modulating the inflammatory response; however, the mechanisms associated with the development of AKI are still poorly understood. Here, we characterize the profile of these soluble immunological molecules as possible predictive biomarkers of the development of AKI. The study involved 34 patients who had been victims of snakebites by Bothrops sp. These were categorized into two groups according to the development of AKI (AKI(-)/AKI(+)), using healthy donors as the control (HD). Peripheral blood samples were collected at three-time points: before antivenom administration (T0) and at 24 and 48 hours after antivenom (T1 and T2, respectively). The soluble immunological molecules (CXCL-8, CCL-5, CXCL-9, CCL-2, CXCL-10, IL-6, TNF, IL-2, IL-10, IFN-γ, IL-4, and IL-17A) were quantified using cytometric bead array. Our results demonstrated an increase in CXCL-9, CXCL-10, IL-6, IL-2, IL-10, and IL-17A molecules in the groups of patients who suffered Bothrops snakebites (AKI(-) and AKI(+)) before antivenom administration, when compared to HD. In the AKI(+) group, levels of CXCL-8 and CCL-2 molecules were elevated on admission and progressively decreased during the clinical evolution of patients after antivenom administration. In addition, in the signature analysis, these were produced exclusively by the group AKI(+) at T0. Thus, these chemokines may be related to the initiation and extension of AKI after envenomation by Bothrops and present themselves as two potential biomarkers of AKI at T0.

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Purification and renal effects of phospholipase A2 isolated from Bothrops insularis venom

Cited by 16 publications

References 18 publications

Predicting acute renal failure in Bothrops snakebite patients in a tertiary reference center, Western Brazilian Amazon

Predicting acute renal failure in Bothrops snakebite patients in a tertiary reference center, Western Brazilian Amazon

Clinical assessment and pathophysiology of Bothrops venom-related acute kidney injury: a scoping review

CCL-2 and CXCL-8: Potential Prognostic Biomarkers of Acute Kidney Injury after a Bothrops atrox Snakebite

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