Purification and properties of chitosanase from a mutant of Bacillus subtilis IMR-NK1

Chiang, Chui-Liang; Chang, Chih-Wei; Sung, Hong Gun

doi:10.1016/s0141-0229(02)00275-2

Cited by 64 publications

(60 citation statements)

References 32 publications

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“…3.2.1.132) hydrolyze the β-1,4-glycosidic linkage of chitosan, which is a polysaccharide consisting mainly of D-glucosamine with a variable content of N-acetyl-D-glucosamine (Piza et al, 1999). Preparation of chitooligosaccharides with chitosanase is preferred to chemical methods because it allows production of a specific type of chitooligosaccharides (Hsiao et al, 2008), but the catalytic features of chitosanase from different microorganisms lead to different polymerization degrees (Chiang et al, 2003). For example, chitotriose was obtained as the major product by hydrolyzing chitosan with chitosanase from Gongronella sp.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Cheng

Wang

Hong

2012

Braz. J. Chem. Eng.

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-The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO 4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH 4 ) 2 SO 4 , 8 g/L urea, 1 g/L NH 4 Cl, 3 g/L KH 2 PO 4 , 1 g/L FeSO 4 ·7H 2 O, 2 g/L ZnSO 4 ·7H 2 O, 2 g/L CaCl 2 ·6H 2 O, 2 g/L MnSO 4 ·H 2 O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.

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Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Cheng

Wang

Hong

2012

Braz. J. Chem. Eng.

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“…The strain showed the higher activity of 2.198 mg/ml with 1.5% on the 5 th day. No growth was seen at 2.0% chitosan due to high viscosity of the medium with limited oxygen availability and also high concentration of chitosan inhibited the growth of the organisms [24,25]. …”

Section: Effect Of Time and Substrate For Chitosanase Productionmentioning

confidence: 99%

Production and Characterization of Partially Purified Chitosanase Enzyme of <i>Bacillus cereus</i> A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide

Vanathi

Fernández

Shanmugapriya

et al. 2015

FoodSci: Indian Journal of Research in Food Science and Nutrition

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“…Many chitinase-producing strains such as B. cereus 65 , B. cereus YQ308 (Pleban et al, 1997), B. subtilis W-118 (Wang et al, 2006), B. subtilis IMR-NK1 (Chiang et al, 2003) and A. hydrophila JP101 (Wu et al, 2001) produce a single chitinase in the cell supernatant. However, preliminary results from the electrophoretic methods reported in this study should be confirmed by the total purification of chitinase by other methods such as chromatography and analytical ultracentrifugation to estimate the molecular mass exactly and to check whether the chitinase has two or several isoforms.…”

Section: Antifungal Chitinase Characterizationmentioning

confidence: 99%

In vivo and in vitro evaluation of antifungal activities from a halotolerant Bacillus subtilis strain J9

Essghaier¹,

Hedi²,

Hajlaoui³

et al. 2012

Afr. J. Microbiol. Res.

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In this study, we aimed to identify the antifungal activities of the halotolerant Bacillus subtilis strain J9 under different carbon sources and salinity conditions in vitro and in vivo under field conditions on strawberry. The strain J9 of B. subtilis was able to reduce grey mold disease on strawberry under field conditions with percentage reduction ranging from 46.3 to 44.3% which was comparable to those of chemical fungicides respectively after treatments of 8 and 40 days. Therefore, the strain J9 may be a good candidate for biological control. The strain J9 was able to produce high antifungal enzymes inducible by the pathogen; chitinase (102 U/ml), protease (14.5 U/ml) and laminarinase (0.74 U/ml). Furthermore, high halotolerant chitinase was detected in the culture supernatant under 0 to 25% NaCl (w/v). Interestingly, we described distinguishable characteristics of high activity and stability at different pH, temperatures of B. subtilis J9 chitinase. The analysis of the amino acid sequences coding to the putative catalytic domain from enzyme highlighted its relation to those of glycosyl hydrolase family 18.

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Purification and properties of chitosanase from a mutant of Bacillus subtilis IMR-NK1

Cited by 64 publications

References 32 publications

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Production and Characterization of Partially Purified Chitosanase Enzyme of <i>Bacillus cereus</i> A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide

In vivo and in vitro evaluation of antifungal activities from a halotolerant Bacillus subtilis strain J9

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