Purification and Characterization of Membrane-bound Aldehyde Dehydrogenase fromGluconobacter suboxydans

Production of Glyceric Acid byGluconobactersp. NBRC3259 Using Raw Glycerol

“…Until now, concerning the production mechanisms of GA from glycerol by acetic acid bacteria at the molecular level, no reports have been published. Adachi et al 17,18) reported that purified membrane-bound alcohol dehydrogenase and membrane-bound aldehyde dehydrogenase from G. suboxydans did not exhibit specific activities toward glycerol and DL-glyceraldehyde respectively. Therefore, to elucidate the production mechanisms of D-GA and of L-GA, it is important to purify and characterize the enzymes involved in GA production.…”

Enzyme systems dehydrogenating ethanol and acetaldehyde in crude cell-free extracts of Acetobacter aceti LMD 23.1

“…Besides dehydrogenases which required NADP or NAD as coenzymes enzyme systems dehydrogenating ethanol [1,2] and acetaldehyde [3] not requiring coenzymes have been detected in the membrane fraction of several Acetobacter and Gluconobacter species. Ferricyanide and dyes can serve in vitro as the electron acceptors of these dehydrogenases [1][2][3].…”

Purification and Characterization of Aldehyde Dehydrogenase ofAcetobacter aceti

“…The enzyme showed the typical absorption spectrum of c-type cytochrome, a similar to that seen in the enzyme from G. suboxydans. 3 ) The oxidized enzyme had an absorption maximum at 411 nm, and reduction of the enzyme with sodium dithionite resulted in absorption maxima at 551, 523 and 417 nm, as shown in Fig. 2.…”

“…They are typical membrane-bound enzymes and solubilized from the membrane with detergent. ADH from Acetobacter aceti ' ) and Gluconobacter suboxydaml> and ALDH from G. suboxydans 3 ) have been purified to homogeneity and characterized enzymatically. They have been purified as an enzyme complex composed of dehydrogenase and c-type cytochrome.…”