Purification and characterization of fertility-associated antigen (FAA) in bovine seminal fluid

Characterization of fertility-associated antigens in seminal fluid and their relationship with vital sperm function tests vis-à-vis fertility of breeding buffalo bulls

“…However, all proteins were not recognized by anti-FA-1 in seminal plasma and frozen-thawed sperm extracts of each bull. These findings are in agreement with the observations of McCauley et al (1999) who named the proteins with molecular weight of 18, 31, 33, 48 and 55 kDa as fertilityassociated antigen-5-complex (FAA-5-complex) in sperm extracts and recognized them as a diagnostic indicator of semen fertility. Similar studies (Roncoletta et al 2006) in seminal plasma of Nelore bulls also demonstrated six FAA having molecular weight from 14-65 kDa (14, 18, 33, 45, 60 and 65).…”

Characterization of fertility-associated antigens in seminal fluid and their relationship with vital sperm function tests vis-à-vis fertility of breeding buffalo bulls

“…However, all proteins were not recognized by anti-FA-1 in seminal plasma and frozen-thawed sperm extracts of each bull. These findings are in agreement with the observations of McCauley et al (1999) who named the proteins with molecular weight of 18, 31, 33, 48 and 55 kDa as fertilityassociated antigen-5-complex (FAA-5-complex) in sperm extracts and recognized them as a diagnostic indicator of semen fertility. Similar studies (Roncoletta et al 2006) in seminal plasma of Nelore bulls also demonstrated six FAA having molecular weight from 14-65 kDa (14, 18, 33, 45, 60 and 65).…”

Seminal DNase Frees Spermatozoa Entangled in Neutrophil Extracellular Traps

“…This is supported by the fact that the stallion with no DNase activity is a known carrier of severe combined immunodeficiency, and his fertility with repeated inseminations per estrus is 10% lower than a single insemination. Because the semen content of DNase differs among stallions, as shown here and as previously reported in bulls [20], screening of males for DNase deficiencies may eliminate the need for more expensive reproductive procedures.…”

Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-2 and -5 in Equine Seminal Plasma: Association with Sperm Characteristics and Fertility1

“…In addition, heparin-binding proteins found in bovine seminal plasma have been linked to fertility of bulls [58]. Heparin-binding proteins are secreted by the accessory sex glands and modulate heparin-sperm activity [59]. Although the role of the seminal plasma IGF proteins on sperm function of humans or domestic species has not been fully elucidated, work from this study and others suggests that these proteins are also linked to sperm function.…”

Characterization of fertility-associated antigens in seminal fluid and their relationship with vital sperm function tests vis-à-vis fertility of breeding buffalo bulls

“…However, all proteins were not recognized by anti-FA-1 in seminal plasma and frozen-thawed sperm extracts of each bull. These findings are in agreement with the observations of McCauley et al (1999) who named the proteins with molecular weight of 18, 31, 33, 48 and 55 kDa as fertilityassociated antigen-5-complex (FAA-5-complex) in sperm extracts and recognized them as a diagnostic indicator of semen fertility. Similar studies (Roncoletta et al 2006) in seminal plasma of Nelore bulls also demonstrated six FAA having molecular weight from 14-65 kDa (14, 18, 33, 45, 60 and 65).…”

Seminal DNase Frees Spermatozoa Entangled in Neutrophil Extracellular Traps

“…This is supported by the fact that the stallion with no DNase activity is a known carrier of severe combined immunodeficiency, and his fertility with repeated inseminations per estrus is 10% lower than a single insemination. Because the semen content of DNase differs among stallions, as shown here and as previously reported in bulls [20], screening of males for DNase deficiencies may eliminate the need for more expensive reproductive procedures.…”

Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-2 and -5 in Equine Seminal Plasma: Association with Sperm Characteristics and Fertility1

“…In addition, heparin-binding proteins found in bovine seminal plasma have been linked to fertility of bulls [58]. Heparin-binding proteins are secreted by the accessory sex glands and modulate heparin-sperm activity [59]. Although the role of the seminal plasma IGF proteins on sperm function of humans or domestic species has not been fully elucidated, work from this study and others suggests that these proteins are also linked to sperm function.…”

Characterization of fertility-associated antigens in seminal fluid and their relationship with vital sperm function tests vis-à-vis fertility of breeding buffalo bulls

“…However, all proteins were not recognized by anti-FA-1 in seminal plasma and frozen-thawed sperm extracts of each bull. These findings are in agreement with the observations of McCauley et al (1999) who named the proteins with molecular weight of 18, 31, 33, 48 and 55 kDa as fertilityassociated antigen-5-complex (FAA-5-complex) in sperm extracts and recognized them as a diagnostic indicator of semen fertility. Similar studies (Roncoletta et al 2006) in seminal plasma of Nelore bulls also demonstrated six FAA having molecular weight from 14-65 kDa (14, 18, 33, 45, 60 and 65).…”

Seminal DNase Frees Spermatozoa Entangled in Neutrophil Extracellular Traps

“…This is supported by the fact that the stallion with no DNase activity is a known carrier of severe combined immunodeficiency, and his fertility with repeated inseminations per estrus is 10% lower than a single insemination. Because the semen content of DNase differs among stallions, as shown here and as previously reported in bulls [20], screening of males for DNase deficiencies may eliminate the need for more expensive reproductive procedures.…”

Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-2 and -5 in Equine Seminal Plasma: Association with Sperm Characteristics and Fertility1

“…In addition, heparin-binding proteins found in bovine seminal plasma have been linked to fertility of bulls [58]. Heparin-binding proteins are secreted by the accessory sex glands and modulate heparin-sperm activity [59]. Although the role of the seminal plasma IGF proteins on sperm function of humans or domestic species has not been fully elucidated, work from this study and others suggests that these proteins are also linked to sperm function.…”