volume 18, issue 5, P383-387 1996
DOI: 10.1016/0141-0229(95)00154-9
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Shuen-Fuh Lin, Chien-Ming Chiou, Ying-Chieh Tsai

Abstract: A novel extracellular glycerol oxidase was purified 39-fold from wheat bran culture of a soil-isolated Penicillium strain TS-622 with an overall yield of 3%. The addition of Triton X-100 into the extraction buffer improved the extraction yield by 90 times, indicating that the enzyme is bound to the cell surface. The molecular weight of this enzyme was 400,000 as determined by size-exclusion high-performance liquid chromatography. The optimum pH was from 6 to 7 and the optimum temperature was 45 degrees C. This…

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