Purification and characterization of a thermotolerant laccase isoform in Trametes trogii strain and its potential in dye decolorization

An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01

“…Laccase from P. sanguineus BRFM 66 also possessed considerably high decolorization activity toward Anthraquinonic Dye Poly R-478 (33%) and Heterocyclic Dye Azure B (21%) after 52 h of incubation [50]. Laccase from T. trogii was effective to decolorize Malachite Green, Bromophenol Blue, Crystal Violet, and Acid Red without the addition of redox mediators [47]. In the present study, the two dyes Bromothymol Blue and Malachite Green share very close structure with three benzene rings.…”

“…F. solani MAS2 laccase needed incubation time of 40 days and achieved the maximum activity at day 35 of 575 mU/mg protein [46]. Optimization of laccase production of Trametes trogii S0301 by response surface methodology revealed that the maximum laccase activity of 122.9 U/mL was obtained at day 9 with phenol, CuSO 4 and PEG 4000 as inducers [47]. The high laccase yield and short production period of TSL would be advantageous for application and commercialization.…”

“…Other laccases from Cerrena sp. HYB07 (93.4 M) [30], T. trogii (69 M) [47], and Trametes sp. (50 M) [10] share close but higher K m values.…”

Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

“…The purification of laccase obtained in the present study is comparable with Chaurasia et al (2014) who reported purification (10.42-fold) with 12.57 % yield of laccase from Trametes hirsuta MTCC-1171 using DEAE cellulose column chromatography. However, Yan et al (2014) reported 1.37-and 4.07-fold purification with 5.78 and 11.64 % yield of laccase from T. trogii S0301 using anionic exchange chromatography followed by Sephadex G-75 chromatography, respectively. …”

Biodegradation and detoxification of textile dye Disperse Red 54 by Brevibacillus laterosporus and determination of its metabolic fate

“…Fungal species predominantly synthesize peroxidases during the degradation of dyes, that were induced in cells of Thanatephorus cucumeris and Phanerochaete sordida during biodegradation of anthraquinone, disperse, azo and phthalocyanine dyes (17,18). Laccase can nonspecifically oxidize various phenolic and non-phenolic compounds, using molecular oxygen as the final electron acceptor (22). Veratryl alcohol oxidase is known to carry out oxidative cleavage and desulphonation reactions (23,24).…”