Purification and Characterization Laccase from Trametes versicolor (L.) Lloyd in Submerged Fermentation

Yuliana, T.; Putri, Ratna E.; Hanidah, I.; Mardawati, E.; Tjaturina, H.

doi:10.3923/pjbs.2022.1077.1084

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…The laccase activity was spectrophotometrically determined as per the method of Yuliana et al [23]. A mixture of 60 µL of 0.5 M acetate buffer at pH 5, 20 µL of 1 mM 2.2 -azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and 50 µL of the crude enzyme was incubated in a microplate at 37 • C for 30 min, with absorbance measured at 420 nm.…”

Section: Enzyme Activity Testmentioning

confidence: 99%

Integrated Production of Xylitol, Ethanol, and Enzymes from Oil Palm Empty Fruit Bunch through Bioprocessing as an Application of the Biorefinery Concept

Mardawati,

Nawawi,

Caroline

et al. 2023

Fermentation

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Oil palm empty fruit bunch (OPEFB), an abundant source of lignocellulosic biomass waste, is rich in hemicellulose and is converted into xylose for xylitol production. The remaining cellulose-rich residue can be efficiently hydrolyzed into glucose, which serves as a substrate for bioethanol and enzymes. This process aligns with an integrated biorefinery model aimed at optimizing the utilization of OPEFB. This study optimizes a two-stage enzymatic hydrolysis fermentation for OPEFB conversion into value-added products. Using a 4% NaOH pretreatment, lignin was degraded while preserving hemicellulose and cellulose. This hydrolysis yielded 12.27 g/L of xylose and 36.86 g/L of glucose. Ethanol production, using varied fermentation media, achieved maximum concentrations of 0.043 g/L for xylitol and 21.35 g/L for ethanol, with substrate-to-product yields of 0.005 g/g and 0.374 g/g, respectively. Furthermore, enzyme production by Aspergillus niger was assessed on multiple parameters, recording a peak cellulase activity of 55.16 ± 20.24 U/mL and enzyme weight of 42.748 kDa. The OPEFB substrate yielded the highest protein content of 0.00942 ± 0.00010 mg/mL. These findings demonstrate the feasibility and efficiency of the two-stage enzymatic hydrolysis strategy in facilitating integrated biorefinery processes for efficient and sustainable OPEFB utilization.

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Section: Enzyme Activity Testmentioning

confidence: 99%

Integrated Production of Xylitol, Ethanol, and Enzymes from Oil Palm Empty Fruit Bunch through Bioprocessing as an Application of the Biorefinery Concept

Mardawati,

Nawawi,

Caroline

et al. 2023

Fermentation

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Advances in the Function and Properties of Laccase and Its Microbial Production

孙

2023

AMB

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Screening and Molecular Identification of Potential Lignolytic White Rot Fungi Isolated from Western Ghats

Suresh,

Ragunathan,

Johney

2023

Biosci., Biotech. Res. Asia

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The mounting concerns over the usage of enormous amount of wood and perilous chemicals in paper industry have led the researchers to develop ecofriendly green technologies for pulp and paper production. Lignocellulosic agro-waste can be an excellent alternative to the wood in papermaking. White rot fungi with ligninolytic properties can be effectively used for pretreatment of agro-wastes for pulp making. This study isolates and identifies previously unexplored strains of white rot fungi from Western Ghats of Kerala, India which can be used for developing biological systems for lignocellulosic treatment in pulp and paper production. Ten isolates were identified using molecular techniques and screened for their extracellular enzyme activities, including laccase, Manganese peroxidase and Lignin peroxidase. Among the 10 isolates, Trametes versicolor (Laccase activity 31.79 U/ml, Manganese peroxidase (MnP) 42.336 U/ml and Lignin peroxidase (LiP) - 50.65U/ml, Favolus teniculus (Laccase - 41.54 U/ml, MnP - 44.07 U/ml &LiP - 30.54), Coriolopsis byrsina (Laccase - 42.56 U/ml, MnP - 43.54 U/ml and LiP - 25.14 U/ml) Lenzitus betulina (Laccase - 37.15 U/ml, MnP - 38.97 U/ml and LiP - 30.43 U/ml) exhibited highly promising lignolytic enzymatic system. The study’s findings may provide a better eco-friendly substitute for conventional chemical treatments in various industrial applications.

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Purification and Characterization Laccase from Trametes versicolor (L.) Lloyd in Submerged Fermentation

Cited by 3 publications

References 20 publications

Integrated Production of Xylitol, Ethanol, and Enzymes from Oil Palm Empty Fruit Bunch through Bioprocessing as an Application of the Biorefinery Concept

Integrated Production of Xylitol, Ethanol, and Enzymes from Oil Palm Empty Fruit Bunch through Bioprocessing as an Application of the Biorefinery Concept

Advances in the Function and Properties of Laccase and Its Microbial Production

Screening and Molecular Identification of Potential Lignolytic White Rot Fungi Isolated from Western Ghats

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