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“…PPO activity was determined by measuring the rate of increase in absorbance at 420 nm, as described by Lima et al (2001) with modifications. The reaction mixture consisted of 2.9 mL of substrate solution (0.01 M catechol in 0.1 M phosphate buffer pH 7.0) and 0.1 mL of enzyme solution, at 30 °C.…”
Section: Enzyme Activity Determinationmentioning
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“…PPO activity was determined by measuring the rate of increase in absorbance at 420 nm, as described by Lima et al (2001) with modifications. The reaction mixture consisted of 2.9 mL of substrate solution (0.01 M catechol in 0.1 M phosphate buffer pH 7.0) and 0.1 mL of enzyme solution, at 30 °C.…”
Section: Enzyme Activity Determinationmentioning
“…The fruits showed low fat (0.38-0.58 g.100 g -1 ) and low protein (0.96-1.60 g.100 g -1 ) contents. Lima et al (2003) evaluated the physicochemical changes of soursop after harvest at physiological maturity and stored at ambient temperature for 6 days. After 6 days of storage it was obtained values of 0.88 g.100 g -1 for total acidity, 3.60 for pH and 14.40 °Brix for soluble solids.…”
Section: Sensory Evaluation Of Jamsmentioning
“…They are commonly rejected at market because of external injury or uneven shape and size. Therefore, incorporation of these tropical fruits pulps into nectars, jams and ice creams is a way to exploit their exotic flavors (LIMA et al, 2001;NEVES;YUHARA, 2003;UMME et al, 2001).…”
mentioning
“…As avaliações químicas realizadas foram: a) determinação dos teores de sólidos solúveis: em refratômetro digital, segundo Association of Official Analytical Chemistry (1992) e expressos os resultados em ºBrix; b) açúcares totais: pelo método do Instituto Adolfo Lutz (2008) e expressos os resultados em glicídios totais em glicose, por cento m/m; c) amido: pelo método do Instituto Adolfo Lutz (2008) e expressos os resultados em glicídios não redutores, em amido, por cento m/m e d) atividade enzimática da polifenoloxidase, método descrito por Lima, Pastore e Lima (2001), e da peroxidase, seguindo o método descrito por Clemente e Pastore (1998), e expressos os resultados em Absorbância/mL/min. Para determinar a atividade enzimática da polifenoloxidase (PPO) foi utilizado o método descrito por Lima, Pastore e Lima (2001). Após a mistura de 5.10 -4 L do extrato enzimático, com 8.10 -4 L de solução-tampão fosfato de sódio (1.10 -4 mol m -3 pH 6,0) e 5.10 -4 L de solução de catecol 1.10 -5 mol m -3 , a solução resultante foi incubada a 30 ºC por 1.800 s. Em seguida, acrescentou-se 8.10 -4 L de ácido perclórico 2.10 -3 mol m -3 .…”
Section: Methodsunclassified