PuraStat RADA16 Self-Assembling Peptide Reduces Postoperative Abdominal Adhesion Formation in a Rabbit Cecal Sidewall Injury Model

Gil, Eun Seok; Aleksi, Elton; Spirio, Lisa

doi:10.3389/fbioe.2021.782224

Cited by 8 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RADA16 functions as a topical hemostatic agent for surgery and prevents adhesion formation after surgery. [65] Previously the Hartgerink group studied diabetic wound healing in mice with SAPH composed of self-assembling domain without the biological mimics with significantly accelerated wound closure in their SAPH, K2, compared to a commercially available hydrogel, IntraSite, and buffer groups. [57] SAPHs, including RADA16 and K2, can be modified with various functional peptides to develop bioactive SAPHs imbued with the abilities of the appended mimetic sequence.…”

Section: Discussionmentioning

confidence: 99%

Angiogenic Hydrogels to Accelerate Early Wound Healing

Kim

Siddiqui

Acevedo‐Jake

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

Diabetes mellitus affects an increasing proportion of the population, and is projected to double by 2060. Comorbidities contribute to an interrupted healing process which is delayed, prolonged, and associated with increased susceptibility to infection and unresolved inflammation. This leads to chronic nonhealing wounds and potential amputation. Here, the use of a bioactive angiogenic peptide‐based hydrogel, SLan, is examined to improve early wound healing in diabetic rats, and its performance is compared to clinically utilized biosynthetic peptide‐based materials such as Puramatrix. Streptozotocin‐treated diabetic rats underwent 8 mm biopsy wounding in their dorsum. Wounds are treated with either Low (1 w%) SLan, High (4 w%) SLan, phosphate buffered saline (PBS), Puramatrix, or K2 (an unfunctionalized nonbioactive control sequentially similar to SLan), covered with Tegaderm, and monitored on for a month; animals are sacrificed for histomorphic analyses and immunostaining. Pharmacokinetic analysis showing no trafficking of peptides from the wound into the circulation. SLan groups show similar wound contraction as control groups (Puramatrix, PBS, and K2), however, showing marked improvement in healing in earlier time points, including increased deposition of new mature blood vessels. Altogether the results suggest this material can be used to “jumpstart” the diabetic wound healing process.

show abstract

Section: Discussionmentioning

confidence: 99%

Angiogenic Hydrogels to Accelerate Early Wound Healing

Kim

Siddiqui

Acevedo‐Jake

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Recently, Eun Seok Gil and co‐workers conducted one cecal sidewall abrasion surgery to evaluate post‐operative abdominal adhesion formation in rabbit animal model. [ 274 ] 2.5% RADA16 peptide formulation might achieve 79% higher mean mucosal coverage area in cecal sidewall wound sites in RADA16 peptide‐treated group than control ( p < 0.001). For the post‐operative management of peritoneal effusion in pelvic surgery, RADA16 peptide hydrogel exhibited effective hemostasis and safe tissue‐sealing quality superior to other topical hemostatic materials such as collagenous materials and fibrin glue.…”

Section: Clinical or Preclinical Use Of Dsap Biomaterials In Surgical...mentioning

confidence: 99%

Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

et al. 2023

View full text Add to dashboard Cite

Short designer self‐assembling peptide (dSAP) biomaterials are a new addition to the hemostat group. It may provide a diverse and robust toolbox for surgeons to integrate wound microenvironment with much safer and stronger hemostatic capacity than conventional materials and hemostatic agents. Especially in noncompressible torso hemorrhage (NCTH), diffuse mucosal surface bleeding, and internal medical bleeding (IMB), with respect to the optimal hemostatic formulation, dSAP biomaterials are the ingenious nanofiber alternatives to make bioactive neural scaffold, nasal packing, large mucosal surface coverage in gastrointestinal surgery (esophagus, gastric lesion, duodenum, and lower digestive tract), epicardiac cell‐delivery carrier, transparent matrix barrier, and so on. Herein, in multiple surgical specialties, dSAP‐biomaterial‐based nano‐hemostats achieve safe, effective, and immediate hemostasis, facile wound healing, and potentially reduce the risks in delayed bleeding, rebleeding, post‐operative bleeding, or related complications. The biosafety in vivo, bleeding indications, tissue‐sealing quality, surgical feasibility, and local usability are addressed comprehensively and sequentially and pursued to develop useful surgical techniques with better hemostatic performance. Here, the state of the art and all‐round advancements of nano‐hemostatic approaches in surgery are provided. Relevant critical insights will inspire exciting investigations on peptide nanotechnology, next‐generation biomaterials, and better promising prospects in clinics.

show abstract

“…We next assessed whether barrier material alone (e.g., hydrogel) is sufficient to prevent inflammatory/fibrotic processes during PA since SAP hydrogel (RADA16) has been shown to reduce PA to some extent. [10,12] KLD12 (n-KLDLKLDLKLDL-c) is an ionic-complementary SAP peptide containing altered positively charged lysines and negatively charged aspartic acids, and its net charge is zero. [25] To obtain a cationic SAP peptide, we added two arginines at the C-terminus of KLD12 (n-KLDLKLDLKLDLRR-c, KLD2R) as previously reported.…”

Section: Sap Hydrogel Alone As a Barrier Materials To Partially Atten...mentioning

confidence: 99%

“…In fact, a pilot study showed some positive effects of RADA16 hydrogel in preventing adhesion formation in a rabbit cecal sidewall injury model. [ 12 ] However, the beneficial roles of SAP hydrogel alone in preventing PA may be mainly due to its cross‐linked nanofiber structure that can cover injured sites, since its direct anti‐inflammatory or antifibrotic role is rarely reported. Thus, the biofunctions of SAP may be specifically improved by integration with bioactive components.…”

Section: Introductionmentioning

confidence: 99%

Injectable Multifunctional Composite Hydrogel as a Combination Therapy for Preventing Postsurgical Adhesion

Lv,

Lou,

Liu

et al. 2023

Small

View full text Add to dashboard Cite

Postsurgical adhesion (PA) is a common and serious postoperative complication that affects millions of patients worldwide. However, current commercial barrier materials are insufficient to inhibit diverse pathological factors during PA formation, and thus, highly bioactive materials are needed. Here, this work designs an injectable multifunctional composite hydrogel that can serve as a combination therapy for preventing PA. In brief, this work reveals that multiple pathological events, such as chronic inflammatory and fibrotic processes, contribute to adhesion formation in vivo, and such processes can not be attenuated by barrier material (e.g., hydrogel) alone treatments. To solve this limitation, this work designs a composite hydrogel made of the cationic self‐assembling peptide KLD2R and TGF‐β receptor inhibitor (TGF‐βRi)‐loaded mesenchymal stem cell‐derived nanovesicles (MSC‐NVs). The resulting composite hydrogel displays multiple functions, including physical separation of the injured tissue areas, antibacterial effects, and local delivery and sustained release of anti‐inflammatory MSC‐NVs and antifibrotic TGF‐βRi. As a result, this composite hydrogel effectively inhibited local inflammation, fibrosis and adhesion formation in vivo. Moreover, the hydrogel also exhibits good biocompatibility and biodegradability in vivo. Together, the results highlight that this “all‐in‐one” composite hydrogel strategy may provide insights into designing advanced therapies for many types of tissue injury.

show abstract

PuraStat RADA16 Self-Assembling Peptide Reduces Postoperative Abdominal Adhesion Formation in a Rabbit Cecal Sidewall Injury Model

Cited by 8 publications

References 34 publications

Angiogenic Hydrogels to Accelerate Early Wound Healing

Angiogenic Hydrogels to Accelerate Early Wound Healing

Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

Injectable Multifunctional Composite Hydrogel as a Combination Therapy for Preventing Postsurgical Adhesion

Contact Info

Product

Resources

About