PTX-loaded three-layer PLGA/CS/ALG nanoparticle based on layer-by-layer method for cancer therapy

Abstract

Poly (lactic-co-glycolic acid) (PLGA) nanoparticles are an ideal paclitaxel (PTX)-carrying system due to its biocompatibility and biodegradability. But it possessed disadvantage of drug burst release. In this research, a layer-by-layer deposition of chitosan (CS) and sodium alginate (ALG) was applied to modify the PLGA nanoparticles. The surface charges and morphology of the PLGA, PLGA/CS and PLGA/CS/ALG particles was measured by capillary electrophoresis and SEM and TEM, respectively. The drug encapsulation and loading efficiency were confirmed by ultraviolet spectrophotometer. The nanoparticles were stable and exhibited controlled drug release performance, with good cytotoxicity to human lung carcinoma cells (HepG 2). Cumulatively, our research suggests that this kind of three-layer nanoparticle with LbL-coated shield has great properties to act as a novel drug-loaded system.

Chitosan-Modified PLGA Nanoparticles for Control-Released Drug Delivery

“…However, one of the major drawbacks of PLGA NPs is that they cannot specifically interact with cells or proteins, which causes an inability to accumulate drugs in target tissues [4,5]. Another disadvantage of PLGA NPs is the presence of drug burst release, which could result in side effects [6,7].…”

Alginate Nanoformulation: Influence of Process and Selected Variables

“…The values of zeta potential of the above-mentioned nanoparticles were −2.72 ± 0.17 mV, +17.36 ± 0.84 mV and −10.62 ± 0.38 mV, respectively. The drug release study was carried out in PBS buffer (pH 7.4), and paclitaxel nanoparticles exhibited low initial burst release and prolonged release properties [ 44 ]. Khan et al, have investigated the influence of alginate/chitosan coat complex on the sustained release and bio-accessibility of resveratrol-zein nanoparticles [ 96 ].…”

“… Scanning electron microscopy images of alginate nanoparticles prepared by: ( a ) emulsification/internal gelation, 1% w / w alginate, CaCO 3 :alginate mass ratios 0.1:1, pH 6 medium chain triglyceride (MCT) oil [ 40 ]; ( b ) nanospray dryer, spray cap 7 µm, flow rate 7 mL/min, drying gas flow of 110 L/min with relative flow rate 100%, inlet drying gas temperature 120 °C and outlet temperature 35 °C [ 41 ]; ( c ) polyelectrolyte complexation, nisin-loaded nanoparticles alginate 250 mg/mL and chitosan 250 mg/mL [ 42 ]; ( d ) evaporation method, zein-to-propylene glycol, alginate mass ratio 20:1 [ 43 ]; ( e ) layer-by-layer, paclitaxel, poly (lactic-co-glycolic acid) (PLGA) 10 mg/mL, alginate 5 mg/mL and chitosan 5 mg/mL [ 44 ]; ( f ) emulsification/external gelation, alginate 0.03% w / v and CaCl 2 18 mM [ 45 ]; ( g ) electrospray DNA plasmid loaded alginate nanoparticles, flow rate 0.1 mL/h, voltage 12.5 kV, alginate 1% w / v , Tween 20 1% v / v , CaCl 2 1.5% w / v , collector distance 4 cm and nozzle size 30 G [ 46 ]; ( h ) nanofiber produced by electrospinning, alginate 1.74% w / w , voltage 12 kV, needle 27 G, flow rate 0.6 mL/h and distance 12 cm [ 47 ]. …”

Fabrication of Carboxylmethyl Chitosan Nanocarrier via Self-Assembly for Efficient Delivery of Phenylethyl Resorcinol in B16 Cells

“…Due to their biosafety and biodegradable character, self-assembled nanoparticles such as lipid, polylactic-co-glycolic acid (PLGA), polyamidoamine (PAMAM) dendrimers and polysaccharide based nanoparticles consisting of an internal hydrophobic core and an externally surrounded hydrophilic group, have been widely reported to increase the delivery efficiency and bioavailability of drugs [20][21][22][23]. Among these nanoparticles prepared from polymers, there has been rising interest in nanoscale self-aggregates of natural polysaccharides such as pullulan [24], curdlan [25], dextran [26], alginic acid [27], and chitosan [28]. Carboxymethyl chitosan (CMCS) is one of the water-soluble derivatives of chitosan, which has attracted the most attention and exploration in the field of biomedicine [29].…”