PtoMYB92 is a Transcriptional Activator of the Lignin Biosynthetic Pathway During Secondary Cell Wall Formation in<i>Populus tomentosa</i>

Li, Chaofeng; Wang, Xinqiang; Ran, Lingyu; Tian, Qiaoyan; Fan, Di; Luo, Keming

doi:10.1093/pcp/pcv157

Cited by 87 publications

(58 citation statements)

References 52 publications

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“…Two MYB transcription factors, AtMYB58 and AtMYB63, are well-known specific transcriptional activators of lignin biosynthesis during secondary cell wall formation in Arabidopsis [9,15,22]. Among several MYB transcription factors, PtrMYB152 and PtoMYB92 were reported to be involved in lignin biosynthesis in poplar [8,23,24]. The transcriptional levels of PtrMYB92 and PtoMYB152 were downregulated 43% and 53% compared to WT independently ( Figure 6A Figure 6B).…”

Section: Myb Transcription Factors and Lignin Biosynthesis Genes Are mentioning

confidence: 94%

Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

Jang

Lee

2020

Plants

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Lipid acyl hydrolase are a diverse group of enzymes that hydrolyze the ester or amide bonds of fatty acid in plant lipids. Patatin-related phospholipase AIIIs (pPLAIIIs) are one of major lipid acyl hydrolases that are less closely related to potato tuber patatins and are plant-specific. Recently, overexpression of ginseng-derived PgpPLAIIIβwas reported to be involved in the reduced level of lignin content in Arabidopsis and the mature xylem layer of poplar. The presence of lignin-polysaccharides renders cell walls recalcitrant for pulping and biofuel production. The tissue-specific regulation of lignin biosynthesis, without altering all xylem in plants, can be utilized usefully by keeping mechanical strength and resistance to various environmental stimuli. To identify another pPLAIII homolog from Arabidopsis, constitutively overexpressed AtpPLAIIIα was characterized for xylem lignification in two well-studied model plants, Arabidopsis and poplar. The characterization of gene function in annual and perennial plants with respect to lignin biosynthesis revealed the functional redundancy of less lignification via downregulation of lignin biosynthesis-related genes.

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Section: Myb Transcription Factors and Lignin Biosynthesis Genes Are mentioning

confidence: 94%

Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

Jang

Lee

2020

Plants

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“…Orthologs of MYB42 and MYB85 in poplar (Populus tomentosa) activate the lignin biosynthetic pathway during cell wall formation (Li et al, 2015a). Japanese cedar tree (Cryptomeria japonica) orthologs of Arabidopsis MYB20 and MYB43 are implicated in the regulation of secondary cell wall biosynthesis in the xylem (Mishima et al, 2014).…”

Section: Arabidopsis Myb99 Is One Of Six Myb Family Subclade Members mentioning

confidence: 99%

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

et al. 2019

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The pollen wall is a complex, durable structure essential for plant reproduction. A substantial portion of phenylpropanoids (e.g. flavonols) produced by pollen grain tapetal cells are deposited in the pollen wall. Transcriptional regulation of pollen wall formation has been studied extensively, and a specific regulatory mechanism for Arabidopsis (Arabidopsis thaliana) pollen flavonol biosynthesis has been postulated. Here, metabolome and transcriptome analyses of anthers from mutant and overexpression genotypes revealed that Arabidopsis MYB99, a putative ortholog of the petunia (Petunia hybrida) floral scent regulator ODORANT1 (ODO1), controls the exclusive production of tapetum diglycosylated flavonols and hydroxycinnamic acid amides. We discovered that MYB99 acts in a regulatory triad with MYB21 and MYB24, orthologs of emission of benzenoids I and II, which together with ODO1 coregulate petunia scent biosynthesis genes. Furthermore, promoter-activation assays showed that MYB99 directs precursor supply from the Calvin cycle and oxidative pentose-phosphate pathway in primary metabolism to phenylpropanoid biosynthesis by controlling TRANSKETOLASE2 expression. We provide a model depicting the relationship between the Arabidopsis MYB triad and structural genes from primary and phenylpropanoid metabolism and compare this mechanism with petunia scent control. The discovery of orthologous protein triads producing related secondary metabolites suggests that analogous regulatory modules exist in other plants and act to regulate various branches of the intricate phenylpropanoid pathway.

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“…Similarly, MsSCM1 falls into a clade of SCW regulators including AtMYB20/43/85, PbrMYB169, PtoMYB92, and PvMYB85 (Fig. 1; Zhong et al ., 2008; Li et al ., 2015; Rao et al ., 2018; Xue et al ., 2019).…”

Section: Resultsmentioning

confidence: 99%

Distinct and overlapping functions ofMiscanthus sinensisMYB transcription factors SCM1 and MYB103 in lignin biosynthesis

Golfier

Unda

Murphy

et al. 2019

Preprint

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38 Cell wall recalcitrance is a major constraint for the exploitation of lignocellulosic biomass as 39 renewable resource for energy and bio-based products. Transcriptional regulators of the lignin 40 biosynthetic pathway represent promising targets for tailoring lignin content and composition in 41 plant secondary cell walls. A wealth of research in model organisms has revealed that 42 transcriptional regulation of secondary cell wall formation is orchestrated by a hierarchical 43 transcription factor (TF) network with NAC TFs as master regulators and MYB factors in the lower 44 tier regulators. However, knowledge about the transcriptional regulation of lignin biosynthesis in 45 lignocellulosic feedstocks, such as Miscanthus, is limited. Here, we characterized two Miscanthus 46 MYB TFs, MsSCM1 and MsMYB103, and compared their transcriptional impact with that of the 47 master regulator MsSND1. In Miscanthus leaves MsSCM1 and MsMYB103 are expressed at 48 growth stages associated with lignification. Ectopic expression of MsSCM1 and MsMYB103 in 49 tobacco leaves was sufficient to trigger secondary cell wall deposition with distinct sugar and lignin 50 composition. Moreover, RNA-seq analysis revealed that the transcriptional responses to MsSCM1 51 and MsMYB103 overexpression showed extensive overlap with the response to MsSND1, but 52 were distinct from each other, underscoring the inherent complexity of secondary cell wall 53 formation. Together, MsSCM1 and MsMYB103 represent interesting targets for manipulations of 54 lignin content and composition in Miscanthus towards tailored biomass. 55 56 157 158 Tissue Staining and Microscopy 159

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PtoMYB92 is a Transcriptional Activator of the Lignin Biosynthetic Pathway During Secondary Cell Wall Formation inPopulus tomentosa

Cited by 87 publications

References 52 publications

Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

Distinct and overlapping functions ofMiscanthus sinensisMYB transcription factors SCM1 and MYB103 in lignin biosynthesis

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