Pseudomonas burn wound sepsis. II

Teplitz, Carl; Davis, Donald W.; Walker, Harrel L.; Raulston, Gilbert L.; Mason, Arthur D.; Moncrief, John A.

doi:10.1016/s0022-4804(64)80027-5

Cited by 50 publications

(11 citation statements)

References 2 publications

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“…The surge of the systemic inflammatory response to burn has been linked to circulating damage-associated molecular patterns (DAMPs) following thermal injury (37). Besides this, P. aeruginosa burn wound infection in rodent models has long been recognized to progress from wound colonization to hematogenous dissemination (38,39). Consistent with these data, in our study, superimposition of PA14 infection on thermal injury led to a significant rise of the systemic inflammatory response, as well as P. aeruginosa systemic dissemination.…”

Section: Resultsmentioning

confidence: 99%

Targeting bacterial quorum sensing shows promise in improving intestinal barrier function following burn‑site infection

et al. 2019

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Burn-site infections, commonly due to Pseudomonas aeruginosa , have been associated with deranged intestinal integrity, allowing bacteria and their products to translocate from the gut to the circulatory system. The P. aeruginosa quorum sensing (QS) transcription factor MvfR (PqsR) controls the expression of numerous virulence factors, and the synthesis of several toxic products. However, the role of QS in intestinal integrity alterations, to the best of our knowledge, has not been previously investigated. Using a proven anti-MvfR, anti-virulence agent, the in vivo results of the present study revealed that inhibition of MvfR function significantly decreased Fluorescein Isothiocyanate-Dextran (FITC-Dextran) flow from the intestine to the systemic circulation, diminished bacterial translocation from the intestine to mesenteric lymph nodes (MLNs), and improved tight junction integrity in thermally injured and infected mice. In addition, the MvfR antagonist administration alleviates the intestinal inflammation, as demonstrated by reduced ileal TNF-α and fecal lipocalin-2 concentrations. In addition, it is associated with lower levels of circulating endotoxin and decreased P. aeruginosa dissemination from the burn wound to the ileum. Collectively, these results hold great promise that the inhibition of this QS system mitigates gut hyperpermeability by attenuating the derangement of morphological and immune aspects of the intestinal barrier, suggesting that MvfR function is crucial in the deterioration of intestinal integrity following P. aeruginosa burn-site infection. Therefore, an anti-virulence approach targeting MvfR, could potentially offer a novel therapeutic approach against multi-drug resistant P. aeruginosa infections following thermal injuries. Since this approach is targeting virulence pathways that are non-essential for growth or viability, our strategy is hypothesized to minimize the development of bacterial resistance, and preserve the beneficial enteric microbes, while improving intestinal integrity that is deranged as a result of burn and infection.

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Section: Resultsmentioning

confidence: 99%

Targeting bacterial quorum sensing shows promise in improving intestinal barrier function following burn‑site infection

et al. 2019

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“…Furthermore, the risk of serious infections, particularly septicemias, increases with extended hospitalization (23). Because of its antibiotic resistance and ubiquitous distri-bution in the hospital environment, P. aeruginosa is one of the most dangerous opportunistic pathogens (3,23,26,31,32). The need for a simple, reliable technique for comparing all "in-house" isolates of P. aeruginosa is evident.…”

Section: Resultsmentioning

confidence: 99%

Pyocin Typing of Pseudomonas aeruginosa: a Simplified Method

Jones¹,

Zakanycz²,

Thomas³

et al. 1974

Applied Microbiology

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A simplified method has been devised for typing Pseudomonas aeruginosa by pyocin production. Pyocins are produced as strains grow overnight in Trypticase soy broth (without glucose) plus 1% potassium nitrate. Because P. aeruginosa can use nitrate instead of oxygen as a terminal electron acceptor, mechanical shaking is not necessary, nor is induction by mitomycin C. Pyocins can now be produced in screw-cap tubes in a water bath or incubator. A total of 250 strains were tested as possible pyocin indicators, which included 60 strains already used in pyocin-typing systems. The final set contained 18 indicators which were chosen because (i) they had clear positive or clear negative reactions, thus eliminating reactions difficult to read, (ii) they had few zones due to bacteriophage lysis, and (iii) they were most sensitive in differentiating clinical isolates of P. aeruginosa. The final typing method was tested in several studies and the results were clear; thus definitive epidemiological conclusions could be made. Because it is simple to perform and easily automated, the new method should have application in many hospitals; however, it should be used only in carefully planned epidemiological studies. The method and its application are described in detail, and some pitfalls are discussed. Nosocomial infections caused by Pseudomonas aeruginosa continue to be a problem in many hospitals (3, 5, 8, 12, 16, 23). Within any institution there can be hundreds of different strains because it is often found in the human gut, and because it can survive and multiply in moist environments of the hospital. In any epidemiological study, a sensitive technique is required to compare these numerous strains. A number of methods have been used to differentiate P. aeruginosa in epidemiological studies. These epidemiological markers include

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“…Damage to this barrier following a burn disrupts the innate immune system and increases susceptibility to bacterial infection [61]. Burn wound infection was defined in a rat model with Pseudomonas aeruginosa [62, 63], in which the following progression was observed: burn wound colonization; invasion into subjacent tissue within 5 days; destruction of granulation tissue; visceral hematogenous lesions; and leukopenia, hypothermia, and death. Burn patients are at high risk for infection [64], especially drug-resistant infection [65], which often results in significantly longer hospital stays, delayed wound healing, higher costs, and higher mortality [66].…”

Section: Optimization Of Burn Wound Healingmentioning

confidence: 99%

Burn wound healing and treatment: review and advancements

et al. 2015

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Burns are a prevalent and burdensome critical care problem. The priorities of specialized facilities focus on stabilizing the patient, preventing infection, and optimizing functional recovery. Research on burns has generated sustained interest over the past few decades, and several important advancements have resulted in more effective patient stabilization and decreased mortality, especially among young patients and those with burns of intermediate extent. However, for the intensivist, challenges often exist that complicate patient support and stabilization. Furthermore, burn wounds are complex and can present unique difficulties that require late intervention or life-long rehabilitation. In addition to improvements in patient stabilization and care, research in burn wound care has yielded advancements that will continue to improve functional recovery. This article reviews recent advancements in the care of burn patients with a focus on the pathophysiology and treatment of burn wounds.

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Pseudomonas burn wound sepsis. II

Cited by 50 publications

References 2 publications

Targeting bacterial quorum sensing shows promise in improving intestinal barrier function following burn‑site infection

Targeting bacterial quorum sensing shows promise in improving intestinal barrier function following burn‑site infection

Pyocin Typing of Pseudomonas aeruginosa: a Simplified Method

Burn wound healing and treatment: review and advancements

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