Pseudomonas Attachment to New Hydrogel Contact Lenses

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

“…In the current study, viable bacterial numbers on the silicone CLs decreased within the first few hours, an observation that contrasts with some previous studies [19,25,26,33,56], which have generally reported a continuous increase of initial bacterial adherence. One possible explanation for this is that, in contrast to many previous studies, the artificial tear fluid used here contained antimicrobial components such as lysozyme, which may lead to an initial viable cell reduction before physiological adaptation of the bacteria could occur.…”

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

“…In the current study, viable bacterial numbers on the silicone CLs decreased within the first few hours, an observation that contrasts with some previous studies [19,25,26,33,56], which have generally reported a continuous increase of initial bacterial adherence. One possible explanation for this is that, in contrast to many previous studies, the artificial tear fluid used here contained antimicrobial components such as lysozyme, which may lead to an initial viable cell reduction before physiological adaptation of the bacteria could occur.…”

A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses

“…Hence, in the ocular system, introduction of an environmental strain can potentially result in lens colonization and subsequent infection. Differences in adhesion levels between Pseudomonas strains were similar to those reported in other studies (Duran et al 1987). These strains, however, always adhered in higher numbers than the other ocular strains, including the Ser.…”

“…As the contact lens may not be fully coated until several hours after insertion it is important to consider the material of the contact lens in adhesion, not only the biofilm that will ultimately form on it. Pseudomonas aeruginosa has been shown to attach to new, unworn hydrogel lenses in addition to worn contact lenses (Duran et al 1987). In this study it was demonstrated that bacterial adhesion to worn lenses also increased with increasing NaCl concentration as observed for clean lenses.…”

Surface AFM microscopy of unworn and worn samples of silicone hydrogel contact lenses

“…These results agree with the findings of Boles et al who concluded that worn disposable CLs restricted the attachment of Pseudomonas aeruginosa compared to new lenses 24. Early studies from Duran et al also support the affinity of new CL materials for bacterial adhesion 25. Regarding the lack of significance of increase in surface roughness found by Vermelfoort et al23 in their study, this seems not to be supported by previous research that demonstrated a significant increase in roughness 10, 26.…”

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

“…In the current study, viable bacterial numbers on the silicone CLs decreased within the first few hours, an observation that contrasts with some previous studies [19,25,26,33,56], which have generally reported a continuous increase of initial bacterial adherence. One possible explanation for this is that, in contrast to many previous studies, the artificial tear fluid used here contained antimicrobial components such as lysozyme, which may lead to an initial viable cell reduction before physiological adaptation of the bacteria could occur.…”

A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses

“…Hence, in the ocular system, introduction of an environmental strain can potentially result in lens colonization and subsequent infection. Differences in adhesion levels between Pseudomonas strains were similar to those reported in other studies (Duran et al 1987). These strains, however, always adhered in higher numbers than the other ocular strains, including the Ser.…”

“…As the contact lens may not be fully coated until several hours after insertion it is important to consider the material of the contact lens in adhesion, not only the biofilm that will ultimately form on it. Pseudomonas aeruginosa has been shown to attach to new, unworn hydrogel lenses in addition to worn contact lenses (Duran et al 1987). In this study it was demonstrated that bacterial adhesion to worn lenses also increased with increasing NaCl concentration as observed for clean lenses.…”

Surface AFM microscopy of unworn and worn samples of silicone hydrogel contact lenses

“…These results agree with the findings of Boles et al who concluded that worn disposable CLs restricted the attachment of Pseudomonas aeruginosa compared to new lenses 24. Early studies from Duran et al also support the affinity of new CL materials for bacterial adhesion 25. Regarding the lack of significance of increase in surface roughness found by Vermelfoort et al23 in their study, this seems not to be supported by previous research that demonstrated a significant increase in roughness 10, 26.…”

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

“…In the current study, viable bacterial numbers on the silicone CLs decreased within the first few hours, an observation that contrasts with some previous studies [19,25,26,33,56], which have generally reported a continuous increase of initial bacterial adherence. One possible explanation for this is that, in contrast to many previous studies, the artificial tear fluid used here contained antimicrobial components such as lysozyme, which may lead to an initial viable cell reduction before physiological adaptation of the bacteria could occur.…”

A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses

“…Hence, in the ocular system, introduction of an environmental strain can potentially result in lens colonization and subsequent infection. Differences in adhesion levels between Pseudomonas strains were similar to those reported in other studies (Duran et al 1987). These strains, however, always adhered in higher numbers than the other ocular strains, including the Ser.…”

“…As the contact lens may not be fully coated until several hours after insertion it is important to consider the material of the contact lens in adhesion, not only the biofilm that will ultimately form on it. Pseudomonas aeruginosa has been shown to attach to new, unworn hydrogel lenses in addition to worn contact lenses (Duran et al 1987). In this study it was demonstrated that bacterial adhesion to worn lenses also increased with increasing NaCl concentration as observed for clean lenses.…”

Surface AFM microscopy of unworn and worn samples of silicone hydrogel contact lenses

“…These results agree with the findings of Boles et al who concluded that worn disposable CLs restricted the attachment of Pseudomonas aeruginosa compared to new lenses 24. Early studies from Duran et al also support the affinity of new CL materials for bacterial adhesion 25. Regarding the lack of significance of increase in surface roughness found by Vermelfoort et al23 in their study, this seems not to be supported by previous research that demonstrated a significant increase in roughness 10, 26.…”